%A Wang Xiaojing, Zhu Zhuoli, Yu Haiyang %T Proliferation and differentiation of the vascular endothelial cell under microvibration %0 Journal Article %D 2013 %J Int J Stomatol %R 10.7518/gjkq.2013.03.003 %P 288-290 %V 40 %N 3 %U {https://www.gjkqyxzz.cn/CN/abstract/article_9081.shtml} %8 2013-05-01 %X

Objective To evaluate the proliferation and differentiation of human umbilical vein endothelial cell(HUVEC) under microvibration. Methods HUVECs were randomly divided into three groups: Group A was blank control group, group B was vibrated under 80 Hz, 30 min per day, group C was vibrated under 40 Hz, 30 min per day; observed HUVEC proliferations based on micro-vibration duration on 0, 3, 5, 7 d. Methyl thiazolyl tetrazolium(MTT) assay was used to detect cell proliferation and growth curve. According to the result of the growth curve, we took another HUVECs divided into 4 groups:Group a was vibrated immediately, group b was blank control of group a, group c was vibrated during three days, group d was blank control of group c. Western blot assay was used to detect secretion of intercellular adhesion molecule(ICAM)-1, endothelial nitric oxide synthase(eNOS) and phosphorylate-eNOS. Results MTT growth curve showed the proliferation mainly from the first to the third day, stepped into the plateau period from the fifth day. Group C proliferated more quickly than in group B from the first day, more quickly than group A from the third day, proliferation capacity of group B was always lower than group A, and the differences were statistically significant(P<0.05). The expressions of ICAM-1 and PeNOS in group c were the highest in all groups. Conclusion The low amplitude high frequency microvibration promoted ICAM-1 and P-eNOS expression, enhanced cell adhesion.