Inter J Stomatol ›› 2017, Vol. 44 ›› Issue (4): 411-420.doi: 10.7518/gjkq.2017.04.009

• Original Articles • Previous Articles     Next Articles

Biofilm formation and antimicrobial susceptibility of Streptococcus mitis in response to luxS/AI-2 signaling

Lu Xiao1, Weng Chunhui1, Wang Jinming2, Liu Shaojuan1, Liu Qin1, Lin Shan1   

  1. 1. Dept. of Stomatology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, China;
    2. Guanghua School of Stomatology, Dept. of Implantation, Hospital of Stomatology, Sun Yat-sen University; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
  • Received:2016-07-22 Revised:2017-02-20 Online:2017-07-01 Published:2017-07-01
  • Supported by:
    This study was supported by Science and Technology Program of Guangzhou(2011J4100036).

Abstract: Objective This paper aims to study the influence of luxS gene mutation of Streptococcus mitis on biofilm formation and antimicrobial susceptibility. Methods Culture in blood plate, Gram stain and susceptibility tests, and phy-siological and biochemical tests were first carried out. 16S ribosomal DNA(rDNA) sequence homology analysis of clinical isolate was examined subsequently. The autoinducer-2(AI-2) bioluminescence assay of Vibrio harveyi BB170 strain, multi-drug resistance, and ability of biofilm formation were examined in luxSmutant and clinical isolate. The structure, exopo-lysaccharides, and dead/live cells of biofilm were scanned by confocal laser scanning microscope. Compared with one another, luxS mutant growth recruited by isolate supernatant or 4,5-dihydroxy-2,3-pentanedione(DPD) was experimented. Results luxS gene deletion decreased biofilm formation(P<0.001), loosened structure, and changed antimicrobial suscepti-bility(to ampicillin, ciprofloxacin, tetracycline). However, isolate supernatant or DPD can recover the structure and ability of biofilm formation in luxS mutant. Conclusion luxS/AI- 2 signaling can influence biofilm formation and antimicro-bial susceptibility of S. mitis with multidrug resistance.

Key words: luxS gene, autoinducer-2, quorum sensing, biofilm, antimicrobial susceptibility

CLC Number: 

  • R73

TrendMD: 
[1] 李金钟. 链球菌分类与鉴定新进展[J]. 临床检验杂志, 2006, 24(5):391-393.
Li JZ. New progress in classification and identifica-tion of Streptococcus [J]. Chin J Clin Lab Sci, 2006, 24(5):391-393.
[2] Junka AF, Szymczyk P, Smutnicka D, et al. Micro-bial biofilms are able to destroy hydroxyapatite in the absence of host immunity in vitro [J]. J Oral Max-illofac Surg, 2015, 73(3):451-464.
[3] 王丽娟, 缪羽, 赵峰. 牙周炎与冠心病相关性的研究进展[J]. 中华老年口腔医学杂志, 2013, 11(1): 47-50.
Wang LJ, Miu Y, Zhao F. Research on the relation-ship between periodontitis and coronary heart dis-ease[J]. Chin J Geriat Dent, 2013, 11(1):47-50.
[4] 李仲兴. 关注缓症链球菌及其感染[J]. 中国感染控制杂志, 2007, 6(3):145-148.
Li ZX. Pay close attention to Streptococcus mitis and its infection[J]. Chin J Infect Control, 2007, 6(3): 145-148.
[5] Collins J R, Arredondo A, Roa A, et al. Periodontal pathogens and tetracycline resistance genes in sub-gingival biofilm of periodontally healthy and dis-eased Dominican adults[J]. Clin Oral Investig, 2016, 20(2):349-356.
[6] 李光辉, 朱德妹, 汪复, 等. 2012年中国CHINET血培养临床分离菌的分布及耐药性[J]. 中国感染与化疗杂志, 2014, 14(6):474-481.
Li GH, Zhu DM, Wang F, et al. The distribution and antibiotic resistance of clinical isolates from blood culture in 2012 CHINET surveillance program in China[J]. Chin J Infect Chemother, 2014, 14(6):474- 481.
[7] 苗棣, 张晖, 徐屹. 牙周致病菌对各类抗菌药物敏感性和耐药性的研究进展[J]. 国际口腔医学杂志, 2008, 35(4):383-386.
Miao D, Zhang H, Xu Y. Review on the suscepti-bility and antimicrobial resistance of periodontal pathogenes to antimicrobial agents[J]. Int J Stomatol, 2008, 35(4):383-386.
[8] 吴梦晖. 缓症链球菌肺炎的药物敏感试验分析[J]. 中国医药指南, 2012, 10(22):21-22.
Wu MH. Sensitivity analysis of 189 cases of Stre-ptococcus mitis pneumonia[J]. Guid Chin Med, 2012, 10(22):21-22
[9] Chun S, Huh HJ, Lee NY. Species-specific difference in antimicrobial susceptibility among viridans group streptococci[J]. Ann Lab Med, 2015, 35(2):205-211.
[10] Garg N, Manchanda G, Kumar A. Bacterial quorum sensing: circuits and applications[J]. Antonie Van Leeuwenhoek, 2014, 105(2):289-305.
[11] Pereira CS, Thompson JA, Xavier KB. AI-2-media-ted signalling in bacteria[J]. FEMS Microbiol Rev, 2013, 37(2):156-181.
[12] Wang Y, Yi L, Zhang Z, et al. Biofilm formation, host-cell adherence, and virulence genes regulation of Streptococcus suis in response to autoinducer-2 signaling[J]. Curr Microbiol, 2014, 68(5):575-580.
[13] Chen X, Schauder S, Potier N, et al. Structural iden-tification of a bacterial quorum-sensing signal con-taining Boron[J]. Nature, 2002, 415(6871):545-549.
[14] 张耀超, 韩育植, 韩福胜. 变异链球菌 LuxS 基因缺陷突变株的构建及其生物膜结构的初步观察研究[J]. 牙体牙髓牙周病学杂志, 2012, 22(6):309-313.
Zhang YC, Han YZ, Han FS. Construction of LuxS -deleted Streptococcus mutans and biofilm formation property of the LuxS -deleted strain[J]. Chin J Con-serv Dent, 2012, 22(6):309-313.
[15] 于丹妮, 韩福胜, 韩玉植, 等. LuxS 基因缺失的变形链球菌突变株的构建及鉴定[J]. 中华微生物学和免疫学杂志, 2008, 28(3):198-202.
Yu DN, Han FS, Han YZ et al. Construction and identification of a LuxS -deleted mutant strain of S.mutans [J]. Chin J Microbiol Immunol, 2008, 28(3): 198-202.
[16] Surette MG, Miller MB, Bassler BL. Quorum sensing in Escherichia coli , Salmonella typhimurium , and Vibrio harveyi : a new family of genes responsi-ble for autoinducer production[J]. Proc Natl Acad Sci USA, 1999, 96(4):1639-1644.
[17] 李焕平, 王凌峰, 赵子龙. 细菌生物膜的相关性研究[J]. 包头医学院学报, 2014(3):146-148.
Li HP, Wang LF, Zhao ZL. Research about bacterial biofilm[J]. J Baotou Med Col, 2014(3):146-148.
[18] Rozen R, Bachrach G, Zachs B et al. Growth rate and biofilm thickness of Streptococcus sobrinus and Streptococcus mutans on hydroxapatite[J]. APMIS, 2001, 109(2):155-160.
[19] Bassler BL, Greenberg EP, Stevens AM. Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi [J]. J Bacteriol, 1997, 179(12):4043-4045.
[20] Merritt J, Qi F, Goodman SD, et al. Mutation of luxS affects biofilm formation in Streptococcus mutans [J]. Infect Immun, 2003, 71(4):1972-1979.
[21] Ahmed NA, Petersen FC, Scheie AA. Biofilm for-mation and autoinducer-2 signaling in Streptoco-ccus intermedius : role of thermal and pH factors[J]. Oral Microbiol Immunol, 2008, 23(6):492-497.
[22] Ahmed NA, Petersen FC, Scheie AA. AI-2/LuxS is involved in increased biofilm formation by Strepto-coccus intermedius in the presence of antibiotics[J]. Antimicrob Agents Chemother, 2009, 53(10):4258- 4263.
[23] Pecharki D, Petersen FC, Scheie AA. LuxS and ex-pression of virulence factors in Streptococcus inter-medius [J]. Oral Microbiol Immunol, 2008, 23(1):79- 83.
[24] Cuadra-Saenz G, Rao DL, Underwood AJ, et al. Au- toinducer-2 influences interactions amongst pioneer colonizing streptococci in oral biofilms[J]. Microbio-logy, 2012, 158(Pt 7):1783-1795.
[25] Mcnab R, Ford SK, El-Sabaeny A, et al. LuxS-based signaling in Streptococcus gordonii : autoinducer 2 controls carbohydrate metabolism and biofilm for-mation with Porphyromonas gingivalis [J]. J Bacte-riol, 2003, 185(1):274-284.
[26] Kozlova EV, Popov VL, Sha J, et al. Mutation in the S-ribosylhomocysteinase(luxS) gene involved in quorum sensing affects biofilm formation and viru-lence in a clinical isolate of Aeromonas hydrophila [J]. Microb Pathog, 2008, 45(5/6):343-354.
[27] 张耀超. LuxS 基因缺失对变异链球菌生物学性状的影响[J]. 牙体牙髓牙周病学杂志, 2012, 22(9): 499-503.
Zhang YC. LuxS gene mutation affects biological characteristics and biofilm formation of Streptococcus mutans [J]. Chin J Conserv Dent, 2012, 22(9):499- 503.
[28] Xiao J, Koo H. Structural organization and dynamics of exopolysaccharide matrix and microcolonies for-mation by Streptococcus mutans in biofilms[J]. J Appl Microbiol, 2010, 108(6):2103-2113.
[29] 黄晓晶, 江山, 蔡志宇, 等. 生物膜状态变异链球菌临床株合成胞外多糖能力的研究[J]. 重庆医科大学学报, 2011, 36(3):281-285.
Huang XJ, Jiang S, Cai ZY, et al. The difference of the ability to synthesize exopolysaccharides of clin-ical strains of biofilm Streptococcus mutans [J]. J Chongqing Med Univ, 2011, 36(3):281-285.
[30] 何智妍, 王玉霞, 黄正蔚. 变形链球菌 luxS 基因高表达对生物膜形成的影响[J]. 中华口腔医学杂志, 2015, 50(9):554-560.
He ZY, Wang YX, Huang ZW. Effect of luxS ove-rexpression on biofilm formation by Streptococcus mutans [J]. Chin J Stomatol, 2015, 50(9):554-560.
[31] 王倩, 唐子圣, 马瑞, 等. 变异链球菌 luxS 基因对多糖基质代谢的影响[J]. 上海口腔医学, 2011, 20(1): 6-9.
Wang Q, Tang ZS, Ma R, et al. Influence of Strepto-coccus mutans luxS gene on polysaccharide matrix metabolism[J]. Shanghai J Stomatol, 2011, 20(1):6- 9.
[32] Ahmed NA, Petersen FC, Scheie AA. AI-2 quorum sensing affects antibiotic susceptibility in Streptoco-ccus anginosus [J]. J Antimicrob Chemother, 2007, 60(1):49-53.
[33] Silva JO, Martins Reis AC, Quesada-Gómez C, et al. In vitro effect of antibiotics on biofilm formation by Bacteroides fragilis group strains isolated from intestinal microbiota of dogs and their antimicrobial susceptibility[J]. Anaerobe, 2014, 28:24-28.
[34] Suntharalingam P, Cvitkovitch DG. Quorum sensing in streptococcal biofilm formation[J]. Trends Micro-biol, 2005, 13(1):3-6.
[35] 徐蓉蓉, 王斌, 葛久禹. 口腔链球菌密度感应信号系统 comE 基因及 luxS 基因的检测分析[J]. 华西口腔医学杂志, 2011, 29(4):355-357.
Xu RR, Wang B, Ge JY. Detection and analysis of comE and luxS genes in quorum sensing signal pathway from Streptococcus oralis [J]. West China J Stomatol, 2011, 29(4):355-357.
[36] 陈娇. 双组分信号传导系统与变异链球菌的致病相关特性[J]. 国际口腔医学杂志, 2011, 38(3): 297- 300.
Chen J. Two-component signal transduction system and the virulence-related characteristics of Streptoco-ccus mutans [J]. Int J Stomatol, 2011, 38(3):297-300.
[1] Yang Sirui,Ren Biao,Peng Xian,Xu Xin. Research progress on drug synergism with fluconazole in fluconazole-resistant Candida albicans [J]. Int J Stomatol, 2022, 49(5): 511-520.
[2] Li Shanshan,Yang Fang. Research progress on the relationship between Streptococcus mutans and Candida albicans in caries [J]. Int J Stomatol, 2022, 49(4): 392-396.
[3] Huang Peiqing,Peng Xian,Xu Xin. Volatile sulfur compounds and its role in the management of intra-oral halitosis [J]. Int J Stomatol, 2021, 48(5): 592-599.
[4] Li Shijia,Chen Qiuyu,Zou Jing,Huang Ruijie. Effect of nicotine on the growth of oral bacteria in single or mixed species [J]. Int J Stomatol, 2021, 48(3): 305-311.
[5] Zi Yang,Benxiang Hou. Research progress on the extra- and intra-radicular biofilm associated with persistent apical periodontitis [J]. Inter J Stomatol, 2019, 46(2): 238-243.
[6] Fang Hongzhi, Tian Yuanyuan, Yu Xuan, Yang Yingming, Yang Hui, Hu Tao. The influence of exogenous dextranase and sodium fluoride on biofilm of Actinomyces viscosus under different sucrose concentration conditions [J]. Inter J Stomatol, 2017, 44(6): 654-659.
[7] Liu Shiyu, Tian Mi, Shi Liran, Pan Weilin, Wang Yiyao, Li Mingyun. Effect of nicotine and mecamylamine on growth of periodontal pathogens [J]. Inter J Stomatol, 2017, 44(4): 421-425.
[8] Zhou Shuangshuang, Zheng Xin, Zhou Xuedong, Xu Xin. Relationship of alkali production by plaque biofilm and dental caries [J]. Inter J Stomatol, 2016, 43(5): 573-577.
[9] Zhang Ying, Li Mingyong, Huo Li, Meng Yuan. Biosynthesis of autoinducer-2 and determination of its bioactivity in vitro [J]. Inter J Stomatol, 2016, 43(5): 519-523.
[10] Liu Yi1, Fei Wei1, Wang Lina2, Zhang Siyu3, Wang Yanjun1, Wu Hongkun4.. Effects of synthetic antimicrobial decapeptide on the growth and structure of Streptococcus mutans biofilm [J]. Inter J Stomatol, 2015, 42(4): 401-405.
[11] Ou Meizhen, Ling Junqi. Multiple effect of polyamines on biofilm [J]. Inter J Stomatol, 2015, 42(3): 361-363.
[12] Wang Haohao, Cheng Lei. Effects of oral restorative materials on biofilms [J]. Inter J Stomatol, 2015, 42(3): 352-356.
[13] Ou Meizhen, Ling Junqi. Effects of D-type amino acids on biofilm dissociation [J]. Inter J Stomatol, 2015, 42(2): 203-205.
[14] Zhang Jianying, Ling Junqi. Function of surface protein antigen P in the biofilm formation of Streptococcus mutans [J]. Inter J Stomatol, 2015, 42(1): 111-113.
[15] Geng Fengxue, Pan Yaping.. Interactions among bacteria of different colonization processes within biofilms and the model [J]. Inter J Stomatol, 2014, 41(4): 431-435.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[7] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[8] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[9] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[10] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .