Int J Stomatol

Int J Stomatol ›› 2020, Vol. 47 ›› Issue (3): 278-285.doi: 10.7518/gjkq.2020061

• Original Articles • Previous Articles     Next Articles

Effects of inorganic bovine bone treated with low temperature argon-oxygen plasma on the adhesion, proliferation, and differentiation of MC3T3-E1 cells

Ma Kai,Li Hao,Zhao Hongmei,Wang Yongliang,Liu Jie,Bai Na()   

  1. Dept. of Prosthodontics, The Affiliated Hospital of Qingdao University, School of Stomatology of Qingdao University, Qingdao 266003, China
  • Received:2019-10-22 Revised:2020-01-06 Online:2020-05-01 Published:2020-05-08
  • Contact: Na Bai E-mail:bainafirst@163.com
  • Supported by:
    National Natural Science Foundation of China(81500882)

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Abstract:

Objective To study the adhesion, proliferation, and differentiation of mouse embryonic osteoblast MC3T3-E1 on inorganic bovine bone treated with low temperature argon-oxygen plasma. Methods After the surface activation of inorganic bovine bone by low temperature argon-oxygen plasma, the surface morphology of inorganic bovine bone before and after treatment was observed by scanning electron microscopy (SEM), and the surface elemental composition was detected by X-ray photoelectron spectroscopy. The MC3T3-E1 cells were inoculated on the surface of inorganic bovine bone treated with low temperature argon-oxygen plasma, and the cell adhesion morphology was observed by SEM. The proliferation of cells on days 1, 3, and 5 was detected using the CCK-8 method, and the differentiation of cells on days 7 and 14 was detected using the alkaline phosphatase (ALP) method. The control group comprised the inorganic bovine bone without treatment. Results No significant change was observed in the surface morphology of the inorganic bovine bone of the control and the argon-oxygen groups. Relative to the composition of the material elements in the control group, the argon-oxygen group had decreased surface C element and increased O, Ca, and P elements. Under SEM, the cell adhesion of the argon-oxygen group was more complete, and the cell extended pseudopodia. The cell proliferation test results showed that the number of cell proliferation in the argon-oxygen group was significantly higher than that in the control group at days 1, 3, and 5. The cell differentiation results showed that the ALP activity in the argon-oxygen group was higher than that in control group at day 14. Conclusion The inorganic bovine bone treated with low temperature argon-oxygen plasma can promote the adhesion, proliferation, and differentiation of mouse embryonic osteoblast cells.

Key words: low temperature plasma, inorganic bovine bone, mouse embryonic osteoblast MC3T3-E1, guided bone regeneration

CLC Number: 

  • Q25

TrendMD: 

Fig 1

Schematic diagram of low temperature argon-oxygen plasma system"

Fig 2

The relationship between treatment time of low temperature ar-gon-oxygen plasma system and surface temperature of materials"

Fig 3

The excessive temperature caused the fracture of the internal connecting structure of the inorganic bone SEM × 2 000"

Fig 4

The surface morphology of the experimental group and control group SEM"

Tab 1

XPS analysis of the percentage of bone surface elements in two groups %"

组别 C1s O1s P2p Ca2p
对照 50.92 37.05 5.94 6.10
实验 43.09 43.11 6.72 7.08

Fig 5

XPS detected changes in bone surface elements in the two groups"

Fig 6

The percentage of surface elements in the experimental group after immediate and 2 days of exposure"

Fig 7

MC3T3-E1 cell morphology on the surface of the control group and the experimental group SEM"

Fig 8

Percentage of adherent surface area of osteoblasts in the experi-mental group and control group"

Fig 9

Proliferation of MC3T3-E1 cells"

Fig 10

ALP activity of MC3T3-E1 cells"

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