国际口腔医学杂志

国际口腔医学杂志 ›› 2022, Vol. 49 ›› Issue (3): 263-271.doi: 10.7518/gjkq.2022029

• 干细胞和再生医学专栏 • 上一篇    下一篇

非编码RNA调控牙囊干细胞成骨分化的研究进展

洪娅娅1(),陈学鹏1,姒蜜思2()   

  1. 1.浙江大学医学院附属口腔医院正畸科 浙江大学口腔医学院;浙江省口腔生物医学研究重点实验室 杭州 310006
    2.浙江大学医学院附属口腔医院种植科 浙江大学口腔医学院;浙江省口腔生物医学研究重点实验室 杭州 310006
  • 收稿日期:2021-09-29 修回日期:2021-12-09 出版日期:2022-05-01 发布日期:2022-05-09
  • 通讯作者: 姒蜜思
  • 作者简介:洪娅娅，住院医师，硕士，Email：yaya_zju@163.com
  • 基金资助:
    浙江省自然科学基金(LY18H140001)

Advances in research on noncoding RNA during the osteogenic differentiation of dental follicle stem cells

Hong Yaya1(),Chen Xuepeng1,Si Misi2()   

  1. 1.Dept. of Orthodontics, The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China
    2.Dept. of Implantology, The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China
  • Received:2021-09-29 Revised:2021-12-09 Online:2022-05-01 Published:2022-05-09
  • Contact: Misi Si
  • Supported by:
    Natural Science Foundation of Zhejiang Province(LY18H140001)

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摘要：

牙槽骨缺损的再生治疗一直是口腔领域亟待攻克的难点。牙周组织工程技术的出现为解决牙槽骨缺损提供了新思路，其中良好的种子细胞是实现组织再生的关键要素。牙囊干细胞（DFSC）具有成骨分化潜能，且易于获取和保存，是极具开发前景的种子细胞。DFSC成骨分化过程涉及复杂的基因调控，其中非编码RNA（ncRNA）作为一类从DNA转录而来但不编码蛋白质的功能性RNA，已被证实可在表观遗传水平、转录水平及转录后水平参与调控干细胞成骨分化。探索ncRNA在DFSC成骨分化调控网络中的角色和作用机制，可为组织再生提供新的治疗策略。本文就DFSC成骨分化过程中微小RNA（miRNA）、长链非编码RNA（lncRNA）和环状RNA（circRNA）等各类ncRNA调控作用的研究进展作一综述。

关键词: 牙囊干细胞, 非编码RNA, 微小RNA, 长链非编码RNA, 环状RNA, 成骨分化

Abstract:

Alveolar bone-defect regeneration has long been a challenge in the field of dentistry. The rising technology of periodontal tissue engineering provides a new strategy to solve it, and ideal seed cells are key elements of this technology. Dental follicle stem cells (DFSCs), as promising seed cells, have the potential of osteogenic differentiation, which are also easily accessible for cell culture and preservation. The osteogenic differentiation of DFSCs involves complex gene regulation. Noncoding RNA (ncRNA), as a functional RNA that is transcribed from DNA but does not encode protein, can reportedly regulate the osteogenic differentiation of stem cells at the epigenetic, transcription, and post-transcription le-vels. Exploring the role and mechanism of ncRNA in the regulatory network of DFSCs’ osteogenic differentiation can provide novel therapeutic strategies for tissue regeneration. This review summarizes the advances in research on ncRNA during the osteogenic differentiation of DFSCs, including microRNA, long noncoding RNA, and circular RNA.

Key words: dental follicle stem cell, noncoding RNA, micro RNA, long noncoding RNA, circular RNA, osteogenic differentiation

中图分类号: 

  • Q 254

表 1

参与调控DFSC成骨分化的ncRNA"

名称类型细胞调控水平表达水平调控作用可能的作用机制文献
miR-101miRNAhDFC转录后上调促进靶向BMP2/DLX3[51]
miR-29miRNAhDFC转录后下调抑制靶向COL1[52]
miR-146amiRNAhDFSC转录后下调抑制靶向EGFR[53]
miR-335miRNArDFSC转录后下调抑制—[54]
miR-214miRNArDFSC转录后下调抑制—[55]
miR-204miRNAhDFC转录后下调抑制靶向RUNX2、ALP、SPARC[56]
MEG3lncRNAhDFSC表观遗传下调抑制靶向Wnt信号通路[57]
HOTAIRM1lncRNAhDFSC表观遗传上调促进靶向DNMT1维持HOXA2的低甲基化水平[58]
circFgfr2circRNArDFC转录后上调促进充当miR-133的分子“海绵”，抑制miR-133表达，增强下游BMP6基因表达[59]
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