Int J Stomatol

Inter J Stomatol ›› 2012, Vol. 39 ›› Issue (2): 168-171.doi: 10.3969/j.issn.1673-5749.2012.02.008

Previous Articles     Next Articles

Effects of sonicated extracts of Porphyromonas gingivalis on the viabilities of human periodontal ligament cells

Feng Qin, Zhang Fengqiu, Sun Zheng.   

  1. Dept. of Periodontics and Mucosa Disease, The Affiliated Beijing Stomatological Hospital of Capital Medical University, Beijing 100050, China
  • Received:2011-06-03 Revised:2011-12-20 Online:2012-03-01 Published:2012-03-01

RichHTML

2

PDF (PC)

1122

Abstract:

Objective To evaluate the effects of sonicated extracts of Porphyromonas gingivalis（P.gingivalis） on the viabilities of human periodontal ligament cells（hPDLC）. Methods In the experimental group, hPDLC obtained by the tissue culture were treated by sonicated extracts in different concentrations（6.25, 12.5, 25, 50 mg·L－1） for 24 hours. In the negative control group, hPDLC were treated without sonicated extracts. Furthermore, hPDLC were exposed to 12.5 mg·L－1 sonicated extracts for 24, 48, 72 and 96 h, while hPDLC in the negative group were cul - tured for 24, 48, 72 and 96 h without sonicated extracts. Cell viabilities in two groups were determined by methyl thiazolyl tetrazolium method and use SPSS 13.0 package for one-way analysis of variance and independent sample t-test. Results Sonicated extracts for hPDLC as the concentration of 6.25-50 mg·L－1, exposed for 24 h, the hPDLC viabilities of experimental group were lower than those of the negative group. When hPDLC were exposed to 12.5 mg·L－1 sonicated extracts, in the 24-96 h, the hPDLC viabilities of experimental group were lower than those of the control group. Conclusion Porphyromonas gingivalis can lead to decreased viabilities of hPDLC in vitro, in a dose and time dependent.

Key words: Porphyromonas gingivalis, extract, human periodontal ligament cell, viability


TrendMD: 
[1] Wang Renyi,Zhao Chengzhi,Pan Jian.. Research progress on the effects of prophylactic antibiotics on postoperative complications during the perioperative period of tooth extraction [J]. Int J Stomatol, 2023, 50(5): 558-565.
[2] Yin Maoyun,Zhang Yi,Hu Min.. Research progress on the effect of orthodontic extraction on upper airway [J]. Int J Stomatol, 2022, 49(5): 607-613.
[3] Wang Guanru,Feng Qiang.. Research progress on the role of Porphyromonas gingivalis on promoting the development of Alzheimer ’ s disease [J]. Int J Stomatol, 2022, 49(4): 397-403.
[4] Pang Yu,Liu Xian,Wang Liao. Application of digital template in the extraction of embedded supernumerary tooth [J]. Int J Stomatol, 2022, 49(4): 448-452.
[5] Liu Juan,Chen Bin,Yan Fuhua. Effects of platelet-rich plasma and concentrated growth factor on the proliferation and osteogenic differentiation of human periodontal cells [J]. Int J Stomatol, 2021, 48(5): 520-527.
[6] Hu Wenjie. Related problems in clinical practice of alveolar ridge preservation [J]. Int J Stomatol, 2021, 48(3): 249-259.
[7] Lei Shuang,Yu Jingjun,Tang Xiaolin. Research progress on the effects and mechanisms of Porphyromonas gingivalis on vascular endothelial cells from different tissues [J]. Int J Stomatol, 2021, 48(1): 23-28.
[8] Li Jingya,Shui Yusen,Guo Yongwen. Advances in mechanisms for osteogenic differentiation of human periodontal ligament cells induced by cyclic tensile stress [J]. Int J Stomatol, 2020, 47(6): 652-660.
[9] Chen Bin,Xu Rongrong,Zhang Jiading,Yan Fuhua. The retention of severe periodontitis-involved tooth [J]. Int J Stomatol, 2020, 47(2): 125-130.
[10] Zhang Mingshuang,Ba Te,Wang Wenbiao. Research progress on oral microorganisms affect Alzheimer’s disease [J]. Int J Stomatol, 2020, 47(1): 102-108.
[11] Zhang Zhiying,Liu Dongjuan,Pan Yaping. Research progress on Porphyromonas gingivalis outer membrane vesicles [J]. Int J Stomatol, 2019, 46(6): 670-674.
[12] Weilin Pan,Yubin Cao,Chang Liu,Jiyuan Liu,Chunjie Li,Jian Pan,Chengge Hua. Flap design associated pain after the extraction of mandibular third molars: a systematic review and Meta-analysis [J]. Inter J Stomatol, 2019, 46(2): 142-148.
[13] Tiancheng Li,Kai Xia,Shujuan Zou,Jun Liu. Effect of tooth extraction for the orthodontic treatment of the upper airway: a systematic review [J]. Inter J Stomatol, 2019, 46(2): 156-165.
[14] Yuhao Wang, Dianri Wang, Jian. Pan. Lingual nerve injury in the tooth extraction [J]. Inter J Stomatol, 2018, 45(5): 590-596.
[15] Ma Quanquan, Cai Xiaoxiao.. Current status of immediate implant placement and provisionalization in esthetics zone [J]. Inter J Stomatol, 2017, 44(6): 731-736.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[2] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[3] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[4] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[5] . [J]. Foreign Med Sci: Stomatol, 1999, 26(06): .
[6] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[7] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[8] . [J]. Foreign Med Sci: Stomatol, 1999, 26(05): .
[9] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .
[10] . [J]. Foreign Med Sci: Stomatol, 1999, 26(04): .