Int J Stomatol

Int J Stomatol ›› 2020, Vol. 47 ›› Issue (5): 530-537.doi: 10.7518/gjkq.2020024

• Original Articles • Previous Articles     Next Articles

Influence of c57 mouse periodontitis model on cognitive ability

Tong Yuxin1,2,3(),Xiao Xinli4,An Ying3,Zhang Jiayu1,2,5,Shi Xuyan1,2,5,Wang Xu1,2,5,Chen Yue1,2,5()   

  1. 1.Key Laboratory of Shanxi Province for Craniofacial Precision Medicine Research, Xi’an 710032, China
    2.Clinical Research Center of Shanxi Province for Dental and Maxillofacial Diseases, Xi’an 710032, China
    3.State Key Laboratory of Millitary Stomatology & National Clinical Research Center for Oral Disease & Shanxi Engineering Research Center for Dental Materials and Advanced Manufacture & Dept. of Periodontology, Stomatology Hospital of Air Force Military Medical University, Xi’an 710032, China
    4.Medical School of Xi’an Jiaotong University, Xi’an 710061, China
    5.Dept. of Periodontology, Stomatological Hospital, Xi’an Jiaotong University, Xi’an 710004, China
  • Received:2019-12-20 Revised:2020-04-17 Online:2020-09-01 Published:2020-09-16
  • Contact: Yue Chen E-mail:wotyx101@163.com;dentistcy@126.com
  • Supported by:
    National Natural Science Foundation of China Youth Project(81700971);Natural Science Basic Research Program of Shanxi(2018JM7116)

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Abstract:

Objective This work aimed to establish a lipopolysaccharide (LPS)-induced animal model of periodontitis and observe the influence of LPS injection on the behaviour of c57 mice. Methods Twenty female c57 mice were ran-domly divided into two groups: blank control and experimental periodontitis groups. Mice in the experimental group were injected with Escherichia coli LPS by a microsyringe. Morris water maze (MWM) test was performed to assess the learning and memory ability of mice after 39 days. The expression levels of the proinflammatory cytokine interleukin (IL)-1β in mouse hippocampus tissues and serum were determined by enzyme-linked immunosorbent assay. Nissl body and neuron status in the CA3 area of the brain hippocampus were observed by Nissl staining, and alveolar bone resorption was evaluated by haematoxylin and eosin staining. Results In MWM positioning navigation experiment, the latency and thigmotaxis of both groups decreased gradually. The latency and thigmotaxis of the experimental group in the MWM experiment increased significantly compared with the control group (P<0.000 1). The number of neurons in the CA3 area of the experimental group mice increased significantly compared with the control group (P=0.001). The serum IL-1β content was higher in the experimental group than in the blank control group (P=0.007). Conclusion Long-term injection of low-dose LPS can induce bone loss and may cause cognitive impairment to c57 mice.

Key words: lipopolysaccharide, periodontitis, Alzheimer’s disease, cognitive impairment

CLC Number: 

  • R781.4+2

TrendMD: 

Fig 1

Results of Morris water maze latency in different groups of mice"

Fig 2

Results of Morris water maze thigmataxis in different groups of mice"

Fig 3

Nissl’s staining morphology of mice hippocampus"

Fig 4

Three dimensional reconstruction of MicroCT in periodontitis model of mice"

Fig 5

Observation of alveolar bone absorption with HE staining"

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