Int J Stomatol

Inter J Stomatol ›› 2013, Vol. 40 ›› Issue (3): 291-296.doi: 10.7518/gjkq.2013.03.004

Previous Articles     Next Articles

The effect of angiotensin Ⅱ on the expression of osteopontin and matrix metalloproteinase-2 in mice osteoblast

Li Guangyue, Xie Yun, Yue Yuan, Bai Congjia, Hao Liang, Wang Min   

  1. State Key Laboratory of Oral Diseases, Dept. of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2012-04-15 Revised:2012-11-30 Online:2013-05-01 Published:2013-05-01

RichHTML

2

PDF (PC)

546

Abstract:

Objective To analyze the effects of angiotensin（Ang）Ⅱ on the expression of osteopontin（OPN）and matrix metalloproteinase（MMP）-2 in osteoblast-like MC3T3-E1 cells and the type of receptors. Methods The mouse osteoblast -like MC3T3 -E1 cells were cultured, and divided into four groups, exposed to different concentrations of AngⅡ（10-8, 10-7, 10-6 mol•L-1） with one group as control. The secreted concentrations of OPN, MMP-2 were evaluated by enzyme-linked immunosorbent assay. In addition, the blockers of the two distinct types of receptors（AT1R and AT2R）were used to identify the receptor through which the Ang Ⅱ act in the osteoblastlike MC3T3-E1 cells. Results Ang Ⅱ, the concentration of which was within 10-8 to 10-6 mol•L-1 could increase the expressions of OPN, MMP -2 in a dose -dependent mannner. 10 -6 mol•L-1 Ang Ⅱ could stimulate the expressions of OPN, MMP-2 in a time-dependent mannner with the peak at the time of 24 h after the exposure. The AT1R blocker Losantan could significantly reduce the expressions of OPN and MMP-2 in the osteoblasts（P<0.05）, while the AT2R blocker PD123319 didn’t have this effect. Conclusion Ang Ⅱ could enhance the expressions of OPN and MMP-2 in dose- and time-dependent manners via AT1R.

Key words: osteoblast, angiotensin Ⅱ, osteopontin, matrix metalloproteinase-2


TrendMD: 
[1] Zhang Jingyi,Li Danwei,Sun Yu,Lei Yayan,Liu Tao,Gong Yu. In vitro cytotoxicity of composite resin and compomer and effect on osteogenic differentiation of osteoblasts [J]. Int J Stomatol, 2022, 49(4): 412-419.
[2] Lü Hui,Wang Hua,Sun Wen. T helper cell 17 and periodontitis related osteoimmunology [J]. Int J Stomatol, 2020, 47(6): 661-668.
[3] Sun Jianwei,Lei Lihong,Tan Jingyi,Chen Lili. Regulation of osteoimmunology by MicroRNA 155 and research progress of its possible mechanism in periodontitis [J]. Int J Stomatol, 2020, 47(5): 607-615.
[4] Ma Kai,Li Hao,Zhao Hongmei,Wang Yongliang,Liu Jie,Bai Na. Effects of inorganic bovine bone treated with low temperature argon-oxygen plasma on the adhesion, proliferation, and differentiation of MC3T3-E1 cells [J]. Int J Stomatol, 2020, 47(3): 278-285.
[5] Zhu Junjin,Zhou Jiaqi,Wu Yingying. Function of autophagy induced by mammalian target of rapamycin complex 1 in bone metabolism [J]. Int J Stomatol, 2020, 47(1): 84-89.
[6] Lu Kexin,Zhang Diya,Wu Yanmin. Research progress of protease-activated receptors on different types of cells in periodontal tissue [J]. Int J Stomatol, 2019, 46(6): 657-662.
[7] Fei Liu,Yuntao Zhang,Xiangrui Ma,Yajie Zhang,Yunhao Wang. Effect of the titanium surface immobilised by arginyl-glycyl-aspartate peptide self-assembling multilayers on mouse osteoblast-like cell MC3T3-E1 [J]. Inter J Stomatol, 2019, 46(2): 203-208.
[8] Xue Lingfa, Zhang Daizun, Xiao Wenlin, Yu Baojun. Mechanical strain induces mouse bone mesenchymal stem cells osteogenic differentiation [J]. Inter J Stomatol, 2017, 44(6): 679-685.
[9] Geng Yuanming1, Shen Xiaoqing1, Xu Pingping2.. Effects of biological stress and mitogen-activated protein kinase on bone remodeling [J]. Inter J Stomatol, 2016, 43(6): 700-705.
[10] Zhang Jiaxi1, He Meili1, Zhao Bingsong1, Li Linmei2. Roles of erythropoietin on the differentiation of osteoblasts [J]. Inter J Stomatol, 2016, 43(6): 636-639.
[11] Hou Yubo1, Liu Xinchan2, Yu Haiyan1, Cui Leihua3, Yu Weixian4. Effect of gingipains on osteoclasts and osteoblasts [J]. Inter J Stomatol, 2016, 43(5): 609-613.
[12] Huang Yihua, Ling Junqi. . Role of Toll-like receptor 2 and Toll-like receptor 4 in osteoblastic differentiation [J]. Inter J Stomatol, 2015, 42(4): 492-495.
[13] Zhou Zheng, Zhao Changming, Jiao Kai, Wang Meiqing. Regulatory role of sympathetic nervous system–adrenergic receptor on bone remodeling [J]. Inter J Stomatol, 2015, 42(3): 348-351.
[14] Zhang Jiaxi, Deng Hongyan, Ji Mingquan, Zhao Bingsong, Liu Dajun. Mechanism of action of erythropoietin and its receptor on osteoblasts [J]. Inter J Stomatol, 2015, 42(1): 102-105.
[15] Yan Ziqi1, He Wulin2, Zou Shujuan1.. Research progress on effect of low-level laser therapy during orthodontic tooth movement [J]. Inter J Stomatol, 2014, 41(2): 169-171.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[7] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[8] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[9] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[10] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .