Int J Stomatol

Int J Stomatol ›› 2025, Vol. 52 ›› Issue (6): 816-822.doi: 10.7518/gjkq.2025064

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Research progress on cold atmosphere plasma in oral tissue regeneration

Lin Yao1(),Ling Zou1,Xian Liu2()   

  1. 1.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Dept. of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Dept. of Emergency Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2024-06-30 Revised:2025-02-21 Online:2025-11-01 Published:2025-10-23
  • Contact: Xian Liu E-mail:yaolincc@163.com;Jssyliuxian@163.com
  • Supported by:
    the Natural Science Foundation of Sichuan Province(2024NSFSC0536)

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Abstract:

Cold atmospheric plasma (CAP) is an ionized gas that works under low temperatures and atmospheric pressure. It contains highly active reactive oxygen species and reactive nitrogen species, which play important roles in cell signaling, immune response, and other physiological processes. The application of CAP in stomatology has received increa-sing attention due to its excellent antibacterial, anti-inflammatory, and tissue-repairing abilities, which contribute to wound healing in the oral cavity. Furthermore, its surface modification ability can enhance the compatibility between dental tissues or implants and surrounding tissues. Therefore, CAP displays broad application prospects in the regeneration of oral soft and hard tissues, alleviation of oral disease symptoms, shortening of treatment course, and improvement of therapeutic effect. This article reviews the research progress on the utilization of CAP in oral tissue regeneration to provide a refe-rence for its clinical application.

Key words: cold atmospheric plasma, mucosal tissue regeneration, bone tissue regeneration, material surface modification

CLC Number: 

  • R78

TrendMD: 
[1] Sakudo A, Yagyu Y, Onodera T. Disinfection and sterilization using plasma technology: fundamentals and future perspectives for biological applications[J]. Int J Mol Sci, 2019, 20(20): 5216.
[2] Bernhardt T, Semmler ML, Schäfer M, et al. Plasma medicine: applications of cold atmospheric pressure plasma in dermatology[J]. Oxid Med Cell Longev, 2019, 2019: 3873928.
[3] Zarkovic N. Roles and functions of ROS and RNS in cellular physiology and pathology[J]. Cells, 2020, 9(3): 767.
[4] Laroussi M. Cold plasma in medicine and healthcare: the new frontier in low temperature plasma applications[J]. Front Phys, 2020, 8: 74.
[5] Gaharwar AK, Singh I, Khademhosseini A. Engineered biomaterials for in situ tissue regeneration[J]. Nat Rev Mater, 2020, 5(9): 686-705.
[6] Matzkeit N, Schulz L, Schleusser S, et al. Cold atmospheric plasma improves cutaneous microcirculation in standardized acute wounds: results of a controlled, prospective cohort study[J]. Microvasc Res, 2021, 138: 104211.
[7] Moelleken M, Jockenhöfer F, Wiegand C, et al. Pilotstudie zum einfluss von kaltem atmosphärischem plasma auf bakterielle kontamination und heilungstendenz chronischer wunden[J]. J Dtsch Dermatol Ges, 2020, 18(10): 1094-1102.
[8] Zhang JP, Guo L, Chen QL, et al. Effects and me-chanisms of cold atmospheric plasma on skin wound healing of rats[J]. Contrib Plasma Phys, 2019, 59(1): 92-101.
[9] Park J, Lee H, Lee HJ, et al. Non-thermal atmospheric pressure plasma efficiently promotes the proliferation of adipose tissue-derived stem cells by activating NO-response pathways[J]. Sci Rep, 2016, 6: 39298.
[10] Kokai LE, Marra K, Rubin JP. Adipose stem cells: biology and clinical applications for tissue repair and regeneration[J]. Transl Res, 2014, 163(4): 399-408.
[11] Park J, Lee H, Lee HJ, et al. Non-thermal atmospheric pressure plasma is an excellent tool to activate proliferation in various mesoderm-derived human adult stem cells[J]. Free Radic Biol Med, 2019, 134: 374-384.
[12] Kobayashi M, Tomoda K, Morihara H, et al. Non-thermal atmospheric-pressure plasma potentiates mesodermal differentiation of human induced pluripotent stem cells[J]. Heliyon, 2022, 8(12): e12009.
[13] Bhattacharjee B, Bezbaruah R, Rynjah D, et al. Cold atmospheric plasma: a noteworthy approach in medical science[J]. Sciphar, 2023, 2(2): 46-76.
[14] Przekora A, Audemar M, Pawlat J, et al. Positive effect of cold atmospheric nitrogen plasma on the behavior of mesenchymal stem cells cultured on a bone scaffold containing iron oxide-loaded silica nanoparticles catalyst[J]. Int J Mol Sci, 2020, 21(13): 4738.
[15] Wang M, Cheng XQ, Zhu W, et al. Design of biomimetic and bioactive cold plasma-modified nanostructured scaffolds for enhanced osteogenic diffe-rentiation of bone marrow-derived mesenchymal stem cells[J]. Tissue Eng Part A, 2014, 20(5/6): 1060-1071.
[16] Alemi PS, Atyabi SA, Sharifi F, et al. Synergistic effect of pressure cold atmospheric plasma and carboxymethyl chitosan to mesenchymal stem cell differentiation on PCL/CMC nanofibers for cartilage tissue engineering[J]. Polym Adv Technol, 2019, 30(6): 1356-1364.
[17] Preissner S, Poehlmann AC, Schubert A, et al. Ex vivo study comparing three cold atmospheric plasma (CAP) sources for biofilm removal on microstructured titanium[J]. Plasma Med, 2019, 9(1): 1-13.
[18] de Morais Gouvêa Lima G, Carta CFL, Borges AC, et al. Cold atmospheric pressure plasma is effective against P. gingivalis (HW24D-1) mature biofilms and non-genotoxic to oral cells[J]. Appl Sci, 2022, 12(14): 7247.
[19] Lima GMG, Borges AC, Nishime TMC, et al. Cold atmospheric plasma jet as a possible adjuvant therapy for periodontal disease[J]. Molecules, 2021, 26(18): 5590.
[20] Kleineidam B, Nokhbehsaim M, Deschner J, et al. Effect of cold plasma on periodontal wound healing-an in vitro study[J]. Clin Oral Investig, 2019, 23(4): 1941-1950.
[21] Eggers B, Stope MB, Marciniak J, et al. Modulation of inflammatory responses by a non-invasive physical plasma jet during gingival wound healing[J]. Cells, 2022, 11(17): 2740.
[22] Kusakcı-Seker B, Ozdemir H, Karadeniz-Saygili S. Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis[J]. Clin Oral Investig, 2021, 25(12): 6949-6959.
[23] Choi BB, Choi JH, Kang TH, et al. Enhancement of osteoblast differentiation using No-ozone cold plasma on human periodontal ligament cells[J]. Biome-dicines, 2021, 9(11): 1542.
[24] Eggers B, Marciniak J, Deschner J, et al. Cold atmospheric plasma promotes regeneration-associated cell functions of murine cementoblasts in vitro [J]. Int J Mol Sci, 2021, 22(10): 5280.
[25] Li Y, Liu YJ, Wang SB, et al. Non-thermal bio-compatible plasma induces osteogenic differentiation of human mesenchymal stem/stromal cells with ROS-induced activation of MAPK[J]. IEEE Access, 2020, 8: 36652-36663.
[26] Özdemir H, Seker B, Saygili S. Preventive effect of atmospheric cold plasma on alveolar bone loss in experimental periodontitis in rats[J]. Clin Oral Implants Res, 2019, 30(S19): 212.
[27] Liu ZX, Du XJ, Xu LY, et al. The therapeutic perspective of cold atmospheric plasma in periodontal disease[J]. Oral Dis, 2024, 30(3): 938-948.
[28] Zhang YX, Xiong Y, Xie P, et al. Non-thermal plasma reduces periodontitis-induced alveolar bone loss in rats[J]. Biochem Biophys Res Commun, 2018, 503(3): 2040-2046.
[29] Küçük D, Savran L, Ercan UK, et al. Evaluation of efficacy of non-thermal atmospheric pressure plasma in treatment of periodontitis: a randomized controlled clinical trial[J]. Clin Oral Investig, 2020, 24(9): 3133-3145.
[30] 宋应亮, 张思佳. 对种植体周围炎的认识与预防[J]. 华西口腔医学杂志, 2020, 38(5): 479-483.
Song YL, Zhang SJ. Insights into peri-implantitis and its prevention[J]. West China J Stomatol, 2020, 38(5): 479-483.
[31] Yang Y, Zheng M, Yang Y, et al. Inhibition of bacterial growth on zirconia abutment with a helium cold atmospheric plasma jet treatment[J]. Clin Oral Investig, 2020, 24(4): 1465-1477.
[32] Alqutaibi AY, Aljohani A, Alduri A, et al. The effectiveness of cold atmospheric plasma (CAP) on bacterial reduction in dental implants: a systematic review[J]. Biomolecules, 2023, 13(10): 1528.
[33] Carreiro AFP, Delben JA, Guedes S, et al. Low-temperature plasma on peri-implant-related biofilm and gingival tissue[J]. J Periodontol, 2019, 90(5): 507-515.
[34] Matthes R, Jablonowski L, Holtfreter B, et al. Fibroblast growth on zirconia ceramic and titanium disks after application with cold atmospheric pressure plasma devices or with antiseptics[J]. Int J Oral Maxillofac Implants, 2019, 34(4): 809-818.
[35] Wagner G, Eggers B, Duddeck D, et al. Influence of cold atmospheric plasma on dental implant mate-rials-an in vitro analysis[J]. Clin Oral Investig, 2022, 26(3): 2949-2963.
[36] Zheng Z, Ao XG, Xie P, et al. Nonthermal plasma brush treatment on titanium and zirconia to improve periabutment epithelium formation[J]. ACS Biomater Sci Eng, 2021, 7(11): 5039-5047.
[37] Canullo L, Rakic M, Corvino E, et al. Effect of argon plasma pre-treatment of healing abutments on peri-implant microbiome and soft tissue integration: a proof-of-concept randomized study[J]. BMC Oral Health, 2023, 23(1): 27.
[38] Zheng Z, He YJ, Long L, et al. Involvement of PI3K/Akt signaling pathway in promoting osteoge-nesis on titanium implant surfaces modified with no-vel non-thermal atmospheric plasma[J]. Front Bioeng Biotechnol, 2022, 10: 975840.
[39] Jang MH, Park YB, Kwon JS, et al. Osseointegration of plasma jet treated titanium implant surface in an animal model[J]. Materials, 2021, 14(8): 1942.
[40] Long L, Zhang M, Gan SQ, et al. Comparison of early osseointegration of non-thermal atmospheric plasma-functionalized/SLActive titanium implant surfaces in beagle dogs[J]. Front Bioeng Biotechnol, 2022, 10: 965248.
[41] Liu JR, Xu GM, Shi XM, et al. Low temperature plasma promoting fibroblast proliferation by activa-ting the NF-κB pathway and increasing cyclinD1 expression[J]. Sci Rep, 2017, 7(1): 11698.
[42] Eggers B, Stope MB, Marciniak J, et al. Non-invasive physical plasma generated by a medical argon plasma device induces the expression of regenerative factors in human gingival keratinocytes, fibroblasts, and tissue biopsies[J]. Biomedicines, 2022, 10(4): 889.
[43] Pekbağrıyanık T, Dadas FK, Enhoş Ş. Effects of non-thermal atmospheric pressure plasma on palatal wound healing of free gingival grafts: a randomized controlled clinical trial[J]. Clin Oral Investig, 2021, 25(11): 6269-6278.
[44] Ibáñez-Mancera NG, López-Callejas R, Toral-Rizo VH, et al. Cold atmospheric plasma benefits after a biopsy of the gingiva and palate: a case series[J]. Plasma Med, 2022, 12(4): 1-9.
[45] Kusakci-Seker B, Demirayak-Akdemir M. The effect of non-thermal atmospheric pressure plasma application on wound healing after gingivectomy[J]. Int Wound J, 2020, 17(5): 1376-1383.
[46] Ibáñez-Mancera NG, López-Callejas R, Toral-Rizo VH, et al. Healing of recurrent aphthous stomatitis by non-thermal plasma: pilot study[J]. Biomedicines, 2023, 11(1): 167.
[47] Haghighi L, Azizi A, Vatanpour M, et al. Antibacterial efficacy of cold atmospheric plasma, photodynamic therapy with two photosensitizers, and diode laser on primary mandibular second molar root canals infected with Enterococcus faecalis: an in vitro study[J]. Int J Dent, 2023, 2023: 5514829.
[48] Arguello-Sánchez R, López-Callejas R, Rodríguez-Méndez BG, et al. Innovative curved-tip reactor for non-thermal plasma and plasma-treated water gene-ration: synergistic impact comparison with sodium hypochlorite in dental root canal disinfection[J]. Materials, 2023, 16(22): 7204.
[49] Strazzi-Sahyon HB, Suzuki TYU, Lima GQ, et al. In vitro study on how cold plasma affects dentin surface characteristics[J]. J Mech Behav Biomed Mater, 2021, 123: 104762.
[50] Mousavi SFP, Ganjovi A, Eskandarizadeh A, et al. Effects of nonthermal atmospheric pressure plasma jet on human dental pulp stem cells[J]. Plasma Med, 2021, 11(3): 41-58.
[51] 毛学理, 施松涛. 牙髓干细胞与牙髓再生[J]. 中华口腔医学研究杂志(电子版), 2022, 16(6): 333-342.
Mao XL, Shi ST. Dental pulp stem cells and pulp regeneration[J]. Chin J Stomatol Res Electron Ed, 2022, 16(6): 333-342.
[52] Yoo YJ, Kang MJ, Perinpanayagam H, et al. Non-thermal atmospheric pressure plasma-conditioned root dentin promotes attraction and attachment of primary human dental pulp stem cells in real-time ex vivo [J]. Appl Sci, 2021, 11(15): 6836.
[53] Sonoda S, Mei YF, Atsuta I, et al. Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells[J]. Sci Rep, 2018, 8(1): 3419.
[54] Tominami K, Kanetaka H, Sasaki S, et al. Cold atmospheric plasma enhances osteoblast differentiation[J]. PLoS One, 2017, 12(7): e0180507.
[55] Eggers B, Marciniak J, Memmert S, et al. The beneficial effect of cold atmospheric plasma on parameters of molecules and cell function involved in wound healing in human osteoblast-like cells in vitro [J]. Odontology, 2020, 108(4): 607-616.
[56] Hartwig S, Preissner S, Voss JO, et al. The feasibility of cold atmospheric plasma in the treatment of complicated wounds in cranio-maxillo-facial surgery[J]. J Craniomaxillofac Surg, 2017, 45(10): 1724-1730.
[57] Rutkowski R, Daeschlein G, von Woedtke T, et al. Long-term risk assessment for medical application of cold atmospheric pressure plasma[J]. Diagnostics (Basel), 2020, 10(4): 210.
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