国际口腔医学杂志

国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (3): 255-260.doi: 10.7518/gjkq.2018.03.002

• 干细胞专栏 • 上一篇    下一篇

体外培养环境影响牙周膜干细胞生物学特性的研究进展

贾凌璐1,2, 文勇1,2, 徐欣1,2   

  1. 1.山东大学口腔医学院 济南 250012;
    2.山东省口腔组织再生重点实验室 济南 250012
  • 收稿日期:2017-09-28 修回日期:2018-01-15 出版日期:2018-05-01 发布日期:2018-05-01
  • 通讯作者: 文勇,副教授,博士,Email：wenyong@sdu.edu.cn
  • 作者简介:贾凌璐,博士,Email：danlingjia@163.com
  • 基金资助:
    山东省重点研发计划（2016GSF201115,2017GSF181- 17）;中国博士后基金 （2017M610432）;山东省医药卫生发展计划（2017WS111）

Effects of culture conditions in vitro on the biological characteristics of periodontal ligament stem cells

Jia Linglu1,2, Wen Yong1,2, Xu Xin1,2   

  1. 1. School of Stomatology, Shandong University, Jinan 250012, China;
    2. Shandong Province Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China
  • Received:2017-09-28 Revised:2018-01-15 Online:2018-05-01 Published:2018-05-01
  • Supported by:
    This study was supported by Shandong Provincial Key Research and Development Program (2016GSF201115, 2017GSF18117), China Postdoctoral Science Foundation (2017M610432) and Shandong Pharmaceutical and Health Science Technology Development Programme (2017WS111).

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摘要： 牙周膜干细胞是具有自我更新能力与多向分化潜能的成体干细胞,作为口腔组织工程研究的候选种子细胞备受关注。进行体外培养过程中,原代细胞分离方法、基础培养基成分、氧气浓度、培养时间、细胞保存条件及其他物理和化学刺激的施加等培养因素可在一定程度上改变牙周膜干细胞的增殖、分化、迁移等特性,将影响其在组织工程中应用的效果。本文围绕体外培养环境对牙周膜干细胞生物学特性的影响进行综述,为优化牙周膜干细胞体外培养条件、充分发挥其全能性提供参考。

关键词: 牙周膜干细胞, 细胞培养技术, 生物学特性

Abstract: Periodontal ligament stem cell, which is a kind of adult stem cell, owns the abilities of self-renewing and multi-differentiation and has become the hot spot in the oral tissue engineering. When cultured in vitro, different culture conditions such as primary cell separation methods, culture medium composition, oxygen concentration, culture time, cell storage conditions and other physical or chemical stimulation can change the proliferation, immigration or differentiation characteristics of periodontal ligament stem cell. This paper is committed to review on the research progress regarding the effects of the culture conditions in vitro on the biological characteristics of periodontal ligament stem cell in recent years and provide theoretical reference for tissue engineering.

Key words: periodontal ligament stem cell, cell culture technique, biological characteristics

中图分类号: 

  • Q813.1+1
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