Inter J Stomatol ›› 2013, Vol. 40 ›› Issue (3): 288-290.doi: 10.7518/gjkq.2013.03.003

Previous Articles     Next Articles

Proliferation and differentiation of the vascular endothelial cell under microvibration

Wang Xiaojing, Zhu Zhuoli, Yu Haiyang   

  1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University,Chengdu 610041, China
  • Received:2013-01-15 Revised:2013-02-28 Online:2013-05-01 Published:2013-05-01

Abstract:

Objective To evaluate the proliferation and differentiation of human umbilical vein endothelial cell(HUVEC) under microvibration. Methods HUVECs were randomly divided into three groups: Group A was blank control group, group B was vibrated under 80 Hz, 30 min per day, group C was vibrated under 40 Hz, 30 min per day; observed HUVEC proliferations based on micro-vibration duration on 0, 3, 5, 7 d. Methyl thiazolyl tetrazolium(MTT) assay was used to detect cell proliferation and growth curve. According to the result of the growth curve, we took another HUVECs divided into 4 groups:Group a was vibrated immediately, group b was blank control of group a, group c was vibrated during three days, group d was blank control of group c. Western blot assay was used to detect secretion of intercellular adhesion molecule(ICAM)-1, endothelial nitric oxide synthase(eNOS) and phosphorylate-eNOS. Results MTT growth curve showed the proliferation mainly from the first to the third day, stepped into the plateau period from the fifth day. Group C proliferated more quickly than in group B from the first day, more quickly than group A from the third day, proliferation capacity of group B was always lower than group A, and the differences were statistically significant(P<0.05). The expressions of ICAM-1 and PeNOS in group c were the highest in all groups. Conclusion The low amplitude high frequency microvibration promoted ICAM-1 and P-eNOS expression, enhanced cell adhesion.

Key words: microvibration, human umbilical vein endothelial cell, proliferation


TrendMD: 
[1] Zhou Jinkuo,Zhang Jinhong,Shi Xiaojing,Liu Guangshun,Jiang Lei,Liu Qianfeng. Influences of long noncoding RNA small nucleolar RNA host gene 22 on the cell proliferation, invasion and migration of oral squamous carcinoma cells by regulating microRNA-27b-3p [J]. Int J Stomatol, 2024, 51(1): 52-59.
[2] Xiong Menglin,Wu Long,Ma Li,Zhao Jin. Role of transforming growth factor-β2 in promoting the proliferation and differentiation of dental pulp stem cells [J]. Int J Stomatol, 2021, 48(6): 635-639.
[3] Liu Juan,Chen Bin,Yan Fuhua. Effects of platelet-rich plasma and concentrated growth factor on the proliferation and osteogenic differentiation of human periodontal cells [J]. Int J Stomatol, 2021, 48(5): 520-527.
[4] Li Huili,Fang Changyun,Su Zheng. Effects of arecoline on proliferation and migration of human buccal mucosal fibroblasts in vitro [J]. Int J Stomatol, 2020, 47(1): 32-36.
[5] Zeng Kan,Li Xin,Wang Chenglin,Yang Jing,Ye Ling. Effects of bone microenvironment cells on tumor bone metastasis [J]. Int J Stomatol, 2020, 47(1): 95-101.
[6] Mei Hongxiang,Zhang Yidan,Zhang Chenghao,Liu Enyan,Chen Hao,Zhao Zhihe,Liao Wen. Effect of epigallocatechin-3-gallate on stem cell proliferation and osteogenic differentiation [J]. Int J Stomatol, 2019, 46(4): 431-436.
[7] Yuxuan Yang,Haixia Zhang,Shuang Wang. Biological function of amelogenin during periodontal regeneration [J]. Inter J Stomatol, 2019, 46(2): 191-196.
[8] Yuanyuan Li,Bin Cheng,Yun Wang. Effects of long non-coding RNA lnc-p26090 on the glycolysis and proliferation in oral squamous cell carcinoma [J]. Inter J Stomatol, 2018, 45(6): 628-634.
[9] Zhiqiang Wang,Yali Liu,Lijuan Ma,Lan Yang,Ruoyu Wang,Shuting Gao. Effects of salidroside on proliferation, apoptosis, cycle and migration of human tongue cancer CAL-27 cells [J]. Inter J Stomatol, 2018, 45(6): 678-685.
[10] Zhan Yeming, Zhang Mingzhu. Research progress on the relevance between drug-induced gingival overgrowth and cell proliferation and apoptosis [J]. Inter J Stomatol, 2018, 45(2): 199-203.
[11] Wang Qi, Chen Xiyan, Wen Yong.. Research progress on the crosstalk between Hippo/YAP signaling pathway and cell proliferation-related signaling pathways [J]. Inter J Stomatol, 2017, 44(5): 614-618.
[12] Cheng Qun, Yang Minghua, Chen Bin, Liu Juan,Yan Fuhua. Effect of Er:YAG laser irradiation on the cell proliferation and migration of human periodontal ligament cells [J]. Inter J Stomatol, 2015, 42(2): 135-139.
[13] He Kun1, Cheng Xiangrong2, Zhang Ximu3. Influence of enamel matrix protein on attachment and proliferation ability of dental follicle cells and periodontal ligament cells cultured on titanium [J]. Inter J Stomatol, 2014, 41(5): 536-540.
[14] Ji Qiuxia, Yuan Changqing, Song Wenbin, Wu Hong.. Effects of quaternized chitosan on the cell proliferation and cell cycle of human periodontal ligament cells [J]. Inter J Stomatol, 2013, 40(5): 588-591.
[15] Zhang Shu, Wang Jing, Lin Yunfeng.. Research progress on the effects of surface microtopography on cell behavior [J]. Inter J Stomatol, 2013, 40(4): 544-546.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[7] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[8] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[9] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[10] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .