Inter J Stomatol ›› 2016, Vol. 43 ›› Issue (3): 273-277.doi: 10.7518/gjkq.2016.03.005

Previous Articles     Next Articles

Differential expression of surface-associated proteins in clinical isolations of Streptococcus mutans

Zhao Xingfu1, Jiang Shan2, Huang Xiaojing2, Yan Fuhua3   

  1. 1. Dept. of Conservative Dentistry and Endodontics II, Tianjin Stomatological Hospital, Stomatological Hospital of Nankai University, Tianjin 300041, China; 2. Dept. of Conservative Dentistry and Endodontics, Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China; 3. Dept. of Senior Expert Diagnosis and Treatment, Hospital of Stomatology, Medical School of Nanjing University, Nanjing 210008, China) This study was supported by the National Natural Science Foundation of China(30500564).
  • Received:2015-06-12 Revised:2015-11-16 Online:2016-05-01 Published:2016-05-01

Abstract: Objective This study aims to investigate the changes in the expression of surface-associated protein at pH7.0 in Streptococcus mutans isolated from clinical samples. Methods The proteins were extracted from the cells at pH7.0 by using the Homer method. The proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDSPAGE) and 2D gel electrophoresis(2DE) followed by image analysis. The proteins were identified by matrix-assisted laser desorption time of flight(MALDI-TOF) mass spectrometry and computer-assisted protein sequence analysis. Results Image analysis revealed that 14 high expression protein loci and 8 specific protein loci exist in Streptococcus mutans 593. Specifically, pyruvate kinase and adenosine triphosphate-binding cassette transporter were modulated, and glucose transferase was highly modulated in Streptococcus mutans 593. Conclusion The difference in protein expression of the two clinical isolations may indicate their distinct cariogenic characters.

Key words: Streptococcus mutans, proteomics, clinical isolation, surface-associated protein, Streptococcus mutans, proteomics, clinical isolation, surface-associated protein

CLC Number: 

  • Q 51

TrendMD: 
[1] 王成龙, 苏东华, 刘佼佼, 等. 不同基因型变异链球菌临床分离株的分离和鉴定[J]. 华西口腔医学杂志, 2013, 31(1):136-140.
Wang CL, Su DH, Liu JJ, et al. Isolation and identification of Streptococcus mutans strains with different genotype from clinical samples[J]. Chin J Stomatol, 2013, 31(1):136-140.
[2] Tahmourespour A, Nabinejad A, Shirian H, et al. Typing of Streptococcus mutans strains isolated from caries free and susceptible subjects by multilocus enzyme electrophoresis[J]. Braz J Microbiol, 2013, 44(3):873-877.
[3] 赵兴福, 黄晓晶, 蔡志宇, 等. 高龋患者与无龋健康人口内变形链球菌蛋白表达差异的初步分析[J].口腔医学研究, 2008, 24(2):127-130.
Zhao XF, Huang XJ, Cai ZY, et al. Proteins differences between Streptococcus mutans strains isolated from caries-active and caries-free individuals by SDS-PAGE[J]. Oral Sci Res, 2008, 24(2):127-130.
[4] Cunningham MW. Pathogenesis of group A streptococcal infections[J]. Clin Microbiol Rev, 2000, 13(3):470-511.
[5] Wilkins JC, Homer KA, Beighton D. Altered protein expression of Streptococcus oralis cultured at low pH revealed by two-dimensional gel electrophoresis [J]. Appl Environ Microbiol, 2001, 67(8):3396-3405.
[6] Wilkins JC, Beighton D, Homer KA. Effect of acidic pH on expression of surface-associated proteins of Streptococcus oralis[J]. Appl Environ Microbiol, 2003, 69(9):5290-5296.
[7] Rabilloud T. Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains[J]. Proteomics, 2002, 2(1):3-10.
[8] 黄晓晶, 刘天佳, 杨锦波, 等. 高龋及无龋者变形链球菌临床分离株致龋性研究Ⅱ:合成细胞外多糖能力的实验研究[J]. 华西口腔医学杂志, 2000, 18(6):419-421.
Huang XJ, Liu TJ, Yang JB, et al. Evaluation of cariogenic potential of Streptococcus mutans isolated from caries-free and-active persons:abilities to synthesize water-soluble and-insoluble glucans[J]. West China J of Stomatol, 2000, 18(6):419-421.
[1] Jing Wang,Yan Wang,Chuandong Wang,Ruijie Huang,Yan Tian,Wei Hu,Jing Zou. Application of liquorice and its extract to the prevention and treatment of oral infections and associated diseases [J]. Inter J Stomatol, 2018, 45(5): 546-552.
[2] Wang Yizhou, Zhang Yaqi, Niu Xuewei, Zhang Zhimin. The groE operon of Streptococcus mutans with its expression and regulation [J]. Inter J Stomatol, 2016, 43(3): 348-351.
[3] Shi Jing, Yan Zhengbin, Hou Jingqiu, Peng Hui. Influence of bracketless invisible aligner technique and conventional technique on the number of Streptococcus mutans and Porphyromonas gingivalis [J]. Inter J Stomatol, 2016, 43(2): 151-154.
[4] Huang Ping, Wan Huchun, He Yonghong.. Differential proteomics of Streptococcus mutans under stress condition [J]. Inter J Stomatol, 2014, 41(1): 92-96.
[5] Ran Jinmei, Zhang Linglin, Li Wei.. Application of bioinformatics in oral medicine [J]. Inter J Stomatol, 2013, 40(4): 516-518.
[6] ZENG Zheng -xiang, ZHOU Xue-dong.. Development of studies on comparative proteomics of Streptococcus mutans [J]. Inter J Stomatol, 2010, 37(4): 477-480.
[7] GUO Shi- liang, JIANG Xin- peng, ZHANG Ying-li.. Techniques in proteomics and application in stomatology [J]. Inter J Stomatol, 2007, 34(05): 361-363.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[7] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[8] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[9] . [J]. Foreign Med Sci: Stomatol, 2004, 31(02): 126 -128 .
[10] . [J]. Inter J Stomatol, 2008, 35(S1): .