关键词: 微振动, 人脐静脉血管内皮细胞, 增殖

Abstract:

Objective To evaluate the proliferation and differentiation of human umbilical vein endothelial cell（HUVEC） under microvibration. Methods HUVECs were randomly divided into three groups: Group A was blank control group, group B was vibrated under 80 Hz, 30 min per day, group C was vibrated under 40 Hz, 30 min per day; observed HUVEC proliferations based on micro-vibration duration on 0, 3, 5, 7 d. Methyl thiazolyl tetrazolium（MTT） assay was used to detect cell proliferation and growth curve. According to the result of the growth curve, we took another HUVECs divided into 4 groups：Group a was vibrated immediately, group b was blank control of group a, group c was vibrated during three days, group d was blank control of group c. Western blot assay was used to detect secretion of intercellular adhesion molecule（ICAM）-1, endothelial nitric oxide synthase（eNOS） and phosphorylate-eNOS. Results MTT growth curve showed the proliferation mainly from the first to the third day, stepped into the plateau period from the fifth day. Group C proliferated more quickly than in group B from the first day, more quickly than group A from the third day, proliferation capacity of group B was always lower than group A, and the differences were statistically significant（P<0.05）. The expressions of ICAM-1 and PeNOS in group c were the highest in all groups. Conclusion The low amplitude high frequency microvibration promoted ICAM-1 and P-eNOS expression, enhanced cell adhesion.

Key words: microvibration, human umbilical vein endothelial cell, proliferation