国际口腔医学杂志

国际口腔医学杂志 ›› 2024, Vol. 51 ›› Issue (6): 713-721.doi: 10.7518/gjkq.2024083

• 论著 • 上一篇    

不同脉冲能量Er: YAG激光对钛盘表面形貌及生物学性能的影响

刘翼1(),邱江珊2,申道南1,关鑫3,丁一1()   

  1. 1.口腔疾病防治全国重点实验室;国家口腔医学中心 国家口腔疾病临床医学研究中心;四川大学华西口腔医院牙周病科 成都 610041
    2.天津市口腔医院·南开大学口腔医院牙周病科 天津 300041
    3.口腔疾病防治全国重点实验室;国家口腔医学中心 国家口腔疾病临床医学研究中心;四川大学华西口腔医学院 成都 610041
  • 收稿日期:2024-03-05 修回日期:2024-06-20 出版日期:2024-11-01 发布日期:2024-11-04
  • 通讯作者: 丁一
  • 作者简介:刘翼，医师，博士，Email：liuyi66116161@163.com

Effect of Er: YAG laser with different pulse energies on the surface morphologies and biological properties of titanium discs

Yi Liu1(),Jiangshan Qiu2,Daonan Shen1,Xin Guan3,Yi Ding1()   

  1. 1.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Dept. of Periodontology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2.Dept. of Periodontology, Tianjin Stomatological Hospital, Hospital of Stomatology, Nankai University, Tianjin 300041, China
    3.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & West China School of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2024-03-05 Revised:2024-06-20 Online:2024-11-01 Published:2024-11-04
  • Contact: Yi Ding

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摘要：

目的 探究不同脉冲能量Er: YAG激光照射后钛盘表面形貌与粗糙度的变化，研究不同钛盘表面形貌对细胞生物学行为以及细菌生物学特性的影响，探索Er: YAG激光改性钛表面的合适参数。 方法 将Ti6Al4V钛盘随机分为5组，设置不同的参数（0、50、100、150、200 mJ/P）进行Er: YAG激光照射，通过原子力显微镜观察钛盘表面形貌和粗糙度的变化，使用扫描电子显微镜、酶联免疫吸附试验检测钛盘表面MG63细胞的黏附、增殖和成骨分化能力，通过结晶紫染色和活/死细菌染色观察钛盘表面生物膜的形成。 结果 激光对钛盘表面形貌的改变程度以及钛盘表面粗糙度与脉冲能量成正比，组间差异具有统计学意义（P<0.05）。细胞实验结果显示：100 mJ/P组钛盘表面MG63细胞的早期黏附形态、增殖和成骨分化能力明显优于其他组，差异有统计学意义（P<0.05）。细菌实验结果显示：50 mJ/P组钛盘表面生物膜形成明显低于未处理组，差异有统计学意义（P<0.05）。 结论 在100 mJ/P处理后钛盘表面MG63细胞表现出良好的黏附、增殖及分化能力，在50 mJ/P处理时细菌生物膜形成最少。50~100 mJ/P可能是Er: YAG激光改性种植体表面的适宜参数区间。

关键词: Er: YAG激光, 参数, 钛盘, 表面形貌

Abstract:

Objective To explore the appropriate parameters of the Er: YAG laser modification of titanium (Ti) sur-faces, this work investigated changes in the surface morphologies and surface roughness of Ti discs treated with different pulsed energies of Er: YAG laser, along with the effects of different Ti disc surface morphologies on cell biological be-havior and bacterial biological characteristics. Methods Ti6Al4V discs were randomly divided into five groups for Er: YAG laser irradiation with different parameters (0, 50, 100, 150, and 200 mJ/P). The surface morphologies and roughness changes of the Ti discs were observed by atomic force microscopy. Scanning electron microscopy and enzyme-linked immunosorbent assay were used to detect the adhesion, proliferation, and osteogenic differentiation ability of MG63 cells on the Ti disc surface. Crystal violet staining and live/dead bacteria staining were also employed to observe the biofilm formation on the Ti disc surface. Results Changes in the surface morphologies and surface roughness of the Ti discs were proportional to the pulse energy, and the difference between the groups was statistically significant (P<0.05). The results of cell experiments showed that the early adhesion morphology, proliferation, and osteogenic differentiation ability of the MG63 cell in the 100 mJ/P group were significantly better than those of the other groups (P<0.05). The results of the bacterial experiment also showed that the biofilm formation on the surface of the 50 mJ/P group was significantly lower than that of the untreated group (P<0.05). Conclusion The MG63 cells on the surface of Ti disks showed good adhesion, proliferation, and differentiation ability at 100 mJ/P, as well as showed the least bacterial biofilm formation at 50 mJ/P. Therefore, 50-100 mJ/P may be a suitable parameter range for Er: YAG laser-modified implant surfaces.

Key words: Er: YAG laser, parameters, titanium disc, surface morphology

中图分类号: 

  • R78

图1

不同脉冲能量Er: YAG激光改性后钛盘表面AFM 三维形貌图及粗糙度分析A、B、C、D、E依次为对照组、50 mJ/P组、100 mJ/P组、150 mJ/P、200 mJ/P组三维形貌图 AFM × 15 000；F：钛盘表面粗糙度定量分析。**：P<0.01；****：P<0.000 1。"

图2

不同脉冲能量Er: YAG激光处理后对钛盘表面细胞生物学行为的影响A、B、C、D、E依次为对照组、50 mJ/P、100 mJ/P、150 mJ/P、200 mJ/P组表面细胞黏附图，箭头示细胞 SEM × 5 000；F、G、H、I依次为接种细胞1、3、5、7 d后不同处理组钛盘表面MG63细胞增殖活性CCK-8结果；J：接种细胞7 d后不同处理组钛盘表面细胞成骨分化实验的ALP结果。ns：P>0.05；*：P<0.05；**：P<0.01；***：P<0.001；****：P<0.000 1。"

图3

不同脉冲能量Er: YAG激光处理后对钛盘表面细菌生物学特性的影响A、B、C、D、E依次为对照组、50 mJ/P组、100 mJ/P组、150 mJ/P、200 mJ/P组表面生物膜的活/死细菌荧光染色 共聚焦显微镜 × 60；F：不同脉冲能量Er: YAG激光处理钛盘后表面生物膜0.1%结晶紫染色定量分析。白色箭头示P. gingivalis；黄色箭头示F. nucleatum；蓝色箭头示S. gordonii。****：P<0.000 1。"

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