Abstract: Objective This study investigated the in vitro antimicrobial ability of pure titanium when pre-soaked with organosilicon quaternary ammonium salt. Methods The pure titanium specimens were randomly divided into six groups, five of which were soaked with different concentrations of organic silicon quaternary ammonium salt solution at 1%, 5%, 10%, 30%, and 60%, while one was treated with distilled water as a control. Each group was further divided into four different treatment times(30, 60, 120, and 240 min). The turbidimetric method was used to analyze the effect of organic silicon to the growth of Candida albicans on the pure titanium. The experimental groups were subjected to 0, 2 500, 5 000, and 10 000 cycles of hot and cold experiments and exposure to bacterial culture. The long-term antimicrobial properties of the treated titanium was calculated through a hot and cold cycle experiment. Results Different concentrations significantly affect the growth ofCandida albicans, but the processing time does not. There is an interaction between concentration and processing time. Titanium soaked with 1% organosilicon quaternary ammonium salt is potentially antibacterial, and its antibacterial effect reaches a maximum upon 120 min soaking. After 5 000 cycles hot and cold experiments, the pure titanium retained its antibacterial property; however, the antibacterial activity was lost after 10 000 cycles. Conclusion After soaking with the organosilicon quaternary ammo-nium salt solution, the pure titanium can maintain its long-acting antibacterial properties.

Key words: organosilicon quaternary ammonium salt, pure titanium, Candida albicans, antibacterial properties