国际口腔医学杂志

国际口腔医学杂志 ›› 2017, Vol. 44 ›› Issue (2): 204-208.doi: 10.7518/gjkq.2017.02.018

• 综述 • 上一篇    下一篇

牙周韧带细胞膜片技术用于牙周组织再生的研究进展

姜雨汐1, 2, 刘树泰1, 2   

  1. 1.滨州医学院口腔医学院 烟台 264000;
    2.烟台市口腔医院牙周科 烟台 264000
  • 收稿日期:2016-01-31 出版日期:2017-03-01 发布日期:2017-03-01
  • 通讯作者: 刘树泰,副教授,博士,Email：dentistliu@163.com
  • 作者简介:姜雨汐,硕士,Email：dentistjyx@163.com

Advances in periodontal regeneration with periodontal ligament cell sheet technique

Jiang Yuxi, Liu Shutai   

  1. 1. School of Stomatology, Binzhou Medical University, Yantai 264000, China;
    2. Dept. of Periodontics, Yantai Stomatological Hospital, Yantai 264000, China
  • Received:2016-01-31 Online:2017-03-01 Published:2017-03-01

RichHTML

11

PDF (PC)

579

被引次数

4

摘要： 为了解决中重度牙周炎引发的牙周组织破坏后无法自主再生的问题,细胞膜片工程根据引导性组织再生术的基本原理,通过将使用温敏培养皿等方法获取的完整细胞片层直接移植贴附到缺损部位的方法,实现获取牙骨质、牙槽骨及嵌入二者的牙周韧带同时再生的目标。近年来,细胞膜片技术不断发展,逐步克服了单层细胞膜片厚度偏低、血管化不良及自体细胞来源不足等问题,其中运用牙周韧带细胞膜片进行牙周组织再生已经正式进入临床试验阶段。本文对近年来牙周韧带细胞膜片及其他常用细胞膜片在牙周组织再生方向应用的新进展作一综述。

关键词: 细胞膜片技术, 牙周韧带细胞, UpCell培养皿, 去细胞化, 牙周组织再生

Abstract: Achieving complete periodontal tissue regeneration that arises from periodontitis show some difficulties. The principles of guided tissue regeneration indicate that cell sheet engineering aims to achieve the regeneration of periodontal ligament embedded in new cementum and new alveolar bone by several novel cell-transferring Methods. For example, UpCell culture dish can control cell-surface adhesion and utilize temperature change in cell culture and a surface-grafted temperature-sensitive polymer named poly N-isopropylacylamide. Cell sheets have been harvested from temperature-sensitive culture surfaces non-invasively and overlaid as multilayered constructs. Cell sheet technique has improved in recent years and has overcome many obstacles, such as the inadequate thickness of monolayer cell sheet, the insufficient vascularization, and the lack of autologous cell sources. Owing to the high metabolic turnover rates of the periodontal ligament, many researchers have considered periodontal ligament cells as one of the most suitable candidate cell sources for regeneration. A clinical study of periodontal regeneration with autologous periodontal ligament cell sheet has been recently performed on humans in Japan. This article reviews the recent advances of techniques in periodontal ligament cell sheet engineering and its application in periodontal regeneration.

Key words: cell sheet technique, periodontal ligament cell, UpCell culture dish, decellularization, periodontal regeneration

中图分类号: 

  • Q813.1
[1] Bartold PM, Gronthos S, Ivanovski S, et al. Tissue engineered periodontal products[J]. J Periodont Res, 2016, 51(1):1-15.
[2] Matsuura K, Utoh R, Nagase K, et al. Cell sheet approach for tissue engineering and regenerative medicine[J]. J Control Release, 2014, 190:228-239.
[3] Iwata T, Washio K, Yoshida T, et al. Cell sheet en-gineering and its application for periodontal rege-neration[J]. J Tissue Eng Regen Med, 2015, 9(4): 343-356.
[4] Kim CS, Um YJ, Chai JK, et al. A canine model for histometric evaluation of periodontal regeneration[J]. Periodontol 2000, 2011, 56(1):209-226.
[5] Jönsson D, Nebel D, Bratthall G, et al. The human periodontal ligament cell: a fibroblast-like cell acting as an immune cell[J]. J Periodont Res, 2011, 46(2): 153-157.
[6] Dan H, Vaquette C, Fisher AG, et al. The influence of cellular source on periodontal regeneration using calcium phosphate coated polycaprolactone scaffold supported cell sheets[J]. Biomaterials, 2014, 35(1): 113-122.
[7] Tsumanuma Y, Iwata T, Washio K, et al. Com-parison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model[J]. Biomaterials, 2011, 32(25):5819-5825.
[8] Vaquette C, Fan W, Xiao Y, et al. A biphasic scaf-fold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/perio-dontal ligament complex[J]. Biomaterials, 2012, 33 (22):5560-5573.
[9] Costa PF, Vaquette C, Zhang Q, et al. Advanced tissue engineering scaffold design for regeneration of the complex hierarchical periodontal structure[J]. J Clin Periodontol, 2014, 41(3):283-294.
[10] Nagai N, Hirakawa A, Otani N, et al. Development of tissue-engineered human periodontal ligament constructs with intrinsic angiogenic potential[J]. Cells Tissues Organs, 2009, 190(6):303-312.
[11] Haraguchi Y, Shimizu T, Sasagawa T, et al. Fabrica-tion of functional three-dimensional tissues by stac-king cell sheets in vitro [J]. Nat Protoc, 2012, 7(5): 850-858.
[12] Farag A, Vaquette C, Theodoropoulos C, et al. De-cellularized periodontal ligament cell sheets with recellularization potential[J]. J Dent Res, 2014, 93 (12):1313-1319.
[13] Wei F, Qu C, Song T, et al. Vitamin C treatment promotes mesenchymal stem cell sheet formation and tissue regeneration by elevating telomerase activity[J]. J Cell Physiol, 2012, 227(9):3216-3224.
[14] Wei F, Song T, Ding G, et al. Functional tooth re-storation by allogeneic mesenchymal stem cell-based bio-root regeneration in swine[J]. Stem Cells Dev, 2013, 22(12):1752-1762.
[15] Zhao YH, Zhang M, Liu NX, et al. The combined use of cell sheet fragments of periodontal ligament stem cells and platelet-rich fibrin granules for avu-lsed tooth reimplantation[J]. Biomaterials, 2013, 34 (22):5506-5520.
[16] Yan XZ, Both SK, Yang PS, et al. Human perio-dontal ligament derived progenitor cells: effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior[J]. Biomed Res Int, 2014, 2014:145423.
[17] Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement[J]. Cytotherapy, 2006, 8(4):315-317.
[18] Izumi Y, Aoki A, Yamada Y, et al. Current and fu-ture periodontal tissue engineering[J]. Periodontol 2000, 2011, 56(1):166-187.
[19] Iwata T, Yamato M, Zhang Z, et al. Validation of human periodontal ligament-derived cells as a re-liable source for cytotherapeutic use[J]. J Clin Perio-dontol, 2010, 37(12):1088-1099.
[20] Guo S, Guo W, Ding Y, et al. Comparative study of human dental follicle cell sheets and periodontal ligament cell sheets for periodontal tissue regenera-tion[J]. Cell Transplant, 2013, 22(6):1061-1073.
[21] Okuda K, Yamamiya K, Kawase T, et al. Treatment of human infrabony periodontal defects by grafting human cultured periosteum sheets combined with platelet-rich plasma and porous hydroxyapatite granules: case series[J]. J Int Acad Periodontol, 2009, 11(3):206-213.
[22] Murakami S. Periodontal tissue regeneration by signaling molecule(s): what role does basic fibroblast growth factor(FGF-2) have in periodontal therapy [J]. Periodontol 2000, 2011, 56(1):188-208.
[23] Yoshida T, Washio K, Iwata T, et al. Current status and future development of cell transplantation the-rapy for periodontal tissue regeneration[J]. Int J Dent, 2012, 2012:307024.
[24] Wang J, Zhang R, Shen Y, et al. Recent advances in cell sheet technology for periodontal regeneration[J]. Curr Stem Cell Res Ther, 2014, 9(3):162-173.
[1] 范琳,孙江. 微针在口腔医学中的应用[J]. 国际口腔医学杂志, 2023, 50(4): 472-478.
[2] 贾婷婷,颜世果. 特异性AT序列结合蛋白2在颌面部发育及牙周组织再生中作用的研究进展[J]. 国际口腔医学杂志, 2019, 46(3): 320-325.
[3] 董正谋,刘锐,刘鲁川,温秀杰. 种子细胞在牙周组织再生治疗中的研究进展[J]. 国际口腔医学杂志, 2019, 46(1): 48-54.
[4] 李昕怡，董伟. 釉基质蛋白促进牙周组织再生的研究进展[J]. 国际口腔医学杂志, 2015, 42(5): 600-605.
[5] 鲁少文 税艳青. 牙周膜干细胞的研究进展[J]. 国际口腔医学杂志, 2013, 40(6): 769-772.
[6] 张静 李纾. 控释技术在牙周组织再生治疗中的应用[J]. 国际口腔医学杂志, 2013, 40(5): 616-618.
[7] 项陈洋1综述 张凌琳2，3 李伟2审校. 釉基质蛋白在口腔医学领域中的应用进展[J]. 国际口腔医学杂志, 2012, 39(6): 766-769.
[8] 王姹综述 徐燕审校. 生长因子在牙周组织再生中的有效释放方式[J]. 国际口腔医学杂志, 2012, 39(2): 265-268.
[9] 唐焜琪综述 闫福华审校. 骨保护蛋白在牙周组织再生中的应用[J]. 国际口腔医学杂志, 2011, 38(5): 546-549.
[10] 孙静综述 李纾审校. 牙周膜干细胞巢与牙周组织再生[J]. 国际口腔医学杂志, 2011, 38(4): 460-462.
[11] 孔宁静1综述刘建国1 李厚轩2 闫福华2审校. 脂多糖对牙周膜细胞生物学性状的影响[J]. 国际口腔医学杂志, 2011, 38(3): 370-372.
[12] 黄晶1 宋爱梅1，2综述 杨丕山1，2审校. 细胞层片移植的研究现状[J]. 国际口腔医学杂志, 2011, 38(2): 221-224.
[13] 陈芳综述 徐燕审校. 牙周膜干细胞的研究进展[J]. 国际口腔医学杂志, 2008, 35(6): 672-672～674.
[14] 丁佩惠,陈莉丽,. 基因治疗在牙周组织再生中的应用[J]. 国际口腔医学杂志, 2007, 34(02): 97-99.
[15] 刘琼,谢昊. 牙周韧带细胞与成体干细胞[J]. 国际口腔医学杂志, 2005, 32(06): 468-470.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 张京剧. 青年期至中年期颅面复合体变化的头影测量研究[J]. 国际口腔医学杂志, 1999, 26(06): .
[2] 刘玲. 镍铬合金中铍对可铸造性和陶瓷金属结合力的影响[J]. 国际口腔医学杂志, 1999, 26(06): .
[3] 王昆润. 在种植体上制作固定义齿以后下颌骨密度的动态变化[J]. 国际口腔医学杂志, 1999, 26(06): .
[4] 王昆润. 重型颌面部炎症死亡和康复病例的实验室检查指标比较[J]. 国际口腔医学杂志, 1999, 26(06): .
[5] 逄键梁. 两例外胚层发育不良儿童骨内植入种植体后牙槽骨生长情况[J]. 国际口腔医学杂志, 1999, 26(05): .
[6] 温秀杰. 氟化物对牙本质脱矿抑制作用的体外实验研究[J]. 国际口腔医学杂志, 1999, 26(05): .
[7] 杨春惠. 耳颞神经在颞颌关节周围的分布[J]. 国际口腔医学杂志, 1999, 26(04): .
[8] 王昆润. 牙周炎加重期应选用何种抗生素[J]. 国际口腔医学杂志, 1999, 26(04): .
[9] 杨儒壮 孙宏晨 欧阳喈. 纳米级高分子支架材料在组织工程中的研究进展[J]. 国际口腔医学杂志, 2004, 31(02): 126 -128 .
[10] 严超然,李龙江. 肿瘤靶向药物载体系统的研究进展[J]. 国际口腔医学杂志, 2008, 35(S1): .