国际口腔医学杂志

国际口腔医学杂志 ›› 2022, Vol. 49 ›› Issue (2): 132-137.doi: 10.7518/gjkq.2022017

• 论著 • 上一篇    下一篇

口腔诊疗环境细菌群落的时间变化趋势研究

杨加震1,2(),张颖3,刘育含1,李帆4,曾飞1,2,李修珍1,马玉莹1,2,杨芳1()   

  1. 1.青岛大学附属青岛市市立医院口腔医学中心 青岛 266071
    2.大连医科大学口腔医学院 大连 116044
    3.中国人民解放军东部战区总医院口腔科 南京 211299
    4.天津医科大学口腔医院 天津 300070
  • 收稿日期:2021-05-14 修回日期:2021-11-18 出版日期:2022-03-01 发布日期:2022-03-15
  • 通讯作者: 杨芳
  • 作者简介:杨加震,医师,硕士,Email： 17865651571@163.com
  • 基金资助:
    国家自然科学基金(81670979)

Time variation trend of bacterial community in oral diagnosis and treatment environment

Yang Jiazhen1,2(),Zhang Ying3,Liu Yuhan1,Li Fan4,Zeng Fei1,2,Li Xiuzhen1,Ma Yuying1,2,Yang Fang1()   

  1. 1. Stomatology Center, Qingdao Municipal Hospital, Qingdao University, Qingdao 266071, China
    2. School of Stomatology, Dalian Medical University, Dalian 116044, China
    3. Dept. of Stomatology, General Hospital of Eastern Theater of Chinese People’s Liberation Army, Nanjing 211299, China
    4. Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China
  • Received:2021-05-14 Revised:2021-11-18 Online:2022-03-01 Published:2022-03-15
  • Contact: Fang Yang
  • Supported by:
    National Natural Science Foundation of China(81670979)

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摘要：

目的 应用16S rRNA高通量测序技术探究口腔诊疗环境细菌群落特征,及其随着投入使用时间增加的变化趋势,为口腔诊疗环境感染防控提供一定的理论依据。方法 选取新装修口腔内科诊室3间,分别于投入使用前（G1组）、使用1月（G2组）、使用3月（G3组）,采集环境表面样本,利用Illumina MiSeq测序技术对16S rRNA V3-V4区测序,进行细菌群落结构及多样性差异分析。结果 G1组的物种丰富度高于G3组（P<0.05）,3组间的菌落结构的差异具有统计学意义（P<0.05）;细菌群落主要属于变形菌门、厚壁菌门、拟杆菌门等7个门,不同时间组的优势菌门占比相对稳定（P>0.05）;丰度前30的细菌属中检测到埃希氏菌-志贺氏菌、链球菌、假单胞菌等7种潜在致病菌,其中埃希氏菌-志贺氏菌在诊室使用3月时明显增高（P<0.05）,而假单胞菌明显降低（P<0.05）。结论 口腔诊疗环境呈现出丰富的细菌多样性,随着诊室投入使用周期延长,菌落结构更为相似。

关键词: 口腔诊疗环境, 高通量测序, 细菌群落, 交叉感染

Abstract:

Objective This study aimed to explore the characteristics of bacterial community in oral diagnosis and treatment environment by 16S rRNA high-throughput sequencing technology and its changing trend with the increase of application time to provide theoretical basis for the prevention and control of oral diagnosis and treatment environment infection. Methods Three newly renovated dental clinics were selected, and environmental surface samples were collected before use (G1 group), 1 month after use (G2 group) and 3 months after use (G3 group). Then, the 16SrRNA V3-V4 region was sequenced using Illumina MiSeq sequencing technology for the differential analysis of bacterial community structure and diversity. Results The species richness of G1 group was higher than that of G3 group (P<0.05), and a significant difference was observed in the colony structure among the three groups (P<0.05). The bacterial community mainly belonged to seven phyla, including Proteobacteria, Firmicutes and Bacteroidetes, and the proportion of dominant phyla was relatively stable in different time groups (P>0.05). Seven potential pathogenic bacteria, such as Escherichia-Shigella, Streptococcus and Pseudomonas, were detected in the top 30 bacterial genera in abundance, of which Escherichia-Shigella was significantly higher at 3 months of office use (P<0.05), while Pseudomonas was significantly lower (P<0.05). Conclusion The dental diagnosis and treatment environment shows rich bacterial diversity, and the colony structure is more similar with prolonged service cycle in the consultation room.

Key words: oral diagnosis and treatment environment, high-throughput sequencing, bacterial community, cross infection

中图分类号: 

  • R37

图1

样本稀释曲线图"

图2

基于OTU水平的Venn图"

图3

Chao1、Shannon和Simpson指数 *示 P<0.05; ns示 P>0.05。"

图4

3组细菌群落结构比较 A：蓝色表示组内任意2个样本间的未加权Unifrac距离,红色表示不同分组间任意两个样本的未加权Unifrac距离;B：3组样本的分离程度。"

图5

门水平上细菌类群比较"

图6

属水平上细菌类群比较 A：优势菌属的比较;B：致病菌属的比较;*：P<0.05。"

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