Int J Stomatol

Inter J Stomatol ›› 2014, Vol. 41 ›› Issue (1): 23-25.doi: 10.7518/gjkq.2014.01.006

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Isolation and identification of exosomes secreted from cell line Tca8113 in tongue cancer

Li Jin1, Liao Guiqing2, Chen Jufeng1, Liu Haichao2, Su Yuxiong2, Feng Lianqiang3, Luo Xiaofeng4.   

  1. 1. Dept. of Stomatology, The First Hospital of Foshan, Foshan 528000, China; 2. Dept. of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou 510055, China; 3. Dept. of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; 4. Dept. of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
  • Received:2013-06-28 Revised:2013-10-21 Online:2014-01-01 Published:2014-01-01

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Abstract:

Objective This study aims to verify whether Tca8113 can secrete exosomes, and to identify a new method for oral tumor immunotherapy. Methods Exosomes were isolated and purified from the culture supernatant of Tca8113 by ultraspeed centrifugation and density gradient centrifugation, and were subsequently confirmed using transmission electron microscopy and Western blot. Results Exosomes were successfully isolated and purified from the culture supernatant of Tca8113. Tca8113 contained protein and had a structure similar to other cells, as reported in previous studies. Conclusion Tca8113 can secrete exosomes, and contains numerous types of proteins that are associated with tumor immunity.

Key words: exosomes, western blot, transmission electron microscopy, tumor immunity


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[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[7] . [J]. Foreign Med Sci: Stomatol, 2005, 32(06): 458 -460 .
[8] . [J]. Foreign Med Sci: Stomatol, 2005, 32(06): 452 -454 .
[9] . [J]. Inter J Stomatol, 2008, 35(S1): .
[10] . [J]. Inter J Stomatol, 2008, 35(S1): .