Objective This study aimed to investigate the caries status and the association between the caries status and the living habit and healthcare of children living in urban and pasture areas of Tibet. Methods A total of 280 and 376 children were recruited from Lhasa (urban area) and Nagqu (pasture area), respectively, through stratified cluster random sampling. The children’s caries status and their living habits and healthcare behavior were collected using questionnaires. Data were analyzed by SPSS 21.0 software. Results In Lhasa, 124 children (59 males and 65 females) of primary dentition and 156 children (81 males and 75 females) of mixed dentition were recorded. The overall mean decay-missedfilled-teeth (dmft) was 4.54. The mean dmft of children of primary dentition was 4.59, and the dmft values of males and females were 4.41 and 4.86, respectively. The overall mean dmft was 3.55. The mean dmft of children of mixed dentition was 4.51, and the dmft values of males and females were 4.16 and 4.88, respectively. In Nagqu, 190 children (109 males and 81 females) of primary dentition and 186 children (97 males and 89 females) of mixed dentition were recorded. The overall mean dmft was 3.55. The mean dmft of children of primary dentition was 3.31, and the dmft values of males and females were 3.83 and 2.61, respectively. The mean dmft of children of mixed dentition was 3.79, and the dmft values of males and females were 3.68 and 3.91, respectively. The overall dmft and primary dentition dmft of children in Lhasa were higher than those of children in Nagqu (P<0.05). Primary molars were the teeth with the highest caries rate, whereas mandibular incisors were the teeth with the lowest caries rate in both sampling areas. Gender had no significant contribution to the caries rate. Children who frequently visited doctors for non oral diseases had higher rates of caries in Lhasa and Nagqu, respectively (P<0.01). About 81.4% of children from Lhasa brushed their teeth at least once a day, but only 8.9% of the children used fluoride toothpaste. In addition, 56.1% and 36.4% of the children’s families had never consulted with a dentist. In Nagqu, the results were 59.6%, 18.6%, 73.4%, and 51.3%, respectively. Conclusion The children’s caries status in Tibet is still severe. Further studies should be conducted to strengthen oral healthcare education to control caries among children in Tibet.