国际口腔医学杂志 ›› 2016, Vol. 43 ›› Issue (2): 228-232.doi: 10.7518/gjkq.2016.02.025
邱伟,周学东,李明云
Qiu Wei, Zhou Xuedong, Li Mingyun
摘要: 丙氨酸消旋酶(ALR)是以吡哆醛磷酸为辅酶,催化L-丙氨酸与D-丙氨酸相互转化的一类酶,广泛分布于低等生物,与由细菌引起的疾病密切相关。近年来ALR已成为设计抗菌药物的又一理想靶位。变异链球菌是口腔龋病主要致病菌,产酸耐酸、黏附能力和合成细胞内外多糖能力是其主要的致龋毒力。本文对ALR的分类、结构特征、生理功能及实际应用等方面进行系统阐述,为进一步研究ALR与变异链球菌致病毒力的关系,发展龋病抗菌药物候选靶标提供理论基础。
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[1] Neuhaus FC, Baddiley J. A continuum of anionic charge: structures and functions of D-alanyl-teichoic acids in gram-positive bacteria[J]. Microbiol Mol Biol Rev, 2003, 67(4):686-723. [2] Milligan DL, Tran SL, Strych U, et al. The alanine racemase of Mycobacterium smegmatis is essential for growth in the absence of D-alanine[J]. J Bacteriol, 2007, 189(22):8381-8386. [3] Chesnokova ON, McPherson SA, Steichen CT, et al. The spore-specific alanine racemase of Bacillus anthracis and its role in suppressing germination during spore development[J]. J Bacteriol, 2009, 191(4):1303-1310. [4] Hoffmann K, Schneider-Scherzer E, Kleinkauf H, et al. Purification and characterization of eucaryotic alanine racemase acting as key enzyme in cyclosporin biosynthesis[J]. J Biol Chem, 1994, 269(17):12710-12714. [5] Cheng YQ, Walton JD. A eukaryotic alanine racemase gene involved in cyclic peptide biosynthesis[J]. J Biol Chem, 2000, 275(7):4906-4911. [6] Uo T, Yoshimura T, Tanaka N, et al. Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase[J]. J Bacteriol, 2001, 183(7):2226-2233. [7] Matsushima O, Hayashi YS. Metabolism of D-and L-alanine and regulation of intracellular free amino acid levels during salinity stress in a brackish-water bivalve Corbicula japonica[J]. Comp Biochem Physiol, 1992, 102(92):465-471. [8] Nomura T, Yamamoto I, Morishita F, et al. Purification and some properties of alanine racemase from a bivalve mollusc Corbicula japonica[J]. J Exp Zool, 2001, 289(1):1-9. [9] Shibata K, Shirasuna K, Motegi K, et al. Purification and properties of alanine racemase from crayfish Procambarus clarkii[J]. Comp Biochem Physiol B Biochem Mol Biol, 2000, 126(4):599-608. [10] Fujita E, Okuma E, Abe H. Occurrence of alanine racemase in crustaceans and the changes of the properties during seawater acclimation of crayfish[J]. Comp Biochem Physiol, 1997, 116(96):83-87. [11] Yoshikawa N, Dhomae N, Takio K, et al. Purification, properties, and partial amino acid sequences of alanine racemase from the muscle of the black tiger prawn Penaeus monodon[J]. Comp Biochem Physiol B Biochem Mol Biol, 2002, 133(3):445-453. [12] Ono K, Yanagida K, Oikawa T, et al. Alanine racemase of alfalfa seedlings(Medicago sativa L.): first evidence for the presence of an amino acid racemase in plants[J]. Phytochemistry, 2006, 67(9):856-860. [13] Watanabe A, Yoshimura T, Mikami B, et al. Reaction mechanism of alanine racemase from Bacillus stearothermophilus: X-ray crystallographic studies of the enzyme bound with N-(5’-phosphopyridoxyl) alanine[J]. J Biol Chem, 2002, 277(21):19166-19172. [14] Kang L, Shaw AC, Xu D, et al. Upregulation of Met C is essential for D-alanine-independent growth of an alr/dadX-deficient Escherichia coli strain[J]. J Bacteriol, 2011, 193(5):1098-1106. [15] Ju J, Yokoigawa K, Misono H, et al. Cloning of alanine racemase genes from Pseudomonas fluorescens strains and oligomerization states of gene products expressed in Escherichia coli[J]. J Biosci Bioeng, 2005, 100(4):409-417. [16] Radkov AD, Moe LA. Amino acid racemization in Pseudomonas putida KT2440[J]. J Bacteriol, 2013, 195(22):5016-5024. [17] 张彩凤, 徐书景, 李艳红, 等. 丙氨酸消旋酶的研究进展[J]. 安徽农业科学, 2011, 39(15):8836-8839. Zhang CF, Xu SJ, Li YH, et al. Research progress of alanine racemase[J]. J Anhui Agri Sci, 2011, 39(15):8836-8839. [18] Scaletti ER, Luckner SR, Krause KL. Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus(Mu50)[J]. Acta Crystallogr D Biol Crystallogr, 2012, 68(Pt 1):82-92. [19] Priyadarshi A, Lee EH, Sung MW, et al. Structural insights into the alanine racemase from Enterococcus faecalis[J]. Biochim Biophys Acta, 2009, 1794(7):1030-1040. [20] Chacon O, Bermudez LE, Zinniel DK, et al. Impairment of D-alanine biosynthesis in Mycobacterium smegmatis determines decreased intracellular survival in human macrophages[J]. Microbiology, 2009, 155 (Pt 5):1440-1450. [21] Venir E, Del Torre M, Cunsolo V, et al. Involvement of alanine racemase in germination of Bacillus cereus spores lacking an intact exosporium[J]. Arch Microbiol, 2014, 196(2):79-85. [22] Kolodkin-Gal I, Romero D, Cao S, et al. D-amino acids trigger biofilm disassembly[J]. Science, 2010, 328(5978):627-629. [23] Hochbaum AI, Kolodkin-Gal I, Foulston L, et al. Inhibitory effects of D-amino acids on Staphylococcus aureus biofilm development[J]. J Bacteriol, 2011, 193(20):5616-5622. [24] Halouska S, Fenton RJ, Zinniel DK, et al. Metabolomics analysis identifies D-alanine-D-alanine ligase as the primary lethal target of D-cycloserine in mycobacteria[J]. J Proteome Res, 2014, 13(2):1065-1076. [25] Tripathi RP, Tripathi R, Tiwari VK, et al. Synthesis of glycosylated beta-amino acids as new class of antitubercular agents[J]. Eur J Med Chem, 2002, 37(9):773-781. [26] Dong LI, Zhou R. Progress in the research of antituberculous drugs(?)[J]. Chin J New Drugs, 2009, 18(1):35-42. [27] Ciustea M, Mootien S, Rosato AE, et al. Thiadiazolidinones: a new class of alanine racemase inhibitors with antimicrobial activity against methicillin-resistant Staphylococcus aureus[J]. Biochem Pharmacol, 2012, 83(3):368-377. [28] Nguyen TT, Mathiesen G, Fredriksen L, et al. A food-grade system for inducible gene expression in Lactobacillus plantarum using an alanine racemaseencoding selection marker[J]. J Agric Food Chem, 2011, 59(10):5617-5624. [29] 刘开云, 孙红武, 张卫军, 等. 变异链球菌防治药物的研究现状[J]. 国际口腔医学杂志, 2012, 39(5):683-685. Liu KY, Sun HW, Zhang WJ, et al. Research progress on drug prevention of Streptococcus mutans [J]. Int J Stomatol, 2012, 39(5):683-685. [30] 陆慧, 李永亮, 孙育杰, 等. 变异链球菌alr缺失突变株的构建及初步观察[J]. 微生物学杂志, 2013, 33(4):9-13. Lu H, Li YL, Sun YJ, et al. Construction and preliminary observation of Streptococcus mutans alr deletion mutant[J]. J Microbiol, 2013, 33(4):9-13. (本文采编 王晴) |
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