国际口腔医学杂志

国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (1): 26-31.doi: 10.7518/gjkq.2018.01.005

• 论著 • 上一篇    下一篇

溶血磷脂酸对牙髓细胞中β-连环蛋白核转位的影响及机制初探

房宏志1, 杨惠2, 邵美瑛3, 胡涛4   

  1. 1.成都市第三人民医院口腔科 成都 610031
    2.口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院全科门诊 成都 610041
    3.四川大学华西第四医院口腔科 成都 610041
    4.口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院预防科 成都 610041
  • 收稿日期:2017-05-15 修回日期:2017-10-19 出版日期:2018-01-01 发布日期:2018-01-01
  • 通讯作者: 邵美瑛,讲师,博士,Email：shaomeiying2013@163.com
  • 作者简介:房宏志,副主任医师,硕士,Email：1127958575@qq.com
  • 基金资助:

    国家自然科学基金（81400504）; 四川省科学技术厅应用基础研究项目（2014JY0073,2013JY0164）; 中国博士后科学基金面上项目（2014M562332）

The effects and underlying mechanism of lysophosphatidic acid in β-catenin nuclear translocation of dental pulp cells

Fang Hongzhi1, Yang Hui2, Shao Meiying3, Hu Tao4   

  1. 1. Dept. of Stomatology, The Third People’s Hospital of Chengdu, Chengdu 610031, China
    2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of General Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    3. Dept. of Stomatology, No. 4 West China Teaching Hospital, Sichuan University, Chengdu 610041, China
    4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2017-05-15 Revised:2017-10-19 Online:2018-01-01 Published:2018-01-01
  • Supported by:

    This study was supported by National Natural Science Foundation of China (81400504), Applied Basic Research Project of Sichuan Provincial Science and Technology Department (2014JY0073, 2013JY0164) and China Postdoctoral Science Foundation (2014M562332).

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摘要：

目的 采用体外培养的牙髓细胞,探讨溶血磷脂酸（LPA）对牙髓细胞内β-连环蛋白释放、活化及核转位的影响。方法 采用LPA刺激牙髓细胞,Y-27632阻断剂抑制Rho相关蛋白激酶（ROCK）,通过免疫荧光和Western blot检测Rho/ROCK信号通路对牙髓细胞β-连环蛋白释放、活化及核转位的影响。结果 LPA刺激牙髓细胞3 h,β-连环蛋白向核膜边缘集聚;刺激6、10 h,见部分牙髓细胞的β-连环蛋白转运至细胞核。用Y-27632预先阻断ROCK后,能够抑制LPA所引起的β-连环蛋白核内转位。Western blot检测结果显示,LPA促进β-连环蛋白表达及活化,Y-27632能够在一定程度上抑制LPA介导的β-连环蛋白的活化水平。结论 LPA能够通过Rho/ROCK通路影响牙髓细胞β-连环蛋白的释放、活化及核转位。

关键词: 牙髓细胞, 溶血磷脂酸, Rho相关蛋白激酶, β-连环蛋白

Abstract:

Objective The aim of our study is to investigate the biological effects of lysophosphatidic acid (LPA) on β-catenin accumulation, activation, and nuclear translocation of cultured human dental pulp cells (DPCs) ex vivo. Methods LPA was used to stimulate dental pulp cells, and Y-27632 was used to inhibit Rho-associated protein kinase (ROCK). Fluorescence microscopy and Western blot were used to detect the effects of Rho/ROCK on β-catenin accumulation, activation, and nuclear transloca- tion. Results β-catenin accumulated around the nuclear membrane after LPA stimulation of DPC for 3 h. When treated for 6 or 10 h with LPA, β-catenin translocated into the nucleus in some dental pulp cells; however, nuclear translocation of β-catenin was abrogated by Y-27632. Results of Western blot showed that LPA promoted β-catenin expression and activation. ROCK inhibition also degraded the level of β-catenin activation. Conclusion LPA regulated β-catenin accumulation, activation, and nuclear translocation via the Rho/ROCK signaling pathway.

Key words: dental pulp cell, lysophosphatidic acid, Rho-associated protein kinase, β-catenin

中图分类号: 

  • R37
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