Int J Stomatol

Inter J Stomatol ›› 2018, Vol. 45 ›› Issue (3): 313-318.doi: 10.7518/gjkq.2018.03.013

• Original Articles • Previous Articles     Next Articles

Expression changes of platelet-derived growth factor receptor α in the process of palatal suspension culture of the C57BL/6J mouse in vitro

Cong Wenwen, Zhang Daizun, Xiao Wenlin, Xue Lingfa, Xu Yaoxiang   

  1. Stomatological Center, Affiliated Hospital of Qingdao University; Key Laboratory of Oral Clinical Medicine of Shandong Provincial Education Department, Qingdao 266555, China
  • Received:2017-05-31 Revised:2017-11-20 Published:2018-05-08
  • Supported by:
    This study was supported by Natural Science Foundation of Shandong Province (ZR2015HM022).

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Abstract: Objective This experiment builded in C57BL/6J mouse models of palatal process culture in vitro, to study the expression of platelet-derived growth factor receptor α (PDGFR-α) during different times. Methods Experiment cultured palates with the method of suspension culture for 24, 36, 48 h, palatal development was observed by stereomicroscope and hematoxylin-eosin (HE) staining. PDGFR-α expression location in different period was detected by immunohistochemical assays. The expression of PDGFR-α was examined by Western blot. Results Stereomicroscope and HE staining results showed that in vitro cultivation of 24 h, palatal process the space between bilateral palatal process reduced, bilateral palatal process contact and the crest epithelial seam could be seen in stereomicroscope and HE in 36 h, bilateral palatal process fusion and the crest epithelial seam disappearance could be seen in stereomicroscope and HE in 48 h. The expression level of PDGFR-α cultured palates for 24 h reached its peak and gradually reduced in 36 and 48 h. Conclusion The changes of PDGFR-α expression in cultivation of palatal process in vitro and in vivo had the same trend. In this study, improved method of suspension culture of palatal process in vitro simulated the development of the palatal process in vivo. The study had made a foundation for subsequent cleft palate of complex mechanismsd.

Key words: platelet-derived growth factor receptor α, palatal, suspension culture

CLC Number: 

  • Q813.1+2

TrendMD: 
[1] Setó-Salvia N, Stanier P.Genetics of cleft lip and/or cleft palate: association with other common anoma-lies[J]. Eur J Med Genet, 2014, 57(8):381-393.
[2] Dai L, Zhu J, Mao M, et al.Time trends in oral clefts in Chinese newborns: data from the Chinese National Birth Defects Monitoring Network[J]. Birth Defects Res A Clin Mol Teratol, 2010, 88(1):41-47.
[3] Velázquez-Aragón JA, Alcántara-Ortigoza MA, Estandia-Ortega B, et al.Gene interactions provide evidence for signaling pathways involved in cleft lip/palate in humans[J]. J Dent Res, 2016, 95(11): 1257-1264.
[4] Simioni M, Araujo TK, Monlleo IL, et al.Investiga-tion of genetic factors underlying typical orofacial clefts: mutational screening and copy number varia-tion[J]. J Hum Genet, 2015, 60(1):17-25.
[5] Soriano P.The PDGF alpha receptor is required for neural crest cell development and for normal patter-ning of the somites[J]. Development, 1997, 124(14): 2691-2700.
[6] Eberhart JK, He X, Swartz ME, et al.MicroRNA Mirn140 modulates Pdgf signaling during palato-genesis[J]. Nat Genet, 2008, 40(3):290-298.
[7] Stephenson DA, Mercola M, Anderson E, et al.Pla-telet-derived growth factor receptor alpha-subunit gene (Pdgfra) is deleted in the mouse patch (Ph) mu-tation[J]. Proc Natl Acad Sci USA, 1991, 88(1):6-10.
[8] 仵素珍, 康鹏, 肖文林, 等. 血小板衍生生长因子受体α基因在C57BL/6J小鼠胚胎腭突融合过程中表达变化的研究[J]. 口腔医学, 2015, 35(12):1008-1012.
Wu SZ, Kang P, Xiao WL, et al.The study of PDGF- α gene expression in the C57BL/6J mouse embryo palate shelves[J]. Stomatology, 2015, 35(12):1008-1012.
[9] Shiota K, Kosazuma T, Klug S, et al.Development of the fetal mouse palate in suspension organ culture[J]. Acta Anat (Basel), 1990, 137(1):59-64.
[10] 张岱尊, 庄翠竹, 肖文林, 等. C57BL/6J小鼠胚胎腭突体外培养模型的建立[J]. 上海口腔医学, 2013, 22(2):132-136.
Zhang DZ, Zhuang CZ, Xiao WL, et al.Establish-ment of palatal organ culture model of C57BL/6J mouse embryos in vitro[J]. Shanghai J Stomatol, 2013, 22(2):132-136.
[11] Abbott BD, Pratt RM.Human embryonic palatal epithelial differentiation is altered by retinoic acid and epidermal growth factor in organ culture[J]. J Craniofac Genet Dev Biol, 1987, 7(3):241-265.
[12] Brunet CL, Sharpe PM, Ferguson MW.The distribu-tion of epidermal growth factor binding sites in the developing mouse palate[J]. Int J Dev Biol, 1993, 37(3):451-458.
[13] Tiwari R, Bargmann W, Bose HR Jr.Activation of the TGF-beta/Smad signaling pathway in oncogenic transformation by v-Rel[J]. Virology, 2011, 413(1): 60-71.
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