Int J Stomatol

Inter J Stomatol ›› 2019, Vol. 46 ›› Issue (1): 12-19.doi: 10.7518/gjkq.2019.01.003

• Original Articles • Previous Articles     Next Articles

Animal research for the prognostic mechanism of traumatic temporomandibular joint ankyloses

Jun Guo1,Wei Fei1,Qinghua Li2()   

  1. 1. Dept. of Stomatology, Sichuan Provincial Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China;
    2. Dept. of Stomatology, The Affiliated Hospital of Panzhihua University, Panzhihua 617000, China
  • Received:2018-04-09 Revised:2018-10-12 Online:2019-01-01 Published:2019-01-11
  • Contact: Qinghua Li E-mail:153496777@qq.com
  • Supported by:
    This study was supported by Program for Sichuan Health and Family Planning Commission(18PJ096)

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Abstract:

Objective To evaluate the effect of different treatment methods on the prognosis of intracapsular condylar fracture based on the animal model of intracapsular condylar fracture in miniature pig, and research the mechanism of temporomandibular joint ankylosis. Methods Fifteen miniature pigs were randomly divided into 3 groups, 5 animals (10 joints) in each group. The bilateral intracapsular condylar fractures caused by adopting the method of operation with removing the lateral part of articular disc. The animals were treated with three kinds of treatment of intracapsular fractures. 6 months postoperative, the formation of temporomandibular joint ankylosis was evaluated by gross specimen observation, imaging and histological examination. The expression of vascular endothelial growth factor (VEGF), osteocalcin (OCN), osteopontin (OPN) and bone morphogenetic protein 2 (BMP2) in temporomandibular joint specimen were detected by immunohistochemical staining. The mRNA and protein expressions of VEGF, OCN, OPN and BMP2 in temporomandi-bular joint specimen were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western Blot respec-tively. Results The incidence of temporomandibular joint ankylosis in the experimental group was significantly lower than that in the control group (P<0.05). The mRNA and protein expression level of VEGF, OCN, OPN and BMP2 in the experi-mental group was significantly lower than that in the control group by RT-PCR and Western Blot (P<0.01). Conclusion Different treatment of intracapsular condylar fractures is closely related to the incidence of traumatic temporomandi-bular joint ankylosis. Removal of hematoma and reduction of bone wound exposure would be the key factors for prevention of temporomandibular joint ankylosis.

Key words: temporomandibular joint trauma, temporomandibular joint ankylosis, intracapsular condylar fracture

CLC Number: 

  • R782.6 +1

TrendMD: 

Fig 1

Establishment of animal model and experiment groups"

Tab 1

Changes in body weight before and 6 months after operation kg"

时间 自然愈合组 生理盐水组 钛板固定组
术前 21.00±0.01 14.20±0.04 14.20±0.05
术后 36.00±0.09 24.25±0.11 24.30±0.05

Tab 2

Maximum opening degree of each group before and 6 months after operation cm"

时间 自然愈合组 生理盐水组 钛板固定组
术前 6.90±0.16 6.80±0.16 6.84±0.30
术后 4.36±0.11 4.88±0.26* 5.42±0.36*

Fig 2

CT images were obtained 6 months after operation"

Fig 3

Living joint samples of control group of 6 months postoperatively"

Tab 3

Comparison of the incidence of temporomandibular joint ankylosis between experimental group and control group"

分组 样本量/侧 关节强直 强直率/% P值
有 无
自然愈合组 10 9 1 90
生理盐水组 10 4 6 40 0.029
钛板固定组 10 2 8 20 0.003

Fig 4

Histological observation of joints 6 months postoperatively"

Fig 5

Mean optical density value of VEGF, OCN, OPN and BMP2"

Fig 6

Expression of VEGF, OCN, OPN and BMP2 in each joint immunohistochemistry × 400"

Fig 7

mRNA of VEGF, OCN, OPN and BMP2 amplification status"

Fig 8

Relative protein expression value of VEGF, OCN, OPN and BMP2 through Western Blot"

Fig 9

VEGF, OCN, OPN and BMP2 express in each group through Western Blot"

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