国际口腔医学杂志

国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (4): 414-419.doi: 10.7518/gjkq.2018.04.008

• 综述 • 上一篇    下一篇

海藻酸-壳聚糖微球的制备及其在生物医药领域的应用

金鑫1, 杨军星1, 王英男2, 刘志辉1, 王博蔚3   

  1. 1.吉林大学口腔医院修复科 长春 130021;
    2.吉林大学化学学院 长春 130021;
    3.吉林大学第二医院妇产科 长春 130041
  • 收稿日期:2017-12-21 修回日期:2018-04-18 出版日期:2018-07-02 发布日期:2018-07-02
  • 通讯作者: 刘志辉,副教授,博士,Email：liuzhihui1975@sina.com
  • 作者简介:金鑫,住院医师,硕士,Email：441266577@qq.com
  • 基金资助:
    吉林省医药产业推进计划（20140311088YY）; 长春市重大科技攻关计划（2014054）; 吉林省科技攻关计划（20150204004YY）; 长春市“双十工程”计划（16SS12）

Preparation and biomedical applications of alginate-chitosan microspheres

Jin Xin1, Yang Junxing1, Wang Yingnan2, Liu Zhihui1, Wang Bowei3   

  1. 1. Dept. of Prosthodontics, Hospital of Stomatology, Jilin University, Changchun 130021, China;
    2. College of Chemistry, Jilin University, Changchun 130021, China;
    3. Dept. of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun 130041, China
  • Received:2017-12-21 Revised:2018-04-18 Online:2018-07-02 Published:2018-07-02
  • Supported by:
    This study was supported by Pharmaceutical Industry Promotion Plan of Jilin Province (20140311088YY), Key Scientific and Technological Projects of Changchun City (2014054), Technology Research Program of Jilin Province (20150204004YY) and “Double Ten” Plan of Changchun City (16SS12).

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摘要： 壳聚糖是唯一的天然碱性氨基多糖,海藻酸是一种天然阴离子多聚糖,这两种物质都具有良好的生物相容性,无细胞毒性,具有生物可降解性,是制备微球的良好材料。本文综合阐述了近年来国内外联合利用壳聚糖和海藻酸两种天然高分子物质制备微球的主要方法（包括离子交联法和乳化法）,以及海藻酸-壳聚糖微球作为药物载体在生物医药领域的应用。

关键词: 壳聚糖, 海藻酸, 载药, 控释, 微球

Abstract: Chitosan is the only natural alkaline amino polysaccharide, and alginate is a kind of natural anionic polysac-charide. These polysaccharides are good materials for microsphere preparation because of their good biocompatibility, nontoxicity and biodegradability. This paper reviews the main methods (ion crosslinking and emulsification) of microsphere preparation by using the combination of chitosan and alginate and its application as a drug carrier in biomedicine.

Key words: chitosan, alginate, drug-loaded, control release, microsphere

中图分类号: 

  • R318.08
[1] Croisier F, Jérôme C.Chitosan-based biomaterials for tissue engineering[J]. Euro Polymer J, 2013, 49(4): 780-792.
[2] Dash M, Chiellini F, Ottenbrite RM, et al.Chi-tosan—a versatile semi-synthetic polymer in biome-dical applications[J]. Prog Polymer Sci, 2011, 36(8): 981-1014.
[3] Jayakumar R, Menon D, Manzoor K, et al.Biome-dical applications of chitin and chitosan based nano-materials—a short review[J]. Carbohy Polymer, 2010, 82(2): 227-232.
[4] 李洁, 朱红娜, 周莉莉, 等. 海藻酸盐-壳聚糖复合微/纳米凝胶研究进展[J]. 化学研究, 2013, 24(2): 207-211.Li J, Zhu HN, Zhou LL, et al.Research progress on composite micro/nano-gels prepared from alginate and chitosan[J]. Chem Res, 2013, 24(2): 207-211.
[5] 杨子明, 彭政, 周敏, 等. 壳聚糖靶向缓释功能高分子载药微球及其特性研究[J]. 功能材料, 2013, 44(12): 1703-1708.Yang ZM, Peng Z, Zhou M, et al.Drug-loading chitosan polymer microsphere with targeted and slow-release function and its characteristics[J]. J Func Mater, 2013, 44(12): 1703-1708.
[6] Anitha A, Sowmya S, Kumar PTS, et al.Chitin and chitosan in selected biomedical applications[J]. Prog Polymer Sci, 2014, 39(9): 1644-1667.
[7] Hu L, Sun Y, Wu Y.Advances in chitosan-based drug delivery vehicles[J]. Nanoscale, 2013, 5(8): 3103-3111.
[8] 蒋挺大. 壳聚糖[M]. 北京: 化学工业出版社, 2001: 44-47.Jiang TD.Chitosan[M]. Beijing: Chemical Industry Press, 2001: 44-47.
[9] Gao C.Preparation of sodium alginate hydrogel and its application in drug release[J]. Prog Chem, 2013, 25(6): 1012-1022.
[10] Zou X, Zhao X, Ye L, et al.Preparation and drug release behavior of pH-responsive bovine serum albumin-loaded chitosan microspheres[J]. J Ind Eng Chem, 2015, 21: 1389-1397.
[11] 杨军星, 王琦, 王媛媛, 等. 双重载药多层海藻酸盐- 壳聚糖缓释微球的制备及体外释放[J]. 高等学校化学学报, 2015, 36(5): 1025-1032.Yang JX, Wang Q, Wang YY, et al.Preparation and in vitro release of multilayer alginate chitosan micro-spheres loading VEGF and vancomycin[J]. Chem J Chin Univ, 2015, 36(5): 1025-1032.
[12] 付昱, 杨敏, 李妍, 等. 基于天然高分子基元的阻隔层对磁性载药聚乳酸微球的控释作用[J]. 高等学校化学学报, 2012, 33(12): 2779-2783.Fu Y, Yang M, Li Y, et al.Effect of layer-by-layer films of natural polymers on drug release of magne-tic poly(lactic acid) microspheres[J]. Chem J Chin Univ, 2012, 33(12): 2779-2783.
[13] Li X, Kong X, Shi S, et al.Preparation of alginate coated chitosan microparticles for vaccine delivery[J]. BMC Biotech, 2008, 8(1): 89.
[14] Janes KA, Calvo P, Alonso MJ.Polysaccharide colloidal particles as delivery systems for macro-molecules[J]. Adv Drug Deliv Rev, 2001, 47(1): 83-97.
[15] Shu XZ, Zhu KJ.A novel approach to prepare tri-polyphosphate/chitosan complex beads for controlled release drug delivery[J]. Int J Pharm, 2000, 201(1): 51-58.
[16] 陈丽媛, 党奇嶂, 刘成圣, 等. 壳聚糖-固态分散体载药微球的制备及性能研究[J]. 功能材料, 2012, 43(13): 1762-1765.Chen LY, Dang QZ, Liu CS, et al.Preparation and performance study of chitosan microspheres loaded solid dispersion[J]. J Func Mater, 2012, 43(13): 1762-1765.
[17] 迟雪, 张丹丹. 星点设计-效应面法优化石榴皮提取物微球制备工艺研究[J]. 林产化学与工业, 2015, 35(1): 133-137.Chi X, Zhang DD.Optimized preparation of pome-granate peel extracts microspheres using central composite design and response surface method[J]. Chem Ind Forest Produc, 2015, 35(1): 133-137.
[18] 王婷婷, 曹光群, 董伟. 响应面法优化海藻酸钠微球包封L-抗坏血酸的制备工艺[J]. 日用化学工业, 2014, 44(8): 443-447.Wang TT, Cao GQ, Dong W.Optimization of the process for preparation of sodium alginate micro-spheres encapsulating L-ascorbic acid by response surface method[J]. Chin Surfac Deterg Cosmet, 2014, 44(8): 443-447.
[19] 张旭, 顾志鹏, 徐源廷, 等. 氧化海藻酸钠交联壳聚糖载药复合体系的制备及其药物缓释初步研究[J]. 功能材料, 2011, 42(5): 894-896.Zhang X, Gu ZP, Xu YT, et al.Preparation of oxidized alginate sodium cross-linked chitosan scaf-fold for drug delivery[J]. J Func Mater, 2011, 42(5): 894-896.
[20] Mao Y, Zhao M, Ge Y, et al.Novel alginate-chitosan composite microspheres for implant delivery of vancomycin and in vivo evaluation[J]. Chem Biol Drug Des, 2016, 88(3): 434-440.
[21] Chen H, Xing X, Tan H, et al.Covalently antibac-terial alginate-chitosan hydrogel dressing integrated gelatin microspheres containing tetracycline hydro-chloride for wound healing[J]. Mater Sci Eng C Mater Biol Appl, 2017, 70(Pt 1): 287-295.
[22] Shi X, Fang Q, Ding M, et al.Microspheres of car-boxymethyl chitosan, sodium alginate and collagen for a novel hemostatic in vitro study[J]. J Biomater Appl, 2016, 30(7): 1092-1102.
[23] Rodness J, Mihic A, Miyagi Y, et al.VEGF-loaded microsphere patch for local protein delivery to the ischemic heart[J]. Acta Biomater, 2016, 45: 169-181.
[24] Fletcher NA, Babcock LR, Murray EA, et al.Con-trolled delivery of antibodies from injectable hydro-gels[J]. Mater Sci Eng C Mater Biol Appl, 2016, 59: 801-806.
[25] Duan H, Lü S, Qin H, et al.Co-delivery of zinc and 5-aminosalicylic acid from alginate/N-succinyl-chi-tosan blend microspheres for synergistic therapy of colitis[J]. Int J Pharm, 2017, 516(1/2): 214-224.
[26] Wang QS, Wang GF, Zhou J, et al.Colon targeted oral drug delivery system based on alginate-chitosan microspheres loaded with icariin in the treatment of ulcerative colitis[J]. Int J Pharm, 2016, 515(1/2): 176-185.
[27] Yalcin A, Soddu E, Turunc Bayrakdar E, et al.Neu-roprotective effects of engineered polymeric nasal microspheres containing hydroxypropyl-β-cyclo-dextrin on β-amyloid (1-42)-induced toxicity[J]. J Pharm Sci, 2016, 105(8): 2372-2380.
[28] Hazra M, Dasgupta Mandal D, Mandal T, et al.Designing polymeric microparticulate drug delivery system for hydrophobic drug quercetin[J]. Saudi Pharm J, 2015, 23(4): 429-436.
[29] Jelvehgari M, Barghi L, Barghi F.Preparation of chlorpheniramine maleate-loaded alginate/chitosan particulate systems by the ionic gelation method for taste masking[J]. Jundishapur J Nat Pharm Prod, 2014, 9(1): 39-48.
[30] Wang QS, Zhu XN, Jiang HL, et al.Protective effects of alginate-chitosan microspheres loaded with al-kaloids from Coptis chinensis Franch. and Evodia rutaecarpa (Juss.) Benth. (Zuojin Pill) against ethanol-induced acute gastric mucosal injury in rats[J]. Drug Des Devel Ther, 2015, 9: 6151-6165.
[31] Liu Q, Rauth AM, Liu J, et al.Characterization of a microsphere formulation containing glucose oxidase and its in vivo efficacy in a murine solid tumor model[J]. Pharm Res, 2009, 26(10): 2343-2357.
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