Inter J Stomatol ›› 2018, Vol. 45 ›› Issue (5): 539-545.doi: 10.7518/gjkq.2018.05.008

• Original Articles • Previous Articles     Next Articles

Preparation of graphene oxide coatings on titanium alloy surface

Xueyang Deng,Lanlan Pan,Ting Hu,Wenhua Li,Xuerong. Xiang()   

  1. Dept. of Periodontology, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2017-10-19 Revised:2018-04-10 Online:2018-09-01 Published:2018-09-20
  • Contact: Xuerong. Xiang
  • Supported by:
    This study was supported by Pre-Research Project of National Natural Science Foundation of China in Chongqing Medical University(NSFYY20151- 6);Science and Technology Planning Project in Yubei District of Chongqing(2015 Social 10);Project Supported by Program for Innovation Team Building at Institutions of Higher Education in Chongqing(CXTDG201602006)


Objective To prepare a kind of coating on the titanium alloy surface with graphene oxide (GO) and to evaluate its biocompatibility preliminarily. Methods Ti substrates prepared by the alkali-hydrothermal reaction were coated with GO (Ti/GO) through layer-by-layer assembly of positively (GO-NH3 +) and negatively (GO-COO -) charged GO sheet with fifteen bilayers. The hydrophobic nature of GO coating on Ti substrates was analyzed by contact angle meter, and the microstructure of GO coating on Ti substrates were characterized by scanning electron microscope (SEM) and atomic force microscope (AFM). The in vitro cellular response was observed by the biological behaviors of bone marrow mesenchymal stem cells (BMSCs) which were inoculated on Ti substrates by SEM and fluorescence microscopy. Porphyromonas gingivalis was utilized to test the antibacterial effect of the Ti/GO surface. Results The GO coating with nano-scale surface topography on the aikali-hydrothermal reacted Ti alloy discs which was hydrophilic was prepared. The GO coating improved the adhension of BMSCs and kept cells live. 86.34% of bacteria had been killed on the Ti/GO surface. Conclusion The GO-coated Ti discs had good biocompatibility and antibacterial property.

Key words: graphene oxide, surface coating, biocom-patibility, antibacterial property

CLC Number: 

  • R783.1


Fig 1

Observations of Ti discs by naked eyes"

Fig 2

SEM images of Ti discs"

Fig 3

AFM images of Ti discs"

Fig 4

SEM images of BMSC cultured on Ti discs"

Fig 5

DAPI staining of BMSCs on Ti discs"

Fig 6

Live/dead staining of BMSCs cultured on Ti substrates for 24 h"

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