Int J Stomatol

Inter J Stomatol ›› 2015, Vol. 42 ›› Issue (4): 411-414.doi: 10.7518/gjkq.2015.04.009

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Expression of heat shock protein in interleukin-1β-induced injury inhuman gingival fibroblasts

Zhou Qin, Zhu Zhenkun, Sun Ruinan, Xu Xin, Ma Yue   

  1. Dept. of Oral Surgery, School of Stomatology, Shandong University; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China
  • Online:2015-07-01 Published:2015-07-01

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Abstract:

Objective This study aimed to investigate the expression of heat shock proteins(HSP27, HSP70, and HSP90) in human gingival fibroblasts(HGF) during injuries induced by interleukin(IL)-1β at different concentrations. We also aimed to investigate whether HSP expressed in tissue inflammation injury plays a key rolein inflammatory tissue damage. Methods The majority of cultured HGF were treated with serially diluted IL-1β. Wound healing assay was performed to determine the migration of HGF, and Western blotting was performed to determine the expression of HSP27, HSP70, and HSP90. Results Expression of HSP27 was positive in the IL-1β intervened HGF in a concentration-dependent manner, whereas the expression of HSP70 and HSP90 showed no obvious change. Wound healing assay showed that HGF had a lower migration capability with higher IL-1β concentration and a negative HSP27 expression quantity. Conclusion The concentration of HSP27 increased to a level that is higher than usual in IL-1β-induced injuries. HGF was positively related to cell migration, thereby suggesting that HSP27 plays an important role during the occurrence and development of wound healing. The mechanisms underlying this phenomenon require further investigation.

Key words: interleukin-1β, heat shock protein, human gingival fibroblasts, tissue inflammation


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