国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (6): 678-685.doi: 10.7518/gjkq.2018.06.010

• 论著 • 上一篇    下一篇

红景天苷对人舌鳞状细胞癌CAL-27细胞增殖、凋亡、周期及迁移的影响

王志强1,2,3,刘娅丽2,3,马丽娟1,2,杨兰1(),王若宇2,3,高舒婷1,3   

  1. 1. 兰州大学第二医院口腔颌面外科 兰州 730030
    2. 西北民族大学口腔医学国家民族事务委员会重点实验室 兰州 730030
    3. 兰州大学口腔医学院 兰州 730030
  • 收稿日期:2017-11-20 修回日期:2018-07-22 出版日期:2018-11-01 发布日期:2018-11-15
  • 通讯作者: 杨兰 E-mail:ylan2005@163.com
  • 作者简介:王志强,硕士,Email: 812248620@qq.com
  • 基金资助:
    口腔医学国家民族事务委员会重点实验室(西北民族大学)开放课题资助项目(MWZD201602)

Effects of salidroside on proliferation, apoptosis, cycle and migration of human tongue cancer CAL-27 cells

Zhiqiang Wang1,2,3,Yali Liu2,3,Lijuan Ma1,2,Lan Yang1(),Ruoyu Wang2,3,Shuting Gao1,3   

  1. 1. Dept. of Oral and Maxillofacial Surgery, The Second Hospital of Lanzhou University, Lanzhou 730030, China
    2. Key Laboratory of Stomatology of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China
    3. School of Stomatology, Lanzhou University, Lanzhou 730030, China
  • Received:2017-11-20 Revised:2018-07-22 Online:2018-11-01 Published:2018-11-15
  • Contact: Lan Yang E-mail:ylan2005@163.com
  • Supported by:
    This study was supported by Open Subject Funding Project of Key Laboratory of Stomatology, State Ethnic Affairs Commission (Northwest Minzu University)(MWZD201602)

摘要:

目的 研究红景天苷对人舌鳞状细胞癌CAL-27细胞体外增殖及迁移的影响。方法 用四甲基偶氮唑盐(MTT)法、流式细胞检测术、Western blot、Transwell小室实验等探究红景天苷对人舌鳞状细胞癌CAL-27细胞增殖、凋亡、周期及迁移的影响。结果 红景天苷对CAL-27细胞的增殖抑制作用与浓度-时间呈正相关(P<0.05);不同浓度红景天苷处理组凋亡发生率的差异均有意义(P<0.05),且与浓度呈正相关;红景天苷对CAL-27细胞周期产生显著影响,诱导其发生G0/G1期阻滞;随着红景天苷浓度的增加,CAL-27细胞迁移能力显著降低(P<0.05)。结论 红景天苷对人舌鳞状细胞癌CAL-27细胞的增殖、凋亡、周期及迁移产生影响,具有显著的体外抑制作用。

关键词: 红景天苷, 人舌鳞状细胞癌CAL-27细胞, 凋亡, 细胞增殖, 细胞周期, 细胞迁移

Abstract:

Objective To investigate the influence of salidroside on human tongue cancer cells (CAL-27) proliferation and migration.Methods 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT), flow cytometry instrument technique, Western blot and Transwell methods were used to detect the effect of salidroside for CAL-27 cell proliferation, apoptosis, cycle and migration.Results With increasing salidroside concentration and time, the influence on CAL-27 cell proliferation increased gradually, and the cell survival rate decreased obviously (P<0.05). With increasing salidroside concentration, the incidence of apoptosis of CAL-27 cell showed significant differences (P<0.05). Salidroside had a signifi-cant effect on the CAL-27 cell cycle and induced the period G0/G1 phase arrest of CAL-27 cell. With increasing salidroside concentration, CAL-27 cell migration was significantly reduced (P<0.05).Conclusion Salidroside affected proliferation, apoptosis, cycle and migration of human tongue cancer CAL-27 cells and had significant inhibitory effect in vitro.

Key words: salidroside, human tongue cancer CAL-27 cell, apoptosis, cell proliferation, cell cycle, cell migration

中图分类号: 

  • Q813.1 +1

图 1

不同浓度红景天苷作用24和48 h对CAL27细胞抑制率的影响 * P<0.05。"

图 2

不同浓度红景天苷作用于CAL-27细胞48 h Hoechst 33258荧光染色 × 100A、B、C、D、E、F:红景天苷浓度分别为0、20、40、60、80、100 μg·mL-1。"

图 3

Annexin V/PI荧光探针和流式细胞仪检测不同浓度红景天苷作用48 h对CAL-27细胞凋亡的影响** P<0.05。"

表 1

流式细胞术检测不同浓度红景天苷作用48 h诱导CAL-27细胞周期变化"

红景天苷浓度/(μg·mL-1 不同细胞周期的细胞比例/%
G0/G1 S期 G2/M期
20 45.03 28.22 0.83
40 62.99 25.37 0.12
60 72.76 21.32 0.16
80 76.96 15.50 0.06
100 81.28 14.82 0

图 4

不同浓度红景天苷对CAL-27细胞24 h迁移能力的影响 A、B、C、D、E、F:红景天苷浓度分别为0、20、40、60、80、100 μg·mL-1。*P<0.05,**P<0.01。"

图 5

不同浓度红景天苷对CAL-27细胞蛋白质表达的影响 *P<0.05。"

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