arginine,enamel,demineralization,remineralization,

几种牙膏对早期釉质龋再矿化效果的体外研究

国际口腔医学杂志 ›› 2012, Vol. 39 ›› Issue (6): 710-713.doi: 10.3969/j.issn.1673-5749.2012.06.002

• 论著 • 上一篇    下一篇

几种牙膏对早期釉质龋再矿化效果的体外研究

黄雅静 李月恒 黄锐 钱英子 段艳霞 周智   

  1. 重庆医科大学口腔医院牙体牙髓病科重庆401147
  • 收稿日期:2012-05-13 修回日期:2012-07-12 出版日期:2012-11-01 发布日期:2012-11-01
  • 通讯作者: 周智,Tel:023-88860065
  • 作者简介:黄雅静(1984—),女,河北人,硕士

An in vitro study of remineralization potential of several toothpastes on initial enamel lesions

Huang Yajing, Li Yueheng, Huang Rui, Qian Yingzi, Duan Yanxia, Zhou Zhi.   

  1. Dept. of Conservative Dentistry and Endodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China
  • Received:2012-05-13 Revised:2012-07-12 Online:2012-11-01 Published:2012-11-01

摘要:

目的比较几种不同成分牙膏促进脱矿釉质再矿化的作用。方法选择新鲜拔除的牛下颌切牙制备成釉质块,形成早期人工龋。随机分为5 组,各组依次为精氨酸牙膏组、氟离子浓度为0.11%牙膏组、酪磷酸蛋白牙膏组、氟化钠组(阳性对照组)、去离子水组(阴性对照组)。分别进行pH 循环后,试验样本依次用显微硬度仪、偏光显微镜、激光共聚焦镜对再矿化程度进行检测。运用SPSS 17.0 软件,对检测结果进行单因素方差分析及配对t检验分析。结果显微硬度检测结果显示:精氨酸牙膏组、氟化钠组及酪磷酸蛋白牙膏组比0.11%含氟牙膏组的显微硬度恢复的百分比高(P<0.05)。偏振光显微镜检测结果显示:酪磷酸蛋白牙膏组脱矿深度恢复的百分比高于0.11%含氟牙膏组,而较精氨酸牙膏组、氟化钠组低。激光共聚焦显微镜检测结果显示:酪磷酸蛋白牙膏组与0.11%含氟牙膏组荧光渗透减少量较精氨酸牙膏组及氟化钠组少。结论在本实验条件下,精氨酸牙膏、酪磷酸蛋白牙膏都能一定程度的促进早期釉质龋的再矿化作用,其中以精氨酸牙膏作用最强。

关键词: 精氨酸, 釉质, 脱矿, 再矿化

Abstract:

Objective To assess the remineralization property of several toothpastes on initial enamel lesions. Methods Bovine enamel blocks with in vitro produced initial lesion were used. The lesions were subjected to a pH-cycling regime for 12 d, with one of five treatments: NaF(positive control) group, pro-arginine group, casein phosphorylation of protein group, toothpaste with 0.11% fluoride group and with distilled and deionized water (DDW) group for negative control. The samples were subsequently evaluated using a microhardness tester, polarised light microscopy(PLM) and confocal laser scanning microscope(CLSM). Results Surface hardness measurements revealed pro-arginine group, casein phosphorylation of protein group and NaF group had significantly greater effect on enhancing remineralization than that of toothpaste with 0.11% fluoride group(P<0.05). Detailed investigation of PLM indicated that casein phosphorylation of protein group and toothpaste with 0.11% fluoride group had limited capacity to reduce the lesion depth compared with pro-arginine group and NaF group(P<0.05). Comparing with toothpaste with 0.11% fluoride group , casein phosphorylation of protein group had a significantly greater property of limiting the lesion depth(P<0.05). The results of CLSM inferred that the reduction of fluorescent dye infiltration in toothpaste with 0.11% fluoride group and casein phosphorylation of protein group was less than pro-arginine group and NaF group. Conclusion There is a significant synergistic effect of pro-arginine treatment on promoting the remineralization of initial enamel lesion compared with the other groups.

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