Int J Stomatol

Inter J Stomatol ›› 2018, Vol. 45 ›› Issue (6): 678-685.doi: 10.7518/gjkq.2018.06.010

• Original Articles • Previous Articles     Next Articles

Effects of salidroside on proliferation, apoptosis, cycle and migration of human tongue cancer CAL-27 cells

Zhiqiang Wang1,2,3,Yali Liu2,3,Lijuan Ma1,2,Lan Yang1(),Ruoyu Wang2,3,Shuting Gao1,3   

  1. 1. Dept. of Oral and Maxillofacial Surgery, The Second Hospital of Lanzhou University, Lanzhou 730030, China
    2. Key Laboratory of Stomatology of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China
    3. School of Stomatology, Lanzhou University, Lanzhou 730030, China
  • Received:2017-11-20 Revised:2018-07-22 Online:2018-11-01 Published:2018-11-15
  • Contact: Lan Yang E-mail:ylan2005@163.com
  • Supported by:
    This study was supported by Open Subject Funding Project of Key Laboratory of Stomatology, State Ethnic Affairs Commission (Northwest Minzu University)(MWZD201602)

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Abstract:

Objective To investigate the influence of salidroside on human tongue cancer cells (CAL-27) proliferation and migration.Methods 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT), flow cytometry instrument technique, Western blot and Transwell methods were used to detect the effect of salidroside for CAL-27 cell proliferation, apoptosis, cycle and migration.Results With increasing salidroside concentration and time, the influence on CAL-27 cell proliferation increased gradually, and the cell survival rate decreased obviously (P<0.05). With increasing salidroside concentration, the incidence of apoptosis of CAL-27 cell showed significant differences (P<0.05). Salidroside had a signifi-cant effect on the CAL-27 cell cycle and induced the period G0/G1 phase arrest of CAL-27 cell. With increasing salidroside concentration, CAL-27 cell migration was significantly reduced (P<0.05).Conclusion Salidroside affected proliferation, apoptosis, cycle and migration of human tongue cancer CAL-27 cells and had significant inhibitory effect in vitro.

Key words: salidroside, human tongue cancer CAL-27 cell, apoptosis, cell proliferation, cell cycle, cell migration

CLC Number: 

  • Q813.1 +1

TrendMD: 

Fig 1

Effects of different concentrations of salidroside on CAL27 cell inhibition in 24 and 48 h"

Fig 2

Effects of different concentrations of salidroside (48 h) on the cell of CAL-27 cell under Hoechst 33258 staining × 100"

Fig 3

Effects of different concentrations of salidroside on the growth of CAL-27 cell line (48 h) with Annexin V/PI staining were used to detect the apoptotic cells by flow cytometry"

Tab 1

Effects of different concentrations of salidroside on the growth of CAL-27 cell line (48 h) with flow cytometry were used to detect the apopto-tic cells cycle"

红景天苷浓度/（μg·mL-1） 不同细胞周期的细胞比例/%
G0/G1期 S期 G2/M期
20 45.03 28.22 0.83
40 62.99 25.37 0.12
60 72.76 21.32 0.16
80 76.96 15.50 0.06
100 81.28 14.82 0

Fig 4

Effects of different concentrations of salidroside on migration of CAL-27 cells (24 h) "

Fig 5

Effects of different concentrations of salidroside on protein expression of CAL-27 cells"

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