Int J Stomatol

Inter J Stomatol ›› 2014, Vol. 41 ›› Issue (5): 541-545.doi: 10.7518/gjkq.2014.05.010

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The effect of arsenic trioxide on invasion of human tongue squamous carcinoma cell line CAL-27

Sun Xiangyu1,He Lina2, Lü Kewen1, Hu Tenglong1, Song Tao1, Jing Guangping1   

  1. 1. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China; 2. Dept. of Conservative Dentistry and Endodontics, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China
  • Received:2014-01-12 Revised:2014-05-26 Online:2014-09-01 Published:2014-09-01

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Abstract:

Objective To study the effect of arsenic trioxide on the invasion of human tongue squamous carcinoma cell line CAL-27 in vitro and in vivo and explore the underlying mechanisms. Methods Adhesion assay, Transwell chamber membrane invasion culture system, immunofluorescence and Western Blot were performed to detect the effect of arsenic trioxide on adhesion, migration and invasion of CAL-27 cells in vitro. Immunohistochemical and Western Blot assays were used to study the protein levels of CD44, matrix metalloproteinases(MMP)-2 and MMP-9 in vivo. Results In vitro, arsenic trioxide at lower-dose could significantly reduce abilities of CAL-27 cells to adhere, migrate and invade(P<0.05); the depolymerization of microfilaments was observed and fuzzy and discontinuous arrangements of microtubules were visualized upon arsenic trioxide treatment. In addition, the cells treated with lower-dose arsenic trioxide displayed reduced protein expressions of CD44, MMP-2 and MMP-9 in vivo. Conclusion Arsenic trioxide at lower-dose could inhibit the ability of the CAL-27 cell to invade by inhibiting cellular adhesion, modulating cytoskeleton and reducing the protein expressions of CD44, MMP-2 and MMP-9.

Key words: arsenic trioxide, human tongue squamous carcinoma cell line CAL-27, invasion

CLC Number: 

  • R 782

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