Int J Stomatol

Inter J Stomatol ›› 2007, Vol. 34 ›› Issue (05): 322-324.

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Effect of hyper thermia on the expr ession of nm23- H1 gene in tongue squamous car cinoma cells

FEI Jimin1, LIU Liu2, LI Mei1，YAO Qian1, CHEN Yun1, XI Yan1.   

  1. 1. Dept. of Head and Neck Surgery, The Third Affiliated Hospital of Kunming Medical College, Kunming 650118, China; 2. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, China
  • Online:2007-09-20 Published:2007-09-20

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Abstract:

Objective To investigate the mechanism of hyperthermia treatment of tongue cancer by observing the relation of expression of nm23- H1 gene and the infiltration and metastasis of the cancer. Methods Tongue squamous carcinoma cells（Tca8113） heated in vitro was observed and analysed through transmission electron microscope（ TEM）, immunohistochemistry, flow cytometry, then determined the quantities of nm23- H1 and ultrastructure. Results The organelles increased after heated by hyperthermia. In the heated cells, the expression quantities of nm23- H1 increased, but this effect couldn′t be changed as the cultured time prolonged. Conclusion Hyperthermia can suppress cancer cell invasion and metastasis by adjusting the expression of nm23- H1. It is the optimal time for hyperthermia treatment to heat 30 minutes at 43 ℃.

Key words: human tongue carcinoma, Tca8113 cell lines, hyperthermia, gene nm23- H1


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