Int J Stomatol

Inter J Stomatol ›› 2015, Vol. 42 ›› Issue (2): 166-169.doi: 10.7518/gjkq.2015.02.011

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Arecoline upregulated matrix metalloproteinase 9 expression in HaCaT cells

Li Ming1,2, Peng Jieying2, Wu Yingfang2, Zhang Rui1   

  1. 1. Changsha Stomatological Hospital, Changsha 410005, China; 2. Stomatological Center, Xiangya Hospital, Central South University, Changsha 410078, China
  • Received:2014-07-12 Revised:2014-11-11 Online:2015-03-01 Published:2015-03-01

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Abstract:

Objective This study was designed to investigate the mechanism of arecoline-induced matrix metalloproteinase 9(MMP9) expression in epithelial HaCaT cells. Methods HaCaT cells were treated with arecoline having different concentrations at different times. MMP9, nuclear factor(NF)-κB, activator protein(AP)-1, and Ets-1 protein expressions were detected by western blot analysis. Transcription factors of regulated MMP-9 expression induced by arecoline were detected by RNA interference test. Results Arecoline upregulated MMP9 expression in HaCatcells through the modes of dosage and time symbiosis. Furthermore, arecoline upregulated NF-κB, AP-1, and Ets-1 protein expressions. As detected by the siRNA test, arecoline affected MMP9 through transcription factors, such as NF-κB, AP-1, and Ets-1, especially NF-κB. Conclusion Arecoline can upregulate MMP9 expression by promoting NF-κB, AP-1, and Ets-1 expressions and primarily through NF-κB transcription.

Key words: arecoline, epithelial cell, matrix metalloproteinase 9


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