国际口腔医学杂志

国际口腔医学杂志 ›› 2025, Vol. 52 ›› Issue (1): 61-68.doi: 10.7518/gjkq.2025011

• 论著 • 上一篇    下一篇

大麻素Ⅰ型受体抑制与激活对大鼠硬腭黏膜创伤愈合的影响

龙海轮1,2(),苏旭2,吾凡别克·巴合提2,张勇3,陈晓涛1,2()   

  1. 1.石河子大学医学院口腔医学系 石河子 832003
    2.新疆维吾尔自治区人民医院口腔科 乌鲁木齐 830001
    3.北京大学口腔医学院 北京 100081
  • 收稿日期:2024-01-22 修回日期:2024-05-23 出版日期:2025-01-01 发布日期:2025-01-11
  • 通讯作者: 陈晓涛
  • 作者简介:龙海轮，硕士，Email：<email>kezekq10@163.com</email>
  • 基金资助:
    国家自然科学基金(82260195)

Effects of cannabinoid type Ⅰ receptor inhibition and activation on hard-palate mucosal wound healing in rats

Hailun Long1,2(),Xu Su2,Wufanbieke Baheti2,Yong Zhang3,Xiaotao Chen1,2()   

  1. 1.Dept. of Stomatology, School of Medicine, Shihezi University, Shihezi 832003, China
    2.Dept. of Stomatology, People ’ s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
    3.School of Stomatology, Peking University, Beijing 100081, China
  • Received:2024-01-22 Revised:2024-05-23 Online:2025-01-01 Published:2025-01-11
  • Contact: Xiaotao Chen
  • Supported by:
    National Natural Science Foundation of China(82260195)

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摘要：

目的 分析大麻素Ⅰ型受体（CBlR）抑制或激活对大鼠硬腭黏膜创伤愈合的影响。 方法 大鼠硬腭部黏膜2 mm创伤造模，腹腔注射选择性拮抗剂Rimonabant Hydrochloride（SR141716A）和选择性激动剂Anandamide（AEA）持续7 d，记录创口平面愈合情况，组织学愈合情况，CB1R的表达水平，并通过酶联免疫吸附法检测组织匀浆的细胞因子浓度。 结果 CB1R蛋白表达水平影响硬腭黏膜创口的愈合，CB1R抑制的动物伤口出现了明显的愈合延迟，同时伴随着肿瘤坏死因子-α和单核细胞趋化蛋白-1表达升高。 结论 CB1R在大鼠硬腭黏膜创伤过程中表达于周围组织中，降低了局部组织的炎症水平，促进了伤口愈合。

关键词: 创伤愈合, 大麻素Ⅰ型受体, 硬腭黏膜

Abstract:

Objective This study aimed to explore the risk factors and prevention strategies of secondary caries in children. Methods A total of 120 children with dental caries who were re-examined 1 year after treatment in the First Affilia-ted Hospital of Zhengzhou University were divided into the secondary group (n=25) and non-secondary group (n=95). The general data, initial diagnosis data, and oral behavior data of the two groups were collected and compared. The risk factors of secondary caries in children with dental caries were analyzed by using logistic regression. Results Significant differences in age, parental education level, family income, filling materials, brushing frequency, parental help to brush teeth, mouthwash after meals, sweets intake times, regular oral review, and use of fluoride toothpaste were found between the secondary caries group and non-secondary caries group (P<0.05). Multivariate Logistic regression analysis showed that the educational level of parents, parental help to brush teeth, mouthwash after meals, brushing frequency, sweets intake times, use of fluoride toothpaste, and filling materials were independent risk factors for secondary caries in children (P<0.05). Conclusion Secondary caries in children have many risk factors. Thus, reducing secondary caries in children is important to strengthen oral health education and develop good oral health habits.

Key words: wound healing, cannabinoid type Ⅰ receptor, hard-palate mucosa

中图分类号: 

  • Q257

图 1

大鼠上腭黏膜创伤示意图和组织块样本示例A：大鼠硬腭黏膜创伤的位置和尺寸，术区消毒后用直径2 mm的穿孔活检装置损伤组织，图片为创伤后立即拍摄；B：R-H组大鼠硬腭黏膜创伤后1 d取下组织示例。"

图 2

创伤愈合过程中平面创口的闭合观察A~C：分别为Model组、R-H组和AEA组各时间点的愈合情况，采用当天创面面积和原始创面面积的百分比计算，选取了第1、3、5、7天创面的代表性图片；统计分析采用单因素方差分析，然后进行Tukey检验，***为P<0.001。"

图 3

3组大鼠3、7 d代表性图片 HE染色 × 200大鼠牙龈创伤3、7 d创伤区牙龈组织代表性HE染色图片，随着创口愈合的进行R-H组组织愈合水平明显较另外2组延迟，创伤区域大量细胞增殖。"

图 4

大鼠牙龈创伤代表性CB1R图 免疫组织化学染色 × 200"

图 5

3组大鼠CB1R的表达情况3组大鼠CB1R的免疫组织化学结果，统计分析采用单因素方差分析，然后进行Tukey检验，Model组与R-H组和AEA相比。**为P<0.01，###表示R-H组和AEA组具有显著性，P<0.001。"

图 6

3组大鼠7 d内CB1R蛋白的表达情况"

图 7

3组大鼠CB1R蛋白表达水平3组大鼠CB1R蛋白WB结果，统计分析采用单因素方差分析，然后进行Tukey检验，Model组与R-H组和AEA相比，**为P<0.01，***为P<0.001。R-H组和AEA组相比，##为P<0.01，###为P<0.001。"

图 8

采用ELISA测定Model组（左）、R-H组（中）和AEA组（右）大鼠牙龈创面组织匀浆中细胞因子含量A~C分别为TNF-α、MCP-1和IL-6的含量；统计分析采用单因素方差分析，然后进行Tukey检验，各时间点*为P<0.05，**为P<0.01，***为P<0.001，****为P<0.000 1。"

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