国际口腔医学杂志 ›› 2014, Vol. 41 ›› Issue (1): 23-25.doi: 10.7518/gjkq.2014.01.006

• 论著 • 上一篇    下一篇

舌癌Tca8113细胞来源exosomes的制备及鉴定

李金1 廖贵清2 陈巨峰1 刘海潮2 苏宇雄2 冯炼强3 骆晓枫4   

  1. 1.中山大学附属佛山第一人民医院口腔科 佛山 528000; 2.中山大学光华口腔医学院•附属口腔医院口腔颌面外科 广州 510055; 3.中山大学中山医学院免疫教研室;4.生物化学教研室 广州 510080
  • 收稿日期:2013-06-28 修回日期:2013-10-21 出版日期:2014-01-01 发布日期:2014-01-01
  • 通讯作者: 廖贵清,主任医师,博士,Email:drliaoguiqing@hotmail.com
  • 作者简介:李金,主治医师,硕士,Email:lijin99655@163.com
  • 基金资助:

    国家自然科学基金(30572062);广东省自然科学基金(5001720)

Isolation and identification of exosomes secreted from cell line Tca8113 in tongue cancer

Li Jin1, Liao Guiqing2, Chen Jufeng1, Liu Haichao2, Su Yuxiong2, Feng Lianqiang3, Luo Xiaofeng4.   

  1. 1. Dept. of Stomatology, The First Hospital of Foshan, Foshan 528000, China; 2. Dept. of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou 510055, China; 3. Dept. of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; 4. Dept. of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
  • Received:2013-06-28 Revised:2013-10-21 Online:2014-01-01 Published:2014-01-01

摘要:

目的 探讨舌癌Tca8113细胞能否分泌exosomes,为舌癌的免疫治疗寻找新方法。方法 采用超速离心及差异密度梯度离心法,从Tca8113细胞中分离并纯化exosomes,通过透射电子显微镜观察其超微结构,蛋白质印迹法分析其表面蛋白成分。结果 从Tca8113细胞中成功分离并纯化出exosomes,其结构及携带蛋白与其他细胞来源的相似,并发现其分泌的exosomes还携带有肿瘤抗原。结论 舌癌Tca8113细胞能分泌exosomes,而且其携带多种与肿瘤免疫相关的蛋白可能具有抗肿瘤作用。

关键词: exosomes, 蛋白质印迹法, 透射电子显微镜, 肿瘤免疫

Abstract:

Objective This study aims to verify whether Tca8113 can secrete exosomes, and to identify a new method for oral tumor immunotherapy. Methods Exosomes were isolated and purified from the culture supernatant of Tca8113 by ultraspeed centrifugation and density gradient centrifugation, and were subsequently confirmed using transmission electron microscopy and Western blot. Results Exosomes were successfully isolated and purified from the culture supernatant of Tca8113. Tca8113 contained protein and had a structure similar to other cells, as reported in previous studies. Conclusion Tca8113 can secrete exosomes, and contains numerous types of proteins that are associated with tumor immunity.

Key words: exosomes, western blot, transmission electron microscopy, tumor immunity

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