Int J Stomatol

Int J Stomatol ›› 2025, Vol. 52 ›› Issue (2): 195-204.doi: 10.7518/gjkq.2025008

• Original Articles • Previous Articles     Next Articles

Mechanism of transferrin-modified liposomal curcumin on proliferation inhibition of oral squamous cell carcinoma

Xueqin Wei(),Kai Ba   

  1. Dept. of Stomatology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2024-03-09 Revised:2024-07-24 Online:2025-03-01 Published:2025-03-01
  • Contact: Xueqin Wei E-mail:dentistwendy@qq.com
  • Supported by:
    Soft Science Project and Joint Construction Project of Henan Medical Science and Technology Research Plan(LHGJ20220361);Henan Province Science and Technology Research Project(232102310491)

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Abstract:

Objective This study aimed to investigate the effect of transferrin (Tf)-modified curcumin (Cur)-loaded liposomes (Lips) on the oral squamous cell carcinoma cell line HN4. Methods Cur-Lips and Tf-Cur-Lips were prepared, and their regulatory effects on the internal and external disposal of curcumin were investigated during Cur release in vitro and pharmacokinetics in vivo in rats. Then, HN4 cells were treated with Cur, Cur-Lips, and Tf-Cur-Lips at different concentrations successively, and cell counting kit-8 was used to detect the effects of different experimental groups on HN4 cell proliferation. Finally, the expression levels of apoptosis-related genes P53 and Fas and the molecular mechanism of transferrin modification on Cur-Lips inhibiting the proliferation and apoptosis of HN4 cells were investigated through real-time fluorescence quantitative polymerase chain reaction. Results Compared with Cur, Cur-Lips considerably prolonged metabolic time, and transferrin modification further improved the stability of Cur and prolonged the metabolic time of Cur-Lips. Compared with Cur and Cur-Lips, Tf-Cur-Lips markedly enhanced the inhibitory effect on the HN4 cell proliferation and up-regulated the expression of P53 and Fas. Conclusion Tf-Cur-Lips have a stronger inhibitory effect on oral squamous cell carcinoma cell HN4 than Cur and Cur-Lips.

Key words: curcumin, liposomes, transferrin, HN4 cells, cell apoptosis

CLC Number: 

  • R780.1

TrendMD: 

Tab 1

Primer sequences of target genes"

基因引物序列（5’-3’）
GAPDH上游：GACGGCCGCATCTTCTTGTGC
下游：TGCAAATGGCAGCCCTGGTGA
P53上游：CTTTGAGGTGCGTGTTT
下游：CAGTGCTCGCTTAGTGC
Fas上游：GTGATGAAGGGCATGGTTTAG
下游：GCATTTGGTGTTGCTGGTT

Tab 2

Particle size and surface potential of Cur-Lips and Tf-Cur-Lips"

组别粒径/nm分散系数表面电位/mV
Cur-Lips183.0±2.80.19±0.03-28.8±0.3
Tf-Cur-Lips194.0±2.1*0.20±0.05-29.3±0.5

Fig 1

Particle size and potential profile"

Fig 2

Scanning electron microscope photographs"

Fig 3

Cumulative release rate of Cur in vitro"

Fig 4

The inhibitory effect of Cur, Cur-Lips and Tf-Cur-Lips on HN4 cells proliferation at four concentrations"

Fig 5

Effect of Cur, Cur-Lips and Tf-Cur-Lips on the expression of P53 and Fas gene of HN4"

Fig 6

Pharmacokinetics profiles of rats"

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