国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (5): 539-545.doi: 10.7518/gjkq.2018.05.008

• 论著 • 上一篇    下一篇

钛合金表面氧化石墨烯涂层的制备

邓雪阳,潘兰兰,胡婷,李文华,向学熔()   

  1. 重庆医科大学附属口腔医院牙周科 口腔疾病与生物医学重庆市重点实验室 重庆市高校市级口腔生物医学工程重点实验室 重庆 401147
  • 收稿日期:2017-10-19 修回日期:2018-04-10 出版日期:2018-09-01 发布日期:2018-09-20
  • 通讯作者: 向学熔 E-mail:cqzxfxp@sina.com
  • 作者简介:邓雪阳,硕士,Email:gushuzi188@sina.com
  • 基金资助:
    重庆医科大学国家自然科学基金预研项目(NSFYY20151- 6);重庆市渝北区科技计划项目(2015 Social 10);重庆市高校创新团队建设计划资助项目(CXTDG201602006)

Preparation of graphene oxide coatings on titanium alloy surface

Xueyang Deng,Lanlan Pan,Ting Hu,Wenhua Li,Xuerong. Xiang()   

  1. Dept. of Periodontology, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2017-10-19 Revised:2018-04-10 Online:2018-09-01 Published:2018-09-20
  • Contact: Xuerong. Xiang E-mail:cqzxfxp@sina.com
  • Supported by:
    This study was supported by Pre-Research Project of National Natural Science Foundation of China in Chongqing Medical University(NSFYY20151- 6);Science and Technology Planning Project in Yubei District of Chongqing(2015 Social 10);Project Supported by Program for Innovation Team Building at Institutions of Higher Education in Chongqing(CXTDG201602006)

摘要:

目的 利用氧化石墨烯(GO)制备钛合金表面涂层,初步评价其生物相容性。方法 采用层层自组装的方法,在碱热处理后的钛合金表面形成由GO阳离子(GO-NH3 +)、GO阴离子(GO-COO -)溶液组成的氧化石墨烯涂层。使用接触角测量仪、扫描电子显微镜、原子力显微镜、万能材料试验机初步分析其亲水性、表面形貌、涂层厚度、涂层粘接强度等特征,扫描电子显微镜、荧光显微镜观察骨髓间充质干细胞在其表面的生长情况,贴膜实验观察其抑菌性能。 结果 通过层层自组装方法在碱热处理后的钛合金表面制备出具有较好亲水性和粘接强度的、纳米级形貌的GO涂层。骨髓间充质干细胞在具有GO涂层的钛片的表面易于黏附,而且骨髓间充质干细胞活性保持良好。GO涂层对牙龈卟啉单胞菌的杀菌率为86.34%。结论 GO涂层的钛合金有良好的亲水性、生物相容性及抗菌性。

关键词: 氧化石墨烯, 表面涂层, 生物相容性, 抑菌

Abstract:

Objective To prepare a kind of coating on the titanium alloy surface with graphene oxide (GO) and to evaluate its biocompatibility preliminarily. Methods Ti substrates prepared by the alkali-hydrothermal reaction were coated with GO (Ti/GO) through layer-by-layer assembly of positively (GO-NH3 +) and negatively (GO-COO -) charged GO sheet with fifteen bilayers. The hydrophobic nature of GO coating on Ti substrates was analyzed by contact angle meter, and the microstructure of GO coating on Ti substrates were characterized by scanning electron microscope (SEM) and atomic force microscope (AFM). The in vitro cellular response was observed by the biological behaviors of bone marrow mesenchymal stem cells (BMSCs) which were inoculated on Ti substrates by SEM and fluorescence microscopy. Porphyromonas gingivalis was utilized to test the antibacterial effect of the Ti/GO surface. Results The GO coating with nano-scale surface topography on the aikali-hydrothermal reacted Ti alloy discs which was hydrophilic was prepared. The GO coating improved the adhension of BMSCs and kept cells live. 86.34% of bacteria had been killed on the Ti/GO surface. Conclusion The GO-coated Ti discs had good biocompatibility and antibacterial property.

Key words: graphene oxide, surface coating, biocom-patibility, antibacterial property

中图分类号: 

  • R783.1

图 1

钛片肉眼观察情况A:“镜面样”钛片;B:Ti组钛片;C:Ti/GO组钛片。"

图 2

钛片的SEM照片A、B:Ti组钛片;C、D:Ti/GO组钛片。放大倍数:A、C × 2 000,B、D × 20 000。"

图 3

Ti组、Ti/GO组钛片的AFM观察A、B:Ti组、Ti/GO组AFM照片,扫描区域为1.0 μm ×1.0 μm;C:Ti /GO组表面的三维形貌。"

图 4

接种于Ti组、Ti/GO组钛片表面BMSC的电镜图片A、B:Ti组钛片;C、D:Ti/GO组钛片。放大倍数A、C × 600;B、D × 1 200。"

图 5

材料表面BMSC的DAPI染色A:Ti组钛片;B:Ti/GO组钛片。箭头所示为钛片边缘。"

图 6

材料表面培养24 h后BMSC的双重染色 A:Ti组钛片表面的活细胞;B:Ti组钛片表面的死细胞;C:Ti/GO组钛片表面的活细胞;D:Ti/GO组钛片表面的死细胞。活细胞呈绿色,死细胞则染成红色。"

[1] Brånemark PI, Hansson BO, Adell R , et al. Os-seointegrated implants in the treatment of the eden-tulous jaw. Experience from a 10-year period[J]. Scand J Plast Reconstr Surg Suppl, 1977,16:1-132.
[2] Wang G, Moya S, Lu Z , et al. Enhancing orthopedic implant bioactivity: refining the nanotopography[J]. Nanomedicine (Lond), 2015,10(8):1327-1341.
doi: 10.2217/nnm.14.216 pmid: 25955126
[3] Wu SY, An SS, Hulme J , Current applications of graphene oxide in nanomedicine[J]. Int J Nanome-dicine, 2015,10(Spec Iss):9-24.
doi: 10.2147/IJN.S88285 pmid: 4554423
[4] Dubey N, Bentini R, Islam I , et al. Graphene: a ve-rsatile carbon-based material for bone tissue en-gineering[J]. Stem Cells Int, 2015,2015:804213.
[5] Sahni D, Jea A, Mata JA , et al. Biocompatibility of pristine graphene for neuronal interface[J]. J Neuro-surg Pediatr, 2013,11(5):575-583.
doi: 10.3171/2013.1.PEDS12374 pmid: 23473006
[6] Shen H, Zhang L, Liu M , et al. Biomedical applica-tions of graphene[J]. Theranostics, 2012,2(3):283-294.
doi: 10.7150/thno.3642
[7] Akhavan O, Ghaderi E , Toxicity of graphene and graphene oxide nanowalls against bacteria[J]. ACS Nano, 2010,4(10):5731-5736.
doi: 10.1021/nn101390x pmid: 20925398
[8] La WG, Jin M, Park S , et al. Delivery of bone mor-phogenetic protein-2 and substance P using graphene oxide for bone regeneration[J]. Int J Nanomedicine, 2014,9(Suppl 1):107-116.
[9] Grieznis L, Apse P, Blumfelds L , Passive tactile sensibility of teeth and osseointegrated dental im-plants in the maxilla[J]. Stomatologija, 2010,12(3):80-86.
pmid: 21063137
[10] Jemat A, Ghazali MJ, Razali M , et al. Surface modi-fications and their effects on titanium dental implants[J]. Biomed Res Int, 2015,2015:791725.
[11] Smeets R, Stadlinger B, Schwarz F , et al. Impact of dental implant surface modifications on osseointe-gration[J]. Biomed Res Int, 2016,2016:6285620.
[12] Gentile P, Carmagnola I, Nardo T , et al. Layer-by-layer assembly for biomedical applications in the last decade[J]. Nanotechnology, 2015,26(42):422001.
doi: 10.1088/0957-4484/26/42/422001 pmid: 26421916
[13] Park JS, Cho SM, Kim WJ , et al. Fabrication of gra-phene thin films based on layer-by-layer self-assembly of functionalized graphene nanosheets[J]. ACS Appl Mater Interfaces, 2011,3(2):360-368.
doi: 10.1021/am100977p
[14] La WG, Park S, Yoon HH , et al. Delivery of a the-rapeutic protein for bone regeneration from a sub-strate coated with graphene oxide[J]. Small, 2013,9(23):4051-4060.
doi: 10.1002/smll.201300571
[15] 吴昊, 刘洪臣 . 种植体周围炎的易感因素研究现状[J]. 口腔颌面修复学杂志, 2012,13(3):184-187.
Wu H, Liu HC . Research status of possible risk fac-tors of peri-implantitis: a review[J]. Chin J Prostho-dont, 2012,13(3):184-187.
[16] Persson GR, Renvert S , Cluster of bacteria asso-ciated with peri-implantitis[J]. Clin Implant Dent Relat Res, 2014,16(6):783-793.
doi: 10.1111/cid.12052 pmid: 23527870
[17] Liu S, Zeng TH, Hofmann M , et al. Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxida-tive stress[J]. ACS Nano, 2011,5(9):6971-6980.
doi: 10.1021/nn202451x
[18] Tu Y, Lv M, Xiu P , et al. Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets[J]. Nat Nanotechnol, 2013,8(8):594-601.
doi: 10.1038/nnano.2013.125 pmid: 23832191
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