Int J Stomatol

Inter J Stomatol ›› 2015, Vol. 42 ›› Issue (6): 655-658.doi: 10.7518/gjkq.2015.06.010

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Recombination and expression of fimbriae protein for Porphyromonas gingivalis

Xiao Li1, Lin Yuxiang2, Ge Song3.   

  1. 1. Dept. of Periodontics, Hefei Stomatological Hospital, Hefei 230000, China; 2. Dept. of Periodontics, Xiamen Stomatological Hospital, Xiamen 361003, China; 3. Stomatological Hospital Affiliated to Zunyi Medical College, Zunyi 563003, China
  • Received:2015-03-10 Revised:2015-08-20 Online:2015-11-01 Published:2015-11-01

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Abstract:

Objective This study aimed to clone the type Ⅱ fimA gene of Porphyromonas gingivalis(P. gingivalis) and detect its expression in Escherichia coli(E. coli). Methods The type Ⅱ fimA gene was obtained by polymerase chain reaction(PCR) from the genome of P. gingivalis to construct a prokaryotic expression plasmid pET-FimA. pET-FimA was transformed into E. coli BL21(DE3) competent cells, and the recombination protein was characterized via sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and Western blot analysis. Results DNA sequencing showed that the fragment was 95% consistent with that of published literature. We successfully constructed prokaryotic expression plasmid pET-FimA. Conclusion The plasmid efficiently expressed the recombinant fimbriae protein of the type ⅡfimA gene of P. gingivalis. A high purity of protein FimA was obtained. FimA has immunogenicity and immune reactivity.

Key words: type Ⅱ fimA gene of Porphyromonas gingivalis, recombinant fimbriae protein, protein expression

CLC Number: 

  • R 782

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