Int J Stomatol

Int J Stomatol ›› 2022, Vol. 49 ›› Issue (2): 132-137.doi: 10.7518/gjkq.2022017

• Original Articles • Previous Articles     Next Articles

Time variation trend of bacterial community in oral diagnosis and treatment environment

Yang Jiazhen1,2(),Zhang Ying3,Liu Yuhan1,Li Fan4,Zeng Fei1,2,Li Xiuzhen1,Ma Yuying1,2,Yang Fang1()   

  1. 1. Stomatology Center, Qingdao Municipal Hospital, Qingdao University, Qingdao 266071, China
    2. School of Stomatology, Dalian Medical University, Dalian 116044, China
    3. Dept. of Stomatology, General Hospital of Eastern Theater of Chinese People’s Liberation Army, Nanjing 211299, China
    4. Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China
  • Received:2021-05-14 Revised:2021-11-18 Online:2022-03-01 Published:2022-03-15
  • Contact: Fang Yang E-mail:17865651571@163.com;yangf82@sina.com
  • Supported by:
    National Natural Science Foundation of China(81670979)

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Abstract:

Objective This study aimed to explore the characteristics of bacterial community in oral diagnosis and treatment environment by 16S rRNA high-throughput sequencing technology and its changing trend with the increase of application time to provide theoretical basis for the prevention and control of oral diagnosis and treatment environment infection. Methods Three newly renovated dental clinics were selected, and environmental surface samples were collected before use (G1 group), 1 month after use (G2 group) and 3 months after use (G3 group). Then, the 16SrRNA V3-V4 region was sequenced using Illumina MiSeq sequencing technology for the differential analysis of bacterial community structure and diversity. Results The species richness of G1 group was higher than that of G3 group (P<0.05), and a significant difference was observed in the colony structure among the three groups (P<0.05). The bacterial community mainly belonged to seven phyla, including Proteobacteria, Firmicutes and Bacteroidetes, and the proportion of dominant phyla was relatively stable in different time groups (P>0.05). Seven potential pathogenic bacteria, such as Escherichia-Shigella, Streptococcus and Pseudomonas, were detected in the top 30 bacterial genera in abundance, of which Escherichia-Shigella was significantly higher at 3 months of office use (P<0.05), while Pseudomonas was significantly lower (P<0.05). Conclusion The dental diagnosis and treatment environment shows rich bacterial diversity, and the colony structure is more similar with prolonged service cycle in the consultation room.

Key words: oral diagnosis and treatment environment, high-throughput sequencing, bacterial community, cross infection

CLC Number: 

  • R37

TrendMD: 

Fig 1

Rarefaction curves of three groups"

Fig 2

Venn diagram based on OTU level"

Fig 3

Index of Chao1, Shannon and Simpson"

Fig 4

Comparison bacterial community structure in three groups"

Fig 5

Comparison of bacterial groups at phylum level"

Fig 6

Comparison of bacterial groups at genus level"

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