Int J Stomatol

Inter J Stomatol ›› 2019, Vol. 46 ›› Issue (2): 238-243.doi: 10.7518/gjkq.2019004

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Research progress on the extra- and intra-radicular biofilm associated with persistent apical periodontitis

Zi Yang,Benxiang Hou()   

  1. Dept. of Conservative Dentistry and Endodontics, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China
  • Received:2018-04-22 Revised:2018-10-15 Online:2019-03-01 Published:2019-03-15
  • Contact: Benxiang Hou E-mail:endohou@qq.com
  • Supported by:
    This study was supported by Beijing Municipal Administration of Hospital Clinical Medicine Development of Special Funding(XMLX201-30);Science and Technology Project of Dongcheng District, Beijing(2017-3-004)

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Abstract:

Persistent apical periodontitis is commonly related to the presence of intraradicular biofilm that persists in the complex apical root canal system and extraradicular biofilm. This review highlights the main differences and similarities between intraradicular and extraradicular biofilms, especially in structure and microbial composition. In addition, the relationship between intraradicular and extraradicular biofilms is summarized.

Key words: persistent apical periodontitis, extraradicular biofilm, intraradicular biofilm

CLC Number: 

  • R781.05

TrendMD: 

Tab 1

Bacterial respective genera and species commonly found in the root canal associated with PAP"

研究者 入组要求 样本数目/例 取样方法 检测方法 优势菌
Gomes等[16] 至少于4年前行根管治疗,仍有持续性根尖周低密度影 45 根管再治疗取根充物后无菌纸尖取样 PCR 粪肠球菌（77.8%）、消化链球菌（51.1%）、牙龈卟啉单胞菌（35.6%）、Filifactor alocis（26.7%）、齿垢密螺旋体（24.4%）、中间普氏菌（22.2%）、产黑普氏菌 （11.1%）、福赛斯坦纳菌（4.4%）
R??as等[14] 至少1年前行根管治疗,但仍存在根尖周低密度影 17 再治疗时取根充物 反向捕捉棋盘杂交法 链球菌属（47%）、乳杆菌属（35%）、难见戴阿利斯特杆菌（29%）,肠杆菌（29%）、中间普氏菌（29%）、生痰新月形单胞菌（29%）、互养菌属基因型BA121（29%）、梅毒螺旋体（29%）
Sakamoto等[15] 至少于5年前行根管治疗,患牙无临床症状,但仍有持续性根尖周低密度影 19 再治疗时取根充物 16S rRNA基因克隆文库 厚壁菌门、放线菌门、拟杆菌门、变形菌门、梭杆菌门、互养菌门
研究者 入组要求 样本数目/例 取样方法 检测方法 优势菌
Schirrmeister等[12] 至少于4年前行根管治疗,根充物距根尖不大于4 mm,根尖周有持续性低密度影 20 再治疗取根充后无菌纸尖取样 16S rRNA测序方法 具核梭杆菌、微小微单胞菌、难见戴阿利斯特杆菌、Solobacterium moorei、河流漫游球菌
Subramanian和Mi-ckel[17] 根管治疗后根充恰填但仍存在根尖周低密度影 34 根尖手术取根尖,去除外表面细菌后进行涡旋取样 16S rRNA测序方法 丙酸杆菌属、奈瑟氏菌属、月单胞菌属、营养缺陷菌属、双歧杆菌
Li等[18] 至少于2年前行根管治疗但仍存在根尖低密度影 7 再治疗取根充物后无菌纸尖取样 16S rRNA测序方法 洋葱伯克霍尔德氏菌、嗜二氧化碳噬细胞菌属、卟啉单胞菌属、茄科雷尔氏菌
Murad等[13] 至少于1年前行根管治疗但仍存在根尖低密度影 36 再治疗取根充物后无菌纸尖取样 棋盘DNA杂交 粪肠球菌、难见戴阿利斯特杆菌、表皮葡萄球菌、幽门螺旋杆菌
Antunes等[19] 至少于1年前行根管治疗,无症状但存在根尖低密度影 27 根尖手术取根尖外表面消毒后冷冻研磨法取样 实时PCR 链球菌属（76%）、放线菌门（52%）、假分支乳杆菌（19%）
Henriques等[20] 根管治疗后根尖周病变持续存在 40 再治疗取根充物后10#K锉取样 棋盘DNA杂交 白喉棒状杆菌、牙龈卟啉单胞菌,表兄链球菌、嗜麦芽糖寡养单胞菌

Tab 2

Bacterial respective genera and species commonly found in extraradicular biofilm associated with PAP"

研究者 入组要求 样本数目/例 取样方法 检测方法 优势菌
Noguchi等[21] 根管治疗后病变持续存在需行根尖手术者 27 根尖手术术中刮取牙根外表面生物膜 16S rRNA测序方法 具核梭杆菌、牙龈卟啉单胞菌、假丝酵母菌
Fujii等[22] 根管治疗后病变持续存在需行根尖手术者 20 根尖手术取根尖 16S rRNA测序方法 丙酸杆菌、表皮葡萄球菌、铜绿假单胞菌、具核梭杆菌
Wang等[8] 至少1年前行根管治疗,根尖病变直径<1 cm,需行根尖手术者 23 根尖手术取根尖 电子显微镜、改良Brown以及Brenn染色、PCR扩增及测序 放线菌属、丙酸杆菌属、普雷沃菌属、链球菌、牙龈卟啉单胞菌、伯克霍尔德氏菌
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[1] Li Hong, Hou Benxiang. Research progress on the extraradicular biofilm in persistent apical periodontitis [J]. Inter J Stomatol, 2013, 40(6): 754-757.
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