Int J Stomatol

Inter J Stomatol ›› 2015, Vol. 42 ›› Issue (3): 290-293.doi: 10.7518/gjkq.2015.03.011

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The spatial-temporal changes in expression of thyroid transcription factor-2 during development of C57BL/6J mouse palate

Huang Lei1, Ni Xuying1, Shi Bing2, Zheng Qian2, Meng Tian2, Wang Yan2   

  1. 1. Dept. of Stomatology, Guangzhou First People’s Hospital, Guangzhou 510016, China; 2. State Key Laboratory of Oral Diseases, Dept. of Cleft Lip and Palate Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2014-12-12 Revised:2015-02-04 Online:2015-05-01 Published:2015-05-01

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Abstract:

Objective To explore the spatial-temporal regularity in expression of thyroid transcription factor-2(TTF-2) and to understand its role during development of C57BL/6J mouse palate. Methods The palatal shelves from the C57BL/6J mouse embryos were microdissected in aseptic condition on days 12, 13, and 14 of gestation. Each day, six pregnant mice were sacrificed. The reverse transcription-polymerase chain reaction(RT-PCR), Western blot and immunohistochemistry methods were used to detect semi-quantitative and qualitative expression of TTF-2 in palatal shelves. Results In the process of the C57BL/6J mouse palatal shelves growth(embryonic 12 day, E12), elevation(E13) and touch(E14), TTF-2 was expressed in palatal medial edge epithelium(MEE), but no TTF-2 was found in palatal mesenchymal cells by immunohistochemistry location. TTF-2 was not expressed in the MEE of shelf fusing(E15). A significant difference was found, and the highest expression was obtained at E13(P<0.05). This finding was consistent with the RT-PCR and Western blot results on the expression of TTF-2 in the palatal shelves of E12, E13, and E14. Conclusion The expression of TTF-2 during development of C57BL/6J mouse palatal shelves showed spatial-temporal changes correlating with cell differentiation and transformation.

Key words: thyroid transcription factor-2, palatal medial edge epithelium, reverse transcription-polymerase chain reaction, immunohistochemistry

CLC Number: 

  • R 782.2

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