国际口腔医学杂志 ›› 2021, Vol. 48 ›› Issue (1): 50-57.doi: 10.7518/gjkq.2021013
Huang Junwen1,Qiao Jie2,Mei Zi1,Chen Zhuo1,Li Yang1,Qiao Bin1()
摘要:
目的 探讨脂多糖结合蛋白(LBP)在口腔鳞状细胞癌(OSCC)中的表达及其临床意义。方法 通过免疫组织化学实验分别检测LBP在48例OSCC患者的癌组织及其癌旁组织的表达情况,酶联免疫吸附试验分别检测130名OSCC患者和90名正常人血清中的LBP质量浓度,分析LBP与OSCC的临床病理特点的关系,并绘制受试者工作特征(ROC)曲线分析血清中LBP质量浓度在OSCC诊断中的价值。结果 与癌旁组织相比,LBP在OSCC组织中高表达(P<0.001),并且LBP与OSCC的肿瘤分期、淋巴结转移及临床分期有关,未发现LBP与OSCC患者的性别、年龄及病理分级有关(P>0.1)。同时,通过免疫组织化学实验发现CD204在OSCC中有类似的结果。与正常人相比,OSCC患者血清中LBP质量浓度升高(P<0.001),并与OSCC的肿瘤分期和临床分期有关(P<0.001)。ROC曲线下面积为0.876(P<0.001),LBP血清质量浓度的最佳截断值为0.733 μg·mL -1,特异性93.3%,敏感性80.0%。 结论 LBP与OSCC的发生发展有关,可能是其潜在的肿瘤标志物,提示LBP在OSCC的诊断中具有潜在的临床价值。
中图分类号:
[1] | Bray F, Ferlay J, Soerjomataram I , et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018,68(6):394-424. |
[2] | Siegel RL, Miller KD, Jemal A . Cancer statistics, 2019[J]. CA A Cancer J Clin, 2019,69(1):7-34. |
[3] | Grivennikov SI, Greten FR, Karin M . Immunity, inflammation, and cancer[J]. Cell, 2010,140(6):883-899. |
[4] |
Hanahan D, Weinberg RA . Hallmarks of cancer: the next generation[J]. Cell, 2011,144(5):646-674.
doi: 10.1016/j.cell.2011.02.013 pmid: 21376230 |
[5] | Schumann RR, Leong SR, Flaggs GW , et al. Structure and function of lipopolysaccharide binding protein[J]. Science, 1990,249(4975):1429-1431. |
[6] |
Zweigner J, Schumann RR, Weber JR . The role of lipopolysaccharide-binding protein in modulating the innate immune response[J]. Microbes Infect, 2006,8(3):946-952.
doi: 10.1016/j.micinf.2005.10.006 pmid: 16483818 |
[7] | Tsukamoto H, Takeuchi S, Kubota K , et al. Lipopolysaccharide (LPS)-binding protein stimulates CD 14-dependent Toll-like receptor 4 internalization and LPS-induced TBK1-IKKϵ-IRF3 Axis activation[J]. J Biol Chem, 2018,293(26):10186-10201. |
[8] | Ryu JK, Kim SJ, Rah SH , et al. Reconstruction of LPS transfer cascade reveals structural determinants within LBP, CD14, and TLR4-MD2 for efficient LPS recognition and transfer[J]. Immunity, 2017,46(1):38-50. |
[9] | Kopp F, Kupsch S, Schromm AB . Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: implications for a role of LBP in intracellular LPS-signaling[J]. Biochim Biophys Acta, 2016,1863(4):660-672. |
[10] | Boylan KL, Andersen JD, Anderson LB , et al. Quantitative proteomic analysis by iTRAQ(R) for the iden-tification of candidate biomarkers in ovarian cancer serum[J]. Proteome Sci, 2010,8:31. |
[11] | Kovacs G, Peterfi L, Farkas N , et al. Expression of inflammatory lipopolysaccharide binding protein (LBP) predicts the progression of conventional renal cell carcinoma‒a short report[J]. Cell Oncol (Dordr), 2017,40(6):651-656. |
[12] | Peterfi L, Yusenko MV, Kovacs G . IL6 shapes an inflammatory microenvironment and triggers the development of unique types of cancer in end-stage kidney[J]. Anticancer Res, 2019,39(4):1869-1874. |
[13] | Wang N, Song XG, Liu LS , et al. Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer[J]. Cancer Sci, 2018,109(5):1701-1709. |
[14] | Kouketsu A, Sato I, Oikawa M , et al. Regulatory T cells and M2-polarized tumour-associated macropha-ges are associated with the oncogenesis and progression of oral squamous cell carcinoma[J]. Int J Oral Maxillofac Surg, 2019,48(10):1279-1288. |
[15] | Yamagata Y, Tomioka H, Sakamoto K , et al. CD163-positive macrophages within the tumor stroma are associated with lymphangiogenesis and lymph node metastasis in oral squamous cell carcinoma[J]. J O-ral Maxillofac Surg, 2017,75(10):2144-2153. |
[16] | ASMRHaque, Moriyama M, Kubota K, et al. CD206 + tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production [J]. Sci Rep, 2019,9(1):14611. |
[17] | Wang B, Liu H, Dong XL , et al. High CD204 + tumor-infiltrating macrophage density predicts a poor prognosis in patients with urothelial cell carcinoma of the bladder [J]. Oncotarget, 2015,6(24):20204-20214. |
[18] | Kawachi A, Yoshida H, Kitano S , et al. Tumor-associated CD204 + M2 macrophages are unfavorable pro-gnostic indicators in uterine cervical adenocarcinoma [J]. Cancer Sci, 2018,109(3):863-870. |
[19] | Wang YP, Chen C, Hua QQ , et al. Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma[J]. Oncol Rep, 2017,37(3):1503-1510. |
[20] | Chen R, Luo FK, Wang YL , et al. LBP and CD14 polymorphisms correlate with increased colorectal carcinoma risk in Han Chinese[J]. World J Gastroenterol, 2011,17(18):2326-2331. |
[21] | Chang CM, Chia VM, Gunter MJ , et al. Innate immunity gene polymorphisms and the risk of colorectal neoplasia[J]. Carcinogenesis, 2013,34(11):2512-2520. |
[22] | Cai QY, Jiang JH, Jin RM , et al. The clinical significance of lipopolysaccharide binding protein in hepatocellular carcinoma[J]. Oncol Lett, 2020,19(1):159-166. |
[23] | Kubota K, Moriyama M, Furukawa S , et al. CD163 + CD204 + tumor-associated macrophages contribute to T cell regulation via interleukin-10 and PD-L1 production in oral squamous cell carcinoma [J]. Sci Rep, 2017,7(1):1755. |
[24] | Kurago ZB, Lam-ubol A, Stetsenko A, et al. Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation[J].Head Neck Pathol, 2008,2(1):1-12. |
[25] | Utispan K, Pugdee K, Koontongkaew S . Porphyromonas gingivalis lipopolysaccharide-induced ma-crophages modulate proliferation and invasion of head and neck cancer cell lines[J]. Biomed Pharmacother, 2018,101:988-995. |
[1] | 周金阔,张晋弘,史晓晶,刘广顺,姜磊,刘倩峰. 长链非编码RNA小核仁RNA宿主基因22调控微小RNA-27b-3p对口腔鳞状细胞癌细胞增殖、侵袭和迁移的影响[J]. 国际口腔医学杂志, 2024, 51(1): 52-59. |
[2] | 李立恒,王蕊,王晓明,张智轶,张璇,安峰,王芹,张凡. 环状RNA hsa_circ_0085576调控微小RNA-498/B细胞特异性莫洛尼鼠白血病病毒整合位点1轴对口腔鳞状细胞癌细胞迁移和侵袭的影响[J]. 国际口腔医学杂志, 2024, 51(1): 60-67. |
[3] | 吴佳敏,夏斌,杨禾丰,许彪. 癌相关成纤维细胞在口腔鳞状细胞癌微环境中作用的研究进展[J]. 国际口腔医学杂志, 2023, 50(6): 711-717. |
[4] | 柳江龙, 买买提吐逊·吐尔地. 超声造影在口腔鳞状细胞癌颈部转移性淋巴结诊断中的研究进展[J]. 国际口腔医学杂志, 2023, 50(5): 514-520. |
[5] | 盛南宁,王珏,南欣荣. 性别决定基因盒9在口腔鳞状细胞癌作用机制和治疗中的研究进展[J]. 国际口腔医学杂志, 2023, 50(3): 314-320. |
[6] | 李潭,梁新华. 盘状蛋白结构域受体1在调控恶性肿瘤进展和治疗中的作用[J]. 国际口腔医学杂志, 2023, 50(2): 230-236. |
[7] | 赵卓平,辛鹏飞,高阳,张彩凤,张宽收,刘青梅. 光热治疗在口腔鳞状细胞癌治疗中的研究进展[J]. 国际口腔医学杂志, 2022, 49(4): 462-470. |
[8] | 江涵,神应强,陈谦明. 毒蕈碱受体通过Yes相关蛋白信号对口腔鳞状细胞癌生物学行为的实验研究[J]. 国际口腔医学杂志, 2022, 49(2): 138-143. |
[9] | 蒋宇磊,夏斌,饶南荃,杨禾丰,许彪. 外泌体在口腔鳞状细胞癌恶性进展及诊疗应用的研究[J]. 国际口腔医学杂志, 2021, 48(6): 711-717. |
[10] | 甘建国,高攀,王晓毅. 循环肿瘤细胞与口腔鳞状细胞癌相关性的研究进展[J]. 国际口腔医学杂志, 2021, 48(2): 205-212. |
[11] | 何宇晴,但红霞,陈谦明. 光动力疗法在口腔黏膜癌变防治中的应用[J]. 国际口腔医学杂志, 2020, 47(6): 669-676. |
[12] | 郝福,宁毅,孙睿,郑晓旭. 口腔鳞状细胞癌中转化因子2β的表达及潜在的临床意义[J]. 国际口腔医学杂志, 2020, 47(2): 159-165. |
[13] | 薛伶俐,李雅冬. 经首次根治性手术治疗口腔鳞状细胞癌患者的生存相关影响因素分析[J]. 国际口腔医学杂志, 2020, 47(2): 166-174. |
[14] | 董云梅,陶艳,周瑜. 口腔黏膜癌变过程中血清生化标志物的研究进展[J]. 国际口腔医学杂志, 2020, 47(1): 43-50. |
[15] | 陈宏丽,杨敬,尹刚,李皓缘,乔燕. 锌指蛋白32在口腔鳞状细胞癌中的表达意义及对口腔鳞状细胞癌干细胞的影响[J]. 国际口腔医学杂志, 2019, 46(6): 631-639. |
|