Int J Stomatol

Int J Stomatol ›› 2022, Vol. 49 ›› Issue (4): 412-419.doi: 10.7518/gjkq.2022068

• Original Articles • Previous Articles     Next Articles

In vitro cytotoxicity of composite resin and compomer and effect on osteogenic differentiation of osteoblasts

Zhang Jingyi1(),Li Danwei1(),Sun Yu2,Lei Yayan1,Liu Tao1,Gong Yu1   

  1. 1.Dept. of Cariology and Endodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650106, China
    2.Comfort Treatment Center, Kunming Blue Orange Stomatological Hospital, Kunming 650228, China
  • Received:2021-11-02 Revised:2022-04-19 Online:2022-07-01 Published:2022-06-28
  • Contact: Danwei Li E-mail:531345553@qq.com;89402424@qq.com
  • Supported by:
    Scientific Research Fund Project of Education Department of Yunnan Province(2018JS234)

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Abstract:

Objective To explore a new method for repairing root defects by observing the osteoblast toxicity of composite resin and compomer, as well as their effects on osteogenic differentiation. Methods An experimental model of MC3T3-E1 was established for osteogenesis induction in vitro. Scanning electron microscope (SEM), cell counting kit-8 (CCK-8) detection, apoptosis and cell cycle detection, scratch experiment, alkaline phosphatase (ALP) activity detection, Alizarin red staining, and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to compare the biological effects of the two materials on MC3T3-E1 cells. Results The MC3T3-E1 cells adhered to the two kinds of materials well. The composite resin extract showed slightly higher cytotoxicity to MC3T3-E1 but could promote cell migration and upregulate osteocalcin (OCN), osteopontin (OPN), runt-related transcription factor 2 (RUNX2), and collagen 1 (COL-1) mRNA expression. The compomer extract significantly inhibited the expression of osteogenic differentiation-related genes (P<0.05). Conclusion The slight cytotoxicity of the composite resin and compomer did not considerably affect the adhesion, growth, and normal differentiation of osteoblasts; thus, both materials have potential as bone replacement materials.

Key words: composite resin, compomer, osteoblast, cytotoxicity, osteogenic differentiation

CLC Number: 

  • Q 291

TrendMD: 

Tab 1

PCR primer sequences"

名称引物序列
OCN上游GGACCATCTTTCTGCTCACTCTGC
下游TCCTGCTTGGACATGAAGGCTTTG
OPN上游AAACACACAGACTTGAGCATTC
下游TTAGGGTCTAGGACTAGCTTGT
RUNX2上游CCTTCAAGGTTGTAGCCCTC
下游GGAGTAGTTCTCATCATTCCCG
COL-1上游TGAACGTGGTGTACAAAGGTC
下游CCATCTTTACCAGGAGAACCAT

Fig 1

The adhesion of MC3T3-E1 cells on the surface of the composite resin and the compomer SEM"

Fig 2

Effect of different concentration Spectrum composite resin and Dyract XP compomer extracts on MC3T3-E1 proliferation"

Fig 3

Effect of Spectrum composite resin and Dyract XP compomer extracts on the apoptosis rate of MC3T3-E1 cells in 48 hours"

Fig 4

Effect of Spectrum composite resin and Dyract XP compomer extracts on the cell cycle ratio of MC3T3-E1 cells in 48 hours"

Fig 5

Effect of Spectrum composite resin and Dyract XP compomer extracts on the migration ability of MC3T3-E1 cells"

Fig 6

Effect of Spectrum composite resin and Dyract XP compomer extracts on the formation of mineralized nodules in MC3T3-E1 cells in 7 days"

Fig 7

Effect of Spectrum composite resin and Dyract XP compomer extracts on ALP activity of MC3T3-E1 cells"

Fig 8

Effects of Spectrum composite resin and Dyract XP compomer extracts on the secretion of osteogenesis related genes in MC3T3-E1 cells"

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