Int J Stomatol

Inter J Stomatol ›› 2016, Vol. 43 ›› Issue (5): 578-583.doi: 10.7518/gjkq.2016.05.019

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Research progress on the knockout techniques of prokaryotic microorganisms

Yang Yan1, Yang Yingming1,2, Hu Tao1,2   

  1. 1. State Key Laboratory of Oral Diseases, Dept. of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Chengdu 610041, China; 2. State Key Laboratory of Oral Diseases, Dept. of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2015-09-19 Revised:2016-03-26 Online:2016-09-01 Published:2016-09-01

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Abstract: Gene knockout is a new molecular technique that developed in 1980s which has plays crucial roles in the microbiology functional and basic research. Through the development of several decades, gene knockout technique has been evolved from traditional homogenous recombination to λRed recombination and Cre-loxP system. Recently, the clustered regulatory interspaced short palindromic repeat/Cas9 system has been thought to be a very promising methods for microbiology manipulation for its’ convenience and effectiveness based on the activity of endonuclease. This review will focus on the gene deletion technique that has been applied in the microbiology research from the mechanism, application and prospect.

Key words: gene knockout, polymerase chain reaction, ligation mutagenesis, λRed recombination, Cre-loxP recombination, clustered regularly interspaced short palindromic repeat/Cas9

CLC Number: 

  • Q 756

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