Int J Stomatol

Inter J Stomatol ›› 2018, Vol. 45 ›› Issue (2): 170-176.doi: 10.7518/gjkq.2018.02.009

• Original Articles • Previous Articles     Next Articles

Establishment of diabetic mouse model with conditional knockout of FoxO1 in osteoblasts

Xiong Yi, Gong Ping, Wu Yingying   

  1. State Key Laboratory of Oral Diseases &National Clinical Research Center for Oral Diseases &Dept. of Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2017-09-15 Revised:2017-12-20 Online:2018-03-01 Published:2018-03-01
  • Supported by:
    This study was supported by National Natural Science Foundation of China (81400543, 81571008) and Outstanding Scholars Research Funding of Sichuan University (2017SCU04A21).

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Abstract: Objective This study aims to establish a diabetic mouse model with conditional knockout of FoxO1 in osteoblasts and preliminary explore the phenotypes.Methods Wild type (WT) and gene knockout mice were obtained according to the designed reproductive strategy. DNA was extracted from mouse tail and amplified by real-time polymerase chain reaction (RT-PCR). The mice phenotypes were also detected. The mRNA and protein levels of FoxO1 in osteoblasts and other tissues were obtained and detected by RT-PCR and Western blot analysis. Mice weight and fasting blood glucose were measured. Insulin tolerance test (ITT) was conducted to assess the role of FoxO1 in glucose metabolism. Results Diabetic mouse models with conditional knockout of FoxO1 in osteoblasts were successfully established. The body weight of diabetic mice was significantly lower than that of normal mice at 2 weeks. The fasting blood glucose in diabetic gene knockout mice was lower than that in diabetic WT mice. In addition, ITT showed that FoxO1 knockout promoted insulin sensitivity in diabetic gene knockout mice compared with that in diabetic WT mice.Conclusion Diabetic mouse model with conditional knockout of FoxO1 in osteoblasts was successfully established. FoxO1 knockout ameliorated hyperglycaemia, which may be accounted for the increased insulin sensitivity in diabetic gene knockout mice.

Key words: forkhead box protein O, conditional knock-out, diabetes mellitus

CLC Number: 

  • R78

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