Int J Stomatol

Int J Stomatol ›› 2020, Vol. 47 ›› Issue (1): 32-36.doi: 10.7518/gjkq.2020027

• Original Articles • Previous Articles     Next Articles

Effects of arecoline on proliferation and migration of human buccal mucosal fibroblasts in vitro

Li Huili1,Fang Changyun2(),Su Zheng3   

  1. 1. Dept. of Pediatric Dentistry, Stomatological Hospital Affiliated to Xiamen Medical College, Xiamen 361003, China
    2. Dept. of Stomatology, Xiangya Hospital, Central South University, Changsha 410078, China
    3. Dept. of Endodontics, Hospital of Stomatology, Capital Medical University, Beijing 100050, China
  • Received:2019-04-08 Revised:2019-09-21 Online:2020-01-01 Published:2020-01-01
  • Contact: Changyun Fang E-mail:biodenti@gmail.com

RichHTML

14

PDF (PC)

178

Abstract:

Objective The role of arecoline in the pathogenesis of oral submucous fibrosis (OSF) was explored by investigating the effects of arecoline on the cell proliferation, migration and filament actin of human buccal mucosal fibroblasts in vitro. Methods The methyl thiazolyl tetrazolium (MTT) assay, wound healing assay and laser scanning confocal microscopy were used to detect the effect of arecoline at different concentration (5, 10, 20, 40, 80 µg·mL-1) for human buccal mucosal fibroblasts proliferation, migration and filament actin. Results Cell proliferation, migration and polymerization of filament actin of human buccal mucosal fibroblasts in vitro were enhanced by arecoline at lower concentrations (5, 10 µg·mL-1) (P＜0.05), and inhibited by arecoline at higher concentrations (20, 40, 80 µg·mL-1) (P＜ 0.05). Conclusion Arecoline may change the proliferation and migration of human buccal mucosal fibroblasts through polymerization of filament actin.

Key words: arecoline, filament actin, proliferation, migration

CLC Number: 

  • R781.5

TrendMD: 

Fig 1

Negative expression of anti-keratin immunohistochemical staining × 100"

Fig 1

Positive expression of anti-fibroin immunohistochemical staining × 100"

Tab 1

Effect of arecoline on the proliferation of hu- man buccal mucosal fibroblasts"

浓度/
(µg·mL-1)		 作用时间/h
12 24 48 72
0 0.080±0.005 0.150±0.027 0.230±0.021 0.348±0.021
5 0.060±0.006 0.186±0.001 0.273±0.006* 0.312±0.021*
10 0.068±0.004 0.194±0.006* 0.275±0.021* 0.319±0.008*
20 0.070±0.003 0.178±0.003* 0.190±0.016* 0.330±0.009*
40 0.079±0.012 0.130±0.002* 0.186±0.004* 0.292±0.006*
80 0.073±0.018 0.078±0.016* 0.077±0.017* 0.218±0.036*

Fig 3

Effect of arecoline on the proliferation of human buccal mucosal fibroblasts"

Fig 4

Effect of arecoline on the migration of human buccal mucosal fibroblasts"

Fig 5

The filament actin of cells after immunofluorescence stain × 200"

[1] Zdrojewicz Z, Kosowski W, Królikowska N , et al. Betel—the fourth most popular substance in the world[J]. Pol Merkur Lekarski, 2015,39(231):181-185.
[2] Zhang SS, Li WH, Gao YJ , et al. Betel-quid and oral submucous fibrosis: a cross-sectional study in Hunan province, China[J]. J Oral Pathol Med, 2012,41(10):748-754.
[3] Lee CH, Ko YC, Huang HL , et al. The precancer risk of betel quid chewing, tobacco use and alcohol consumption in oral leukoplakia and oral submucous fibrosis in southern Taiwan[J]. Br J Cancer, 2003,88(3):366-372.
[4] 刘健, 凌天牗, 傅润英 , 等. 槟榔碱对HaCaT细胞增殖活性及S100A7 mRNA表达的影响[J]. 口腔医学研究, 2010,26(5):664-666, 670.
Liu J, Ling TY, Fu RY , et al. Effect of arecoline on proliferation and expression of S100A7 mRNA in HaCaT[J]. J Ora1 Sci Res, 2010,26(5):664-666, 670.
[5] Tseng SK, Chang MC, Su CY , et al. Arecoline induced cell cycle arrest, apoptosis, and cytotoxicity to human endothelial cells[J]. Clin Oral Investig, 2012,16(4):1267-1273.
[6] 凌宏艳, 杨丝丝, 李兴 , 等. 槟榔碱对3T3-L1前脂肪细胞增殖和分化的影响[J]. 中南医学科学杂志, 2013,41(1):7-9, 27.
Ling HY, Yang SS, Li X , et al. Effects of arecoline on the proliferation and differentiation of 3T3-L1 preadipocytes[J]. Med Sci J Central South China, 2013,41(1):7-9, 27.
[7] Harvey W, Scutt A, Meghji S , et al. Stimulation of human buccal mucosa fibroblasts in vitro by betel-nut alkaloids[J]. Arch Oral Biol, 1986,31(1):45-49.
[8] Chang YC, Tai KW, Lii CK , et al. Cytopathologic effects of arecoline on human gingival fibroblasts in vitro[J]. Clin Oral Investig, 1999,3(1):25-29.
[9] 王焕玲, 张建平 . 槟榔碱对人牙周膜成纤维细胞凋亡的影响[J]. 口腔疾病防治, 2017,25(4):223-226.
Wang HL, Zhang JP . Effects of arecoline on apoptosis of human periodontal ligament fibroblasts[J]. J Prev Treat Stomatol Dis, 2017,25(4):223-226.
[10] Jeng JH, Lan WH, Hahn LJ , et al. Inhibition of the migration, attachment, spreading, growth and collagen synjournal of human gingival fibroblasts by arecoline, a major areca alkaloid, in vitro[J]. J Oral Pathol Med, 1996,25(7):371-375.
[11] Khan I, Pant I, Narra S , et al. Epithelial atrophy in oral submucous fibrosis is mediated by copper (Ⅱ) and arecoline of areca nut[J]. J Cell Mol Med, 2015,19(10):2397-2412.
[12] Zhou ZS, Li M, Gao F , et al. Arecoline suppresses HaCaT cell proliferation through cell cycle regulatory molecules[J]. Oncol Rep, 2013,29(6):2438-2444.
[13] Cox S, Vickers ER, Ghu S , et al. Salivary arecoline levels during areca nut chewing in human volunteers[J]. J Oral Pathol Med, 2010,39(6):465-469.
[14] Sandulache VC, Parekh A, Li-Korotky H , et al. Prostaglandin E2 inhibition of keloid fibroblast migration, contraction, and transforming growth factor (TGF)-beta1-induced collagen synjournal[J]. Wound Repair Regen, 2007,15(1):122-133.
[15] Bugyi B, Carlier MF . Control of actin filament treadmilling in cell motility[J]. Annu Rev Biophys, 2010,39:449-470.
[16] Cheng HL, Su SJ, Huang LW , et al. Arecoline induces HA22T/VGH hepatoma cells to undergo anoikis - involvement of STAT3 and RhoA activation[J]. Mol Cancer, 2010,9:126.
[1] Zhou Jinkuo,Zhang Jinhong,Shi Xiaojing,Liu Guangshun,Jiang Lei,Liu Qianfeng. Influences of long noncoding RNA small nucleolar RNA host gene 22 on the cell proliferation, invasion and migration of oral squamous carcinoma cells by regulating microRNA-27b-3p [J]. Int J Stomatol, 2024, 51(1): 52-59.
[2] Xiong Menglin,Wu Long,Ma Li,Zhao Jin. Role of transforming growth factor-β2 in promoting the proliferation and differentiation of dental pulp stem cells [J]. Int J Stomatol, 2021, 48(6): 635-639.
[3] Liu Juan,Chen Bin,Yan Fuhua. Effects of platelet-rich plasma and concentrated growth factor on the proliferation and osteogenic differentiation of human periodontal cells [J]. Int J Stomatol, 2021, 48(5): 520-527.
[4] Zeng Kan,Li Xin,Wang Chenglin,Yang Jing,Ye Ling. Effects of bone microenvironment cells on tumor bone metastasis [J]. Int J Stomatol, 2020, 47(1): 95-101.
[5] Mei Hongxiang,Zhang Yidan,Zhang Chenghao,Liu Enyan,Chen Hao,Zhao Zhihe,Liao Wen. Effect of epigallocatechin-3-gallate on stem cell proliferation and osteogenic differentiation [J]. Int J Stomatol, 2019, 46(4): 431-436.
[6] Yuxuan Yang,Haixia Zhang,Shuang Wang. Biological function of amelogenin during periodontal regeneration [J]. Inter J Stomatol, 2019, 46(2): 191-196.
[7] Yuanyuan Li,Bin Cheng,Yun Wang. Effects of long non-coding RNA lnc-p26090 on the glycolysis and proliferation in oral squamous cell carcinoma [J]. Inter J Stomatol, 2018, 45(6): 628-634.
[8] Zhiqiang Wang,Yali Liu,Lijuan Ma,Lan Yang,Ruoyu Wang,Shuting Gao. Effects of salidroside on proliferation, apoptosis, cycle and migration of human tongue cancer CAL-27 cells [J]. Inter J Stomatol, 2018, 45(6): 678-685.
[9] Zhan Yeming, Zhang Mingzhu. Research progress on the relevance between drug-induced gingival overgrowth and cell proliferation and apoptosis [J]. Inter J Stomatol, 2018, 45(2): 199-203.
[10] Wang Qi, Chen Xiyan, Wen Yong.. Research progress on the crosstalk between Hippo/YAP signaling pathway and cell proliferation-related signaling pathways [J]. Inter J Stomatol, 2017, 44(5): 614-618.
[11] Tan Xiujun, Ye Ling, Wang Chenglin. Mechanisms of c-Jun N-terminal kinase signal transduction pathway regulating cell migration [J]. Inter J Stomatol, 2015, 42(5): 586-591.
[12] Li Ming, Peng Jieying, Wu Yingfang, Zhang Rui. Arecoline upregulated matrix metalloproteinase 9 expression in HaCaT cells [J]. Inter J Stomatol, 2015, 42(2): 166-169.
[13] Cheng Qun, Yang Minghua, Chen Bin, Liu Juan,Yan Fuhua. Effect of Er:YAG laser irradiation on the cell proliferation and migration of human periodontal ligament cells [J]. Inter J Stomatol, 2015, 42(2): 135-139.
[14] He Kun1, Cheng Xiangrong2, Zhang Ximu3. Influence of enamel matrix protein on attachment and proliferation ability of dental follicle cells and periodontal ligament cells cultured on titanium [J]. Inter J Stomatol, 2014, 41(5): 536-540.
[15] Huang Long, Jian Xinchun.. Areca nut-related carcinogens and oral cancer [J]. Inter J Stomatol, 2014, 41(1): 102-107.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[7] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[8] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[9] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[10] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .