Int J Stomatol

Int J Stomatol ›› 2022, Vol. 49 ›› Issue (2): 132-137.doi: 10.7518/gjkq.2022017

• Original Articles • Previous Articles     Next Articles

Time variation trend of bacterial community in oral diagnosis and treatment environment

Yang Jiazhen1,2(),Zhang Ying3,Liu Yuhan1,Li Fan4,Zeng Fei1,2,Li Xiuzhen1,Ma Yuying1,2,Yang Fang1()   

  1. 1. Stomatology Center, Qingdao Municipal Hospital, Qingdao University, Qingdao 266071, China
    2. School of Stomatology, Dalian Medical University, Dalian 116044, China
    3. Dept. of Stomatology, General Hospital of Eastern Theater of Chinese People’s Liberation Army, Nanjing 211299, China
    4. Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China
  • Received:2021-05-14 Revised:2021-11-18 Online:2022-03-01 Published:2022-03-15
  • Contact: Fang Yang E-mail:17865651571@163.com;yangf82@sina.com
  • Supported by:
    National Natural Science Foundation of China(81670979)

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Abstract:

Objective This study aimed to explore the characteristics of bacterial community in oral diagnosis and treatment environment by 16S rRNA high-throughput sequencing technology and its changing trend with the increase of application time to provide theoretical basis for the prevention and control of oral diagnosis and treatment environment infection. Methods Three newly renovated dental clinics were selected, and environmental surface samples were collected before use (G1 group), 1 month after use (G2 group) and 3 months after use (G3 group). Then, the 16SrRNA V3-V4 region was sequenced using Illumina MiSeq sequencing technology for the differential analysis of bacterial community structure and diversity. Results The species richness of G1 group was higher than that of G3 group (P<0.05), and a significant difference was observed in the colony structure among the three groups (P<0.05). The bacterial community mainly belonged to seven phyla, including Proteobacteria, Firmicutes and Bacteroidetes, and the proportion of dominant phyla was relatively stable in different time groups (P>0.05). Seven potential pathogenic bacteria, such as Escherichia-Shigella, Streptococcus and Pseudomonas, were detected in the top 30 bacterial genera in abundance, of which Escherichia-Shigella was significantly higher at 3 months of office use (P<0.05), while Pseudomonas was significantly lower (P<0.05). Conclusion The dental diagnosis and treatment environment shows rich bacterial diversity, and the colony structure is more similar with prolonged service cycle in the consultation room.

Key words: oral diagnosis and treatment environment, high-throughput sequencing, bacterial community, cross infection

CLC Number: 

  • R37

TrendMD: 

Fig 1

Rarefaction curves of three groups"

Fig 2

Venn diagram based on OTU level"

Fig 3

Index of Chao1, Shannon and Simpson"

Fig 4

Comparison bacterial community structure in three groups"

Fig 5

Comparison of bacterial groups at phylum level"

Fig 6

Comparison of bacterial groups at genus level"

[1] Mark Welch JL, Ramírez-Puebla ST, Borisy GG. Oral microbiome geography: micron-scale habitat and niche[J]. Cell Host Microbe, 2020, 28(2): 160-168.
doi: 10.1016/j.chom.2020.07.009
[2] Rautemaa R, Nordberg A, Wuolijoki-Saaristo K, et al. Bacterial aerosols in dental practice-a potential hospital infection problem[J]. J Hosp Infect, 2006, 64(1): 76-81.
pmid: 16820249
[3] Vidana R, Sillerström E, Ahlquist M, et al. Potential for nosocomial transmission of Enterococcus faecalis from surfaces in dental operatories[J]. Int Endod J, 2015, 48(6): 518-527.
doi: 10.1111/iej.12342 pmid: 25066305
[4] Ricci ML, Fontana S, Pinci F, et al. Pneumonia associated with a dental unit waterline[J]. Lancet, 2012, 379(9816): 684.
doi: 10.1016/S0140-6736(12)60074-9
[5] Boyce JM. Modern technologies for improving cleaning and disinfection of environmental surfaces in hospitals[J]. Antimicrob Resist Infect Control, 2016, 5: 10.
doi: 10.1186/s13756-016-0111-x
[6] Stewart EJ. Growing unculturable bacteria[J]. J Bacteriol, 2012, 194(16): 4151-4160.
doi: 10.1128/JB.00345-12 pmid: 22661685
[7] Merikanto I, Laakso JT, Kaitala V. Invasion ability and disease dynamics of environmentally growing opportunistic pathogens under outside-host competition[J]. PLoS One, 2014, 9(11): e113436.
doi: 10.1371/journal.pone.0113436
[8] Zhang Y, Ping YF, Zhou RY, et al. High throughput sequencing-based analysis of microbial diversity in dental unit waterlines supports the importance of providing safe water for clinical use[J]. J Infect Public Health, 2018, 11(3): 357-363.
doi: 10.1016/j.jiph.2017.09.017
[9] Costa D, Mercier A, Gravouil K, et al. Pyrosequencing analysis of bacterial diversity in dental unit waterlines[J]. Water Res, 2015, 81: 223-231.
doi: 10.1016/j.watres.2015.05.065
[10] Rognes T, Flouri T, Nichols B, et al. VSEARCH: a versatile open source tool for metagenomics[J]. PeerJ, 2016, 4: e2584.
doi: 10.7717/peerj.2584
[11] Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nat Methods, 2010, 7(5): 335-336.
doi: 10.1038/nmeth.f.303 pmid: 20383131
[12] Shobo CO, Alisoltani A, Abia ALK, et al. Bacterial diversity and functional profile of microbial populations on surfaces in public hospital environments in South Africa: a high throughput metagenomic analysis[J]. Sci Total Environ, 2020, 719: 137360.
doi: 10.1016/j.scitotenv.2020.137360
[13] Chopyk J, Akrami K, Bavly T, et al. Temporal variations in bacterial community diversity and composition throughout intensive care unit renovations[J]. Microbiome, 2020, 8(1): 86.
doi: 10.1186/s40168-020-00852-7
[14] Rampelotto PH, Sereia AFR, de Oliveira LFV, et al. Exploring the hospital microbiome by high-resolution 16S rRNA profiling[J]. Int J Mol Sci, 2019, 20(12): E3099.
[15] Verma D, Garg PK, Dubey AK. Insights into the human oral microbiome[J]. Arch Microbiol, 2018, 200(4): 525-540.
doi: 10.1007/s00203-018-1505-3
[16] Yu XL, Chan Y, Zhuang LF, et al. Intra-oral single-site comparisons of periodontal and peri-implant microbiota in health and disease[J]. Clin Oral Implants Res, 2019, 30(8): 760-776.
doi: 10.1111/clr.v30.8
[17] Liu G, Wu C, Abrams WR, et al. Structural and functional characteristics of the microbiome in deep-dentin caries[J]. J Dent Res, 2020, 99(6): 713-720.
doi: 10.1177/0022034520913248 pmid: 32196394
[18] Wang XW, Zhao ZB, Tang N, et al. Microbial community analysis of saliva and biopsies in patients with oral lichen planus[J]. Front Microbiol, 2020, 11: 629.
doi: 10.3389/fmicb.2020.00629
[19] Xu J, Chen N, Wu Z, et al. 5-aminosalicylic acid alters the gut bacterial microbiota in patients with ulcerative colitis[J]. Front Microbiol, 2018, 9: 1274.
doi: 10.3389/fmicb.2018.01274
[20] Fujitani S, Sun HY, Yu VL, et al. Pneumonia due to Pseudomonas aeruginosa: part Ⅰ: epidemiology, clinical diagnosis, and source[J]. Chest, 2011, 139(4): 909-919.
doi: S0012-3692(11)60194-3 pmid: 21467058
[21] Mittal R, Aggarwal S, Sharma S, et al. Urinary tract infections caused by Pseudomonas aeruginosa: a minireview[J]. J Infect Public Health, 2009, 2(3): 101-111.
doi: 10.1016/j.jiph.2009.08.003
[22] Wu DC, Chan WW, Metelitsa AI, et al. Pseudomonas skin infection: clinical features, epidemiology, and management[J]. Am J Clin Dermatol, 2011, 12(3): 157-169.
doi: 10.2165/11539770-000000000-00000
[23] Willcox MD. Pseudomonas aeruginosa infection and inflammation during contact lens wear: a review[J]. Optom Vis Sci, 2007, 84(4): 273-278.
pmid: 17435510
[24] Ribeiro LF, Lopes EM, Kishi LT, et al. Microbial community profiling in intensive care units expose limitations in current sanitary standards[J]. Front Public Health, 2019, 7: 240.
doi: 10.3389/fpubh.2019.00240
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