Int J Stomatol

Int J Stomatol ›› 2024, Vol. 51 ›› Issue (6): 713-721.doi: 10.7518/gjkq.2024083

• Original Articles • Previous Articles    

Effect of Er: YAG laser with different pulse energies on the surface morphologies and biological properties of titanium discs

Yi Liu1(),Jiangshan Qiu2,Daonan Shen1,Xin Guan3,Yi Ding1()   

  1. 1.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Dept. of Periodontology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2.Dept. of Periodontology, Tianjin Stomatological Hospital, Hospital of Stomatology, Nankai University, Tianjin 300041, China
    3.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & West China School of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2024-03-05 Revised:2024-06-20 Online:2024-11-01 Published:2024-11-04
  • Contact: Yi Ding E-mail:liuyi66116161@163.com;yiding2000@126.com

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Abstract:

Objective To explore the appropriate parameters of the Er: YAG laser modification of titanium (Ti) sur-faces, this work investigated changes in the surface morphologies and surface roughness of Ti discs treated with different pulsed energies of Er: YAG laser, along with the effects of different Ti disc surface morphologies on cell biological be-havior and bacterial biological characteristics. Methods Ti6Al4V discs were randomly divided into five groups for Er: YAG laser irradiation with different parameters (0, 50, 100, 150, and 200 mJ/P). The surface morphologies and roughness changes of the Ti discs were observed by atomic force microscopy. Scanning electron microscopy and enzyme-linked immunosorbent assay were used to detect the adhesion, proliferation, and osteogenic differentiation ability of MG63 cells on the Ti disc surface. Crystal violet staining and live/dead bacteria staining were also employed to observe the biofilm formation on the Ti disc surface. Results Changes in the surface morphologies and surface roughness of the Ti discs were proportional to the pulse energy, and the difference between the groups was statistically significant (P<0.05). The results of cell experiments showed that the early adhesion morphology, proliferation, and osteogenic differentiation ability of the MG63 cell in the 100 mJ/P group were significantly better than those of the other groups (P<0.05). The results of the bacterial experiment also showed that the biofilm formation on the surface of the 50 mJ/P group was significantly lower than that of the untreated group (P<0.05). Conclusion The MG63 cells on the surface of Ti disks showed good adhesion, proliferation, and differentiation ability at 100 mJ/P, as well as showed the least bacterial biofilm formation at 50 mJ/P. Therefore, 50-100 mJ/P may be a suitable parameter range for Er: YAG laser-modified implant surfaces.

Key words: Er: YAG laser, parameters, titanium disc, surface morphology

CLC Number: 

  • R78

TrendMD: 

Fig 1

Analysis of three-dimensional morphology and surface roughness of titanium discs modified by Er: YAG laser with different pulse energy by AFM"

Fig 2

The effect of Er: YAG laser treatment with different pulse energy on the cell biological behavior of titanium discs"

Fig 3

Effect of Er: YAG laser treatment at different pulse energies on the bacterial biological properties of titanium discs"

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