Inter J Stomatol ›› 2014, Vol. 41 ›› Issue (1): 26-30.doi: 10.7518/gjkq.2014.01.007

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Influence of proinflammatory cytokines in inducing mouse osteoclast-like cell formation in vitro

Yang Jun1, Ma Gang2, Zhang Ping1.   

  1. 1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 2. Dept. of Stomatology, The People’s Hospital of Shanshan in Xinjiang Uygur Autonomous Region, Shanshan 838200, China
  • Received:2013-07-20 Revised:2013-07-20 Online:2014-01-01 Published:2014-01-01

Abstract:

Objective This study aims to investigate the influence of proinflammatory cytokines interleukin(IL)-1, IL-6, and tumor necrosis factor(TNF)-α on differentiation and formation of mouse osteoclast-like cells in vitro. Methods Osteoclast precursor cells from mice spleen were cultured under four conditions. Group A was cultured with macrophage colony stimulating factor(M-CSF). Group B was cultured with M-CSF and the soluble ligand of receptor activator of NF- κB(sRANKL). Group C was cultured with M-CSF, sRANKL, IL-1, IL-6, and TNF-α. Finally, Group D was cultured with M-CSF, IL-1, IL-6, and TNF-α. All groups were cultured in 24-well plates with bone slices. Tartrate-resistant acid phosphatase staining and inverted microscope were employed to determine differentiation. A scanning electron microscope was used to examine the bone slices. Results The number of osteoclast-like cells from Groups C and B was significantly higher than that in Groups A and D(P<0.05). The number of cells in Group C was greater than that in Group B(P<0.05). No osteoclast-like cells were found in Groups A and D(P>0.05). Conclusion Proinflammatory cytokines IL-1, IL-6, and TNF-α can promote the differentiation of osteoclast-like cells in vitro, which is dependent on sRANKL. These cytokines cannot directly induce the formation of osteoclast-like cells.

Key words: osteoclast, cytokine, bone resorption, periodontitis


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