国际口腔医学杂志

国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (3): 255-260.doi: 10.7518/gjkq.2018.03.002

• 干细胞专栏 • 上一篇    下一篇

体外培养环境影响牙周膜干细胞生物学特性的研究进展

贾凌璐1,2, 文勇1,2, 徐欣1,2   

  1. 1.山东大学口腔医学院 济南 250012;
    2.山东省口腔组织再生重点实验室 济南 250012
  • 收稿日期:2017-09-28 修回日期:2018-01-15 出版日期:2018-05-01 发布日期:2018-05-01
  • 通讯作者: 文勇,副教授,博士,Email：wenyong@sdu.edu.cn
  • 作者简介:贾凌璐,博士,Email：danlingjia@163.com
  • 基金资助:
    山东省重点研发计划（2016GSF201115,2017GSF181- 17）;中国博士后基金 （2017M610432）;山东省医药卫生发展计划（2017WS111）

Effects of culture conditions in vitro on the biological characteristics of periodontal ligament stem cells

Jia Linglu1,2, Wen Yong1,2, Xu Xin1,2   

  1. 1. School of Stomatology, Shandong University, Jinan 250012, China;
    2. Shandong Province Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China
  • Received:2017-09-28 Revised:2018-01-15 Online:2018-05-01 Published:2018-05-01
  • Supported by:
    This study was supported by Shandong Provincial Key Research and Development Program (2016GSF201115, 2017GSF18117), China Postdoctoral Science Foundation (2017M610432) and Shandong Pharmaceutical and Health Science Technology Development Programme (2017WS111).

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摘要： 牙周膜干细胞是具有自我更新能力与多向分化潜能的成体干细胞,作为口腔组织工程研究的候选种子细胞备受关注。进行体外培养过程中,原代细胞分离方法、基础培养基成分、氧气浓度、培养时间、细胞保存条件及其他物理和化学刺激的施加等培养因素可在一定程度上改变牙周膜干细胞的增殖、分化、迁移等特性,将影响其在组织工程中应用的效果。本文围绕体外培养环境对牙周膜干细胞生物学特性的影响进行综述,为优化牙周膜干细胞体外培养条件、充分发挥其全能性提供参考。

关键词: 牙周膜干细胞, 细胞培养技术, 生物学特性

Abstract: Periodontal ligament stem cell, which is a kind of adult stem cell, owns the abilities of self-renewing and multi-differentiation and has become the hot spot in the oral tissue engineering. When cultured in vitro, different culture conditions such as primary cell separation methods, culture medium composition, oxygen concentration, culture time, cell storage conditions and other physical or chemical stimulation can change the proliferation, immigration or differentiation characteristics of periodontal ligament stem cell. This paper is committed to review on the research progress regarding the effects of the culture conditions in vitro on the biological characteristics of periodontal ligament stem cell in recent years and provide theoretical reference for tissue engineering.

Key words: periodontal ligament stem cell, cell culture technique, biological characteristics

中图分类号: 

  • Q813.1+1
[1] Seo BM, Miura M, Gronthos S, et al.Investigation of multipotent postnatal stem cells from human periodontal ligament[J]. Lancet, 2004, 364(9429): 149-155.
[2] Wang L, Shen H, Zheng W, et al.Characterization of stem cells from alveolar periodontal ligament[J]. Tissue Eng Part A, 2011, 17(7/8):1015-1026.
[3] Huang GTJ, Gronthos S, Shi S.Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medi-cine[J]. J Dent Res, 2009, 88(9):792-806.
[4] Bueno C, Ramirez C, Rodríguez-Lozano FJ, et al.Human adult periodontal ligament-derived cells integrate and differentiate after implantation into the adult mammalian brain[J]. Cell Transplant, 2013, 22(11):2017-2028.
[5] 刘娟, 孟波, 赵华, 等. 人牙周膜细胞不同分离培养方法的比较研究[J]. 广东牙病防治, 2011, 19(12): 631-634.
Liu J, Meng B, Zhao H, et al.Methods for isolation and culture of human periodontal ligament cell: a comparative study[J]. J Dent Prev Treat, 2011, 19(12):631-634.
[6] Prateeptongkum E, Klingelhöffer C, Müller S, et al.Characterization of progenitor cells and stem cells from the periodontal ligament tissue derived from a single person[J]. J Periodont Res, 2016, 51(2):265-272.
[7] Tanaka K, Iwasaki K, Feghali KE, et al.Comparison of characteristics of periodontal ligament cells ob-tained from outgrowth and enzyme-digested culture methods[J]. Arch Oral Biol, 2011, 56(4):380-388.
[8] Jung IH, Kwon BS, Kim SH, et al.Optimal medium formulation for the long-term expansion and main-tenance of human periodontal ligament stem cells[J]. J Periodontol, 2013, 84(10):1434-1444.
[9] 鄂玲玲, 刘弘臣, 王懿, 等. 血清浓度及培养时间对牙周膜细胞生物学活性的影响[J]. 上海口腔医学, 2010, 19(3):275-280.
E LL, Liu HC, Wang Y, et al. The effect of serum concentration and culture time on the biologic activi-ty of human periodontal ligament cells[J]. Shanghai J Stomatol, 2010, 19(3):275-280.
[10] Hou LT, Li TI, Liu CM, et al.Modulation of osteo-genic potential by recombinant human bone morpho-genic protein-2 in human periodontal ligament cells: effect of serum, culture medium, and osteoinductive medium[J]. J Periodont Res, 2007, 42(3):244-252.
[11] Wu RX, Yu Y, Yin Y, et al.Platelet lysate supports the in vitro expansion of human periodontal ligament stem cells for cytotherapeutic use[J]. J Tissue Eng Regen Med, 2017, 11(8):2261-2275.
[12] Tarle SA, Shi S, Kaigler D.Development of a serum-free system to expand dental-derived stem cells: PDLSCs and SHEDs[J]. J Cell Physiol, 2011, 226(1):66-73.
[13] 邹菡, 文韵笙, 吴志玲, 等. 低氧对人牙周膜细胞增殖、细胞周期及分化潜能的影响[J/OL]. 中华口腔医学研究杂志(电子版), 2015, 9(3):177-184.
Zou H, Wen YS, Wu ZL, et al.A preliminary study on effects of hypoxia on the proliferation, cell cycle and differentiation of human periodontal ligament cells[J/OL]. Chin J Stomatol Res (Elect Ed), 2015, 9(3): 177-184.
[14] Zhou Y, Fan W, Xiao Y.The effect of hypoxia on the stemness and differentiation capacity of PDLC and DPC[J]. Biomed Res Int, 2014, 2014:890675.
[15] Kawasaki T, Sumita Y, Egashira K, et al.Transient exposure to hypoxic and anoxic oxygen concentra-tions promotes either osteogenic or ligamentogenic characteristics of PDL cells[J]. Biores Open Access, 2015, 4(1):175-187.
[16] Gölz L, Memmert S, Rath-Deschner B, et al.Hypoxia and P. gingivalis synergistically induce HIF-1 and NF-κB activation in PDL cells and periodontal diseases[J]. Mediators Inflamm, 2015, 2015:438085.
[17] Choi JK, Hwang HI, Jang YJ.The efficiency of the in vitro osteo/dentinogenic differentiation of human dental pulp cells, periodontal ligament cells and gin-gival fibroblasts[J]. Int J Mol Med, 2015, 35(1):161-168.
[18] Jayaraman P, Govindasamy V, Gnanasegaran N, et al.Expression patterns of immune genes in long-term cultured dental stem cells[J]. Clin Oral Investig, 2016, 20(1):109-116.
[19] 徐婕, 刘宏伟. 低温冻存对人牙周膜干细胞生物学特性的影响[J]. 中国医药指南, 2009, 7(17):20-21.
Xu J, Liu HW.Effects of cryopreservation on biolo-gical characters of human PDLSCs[J]. Guide Chin Med, 2009, 7(17):20-21.
[20] 魏福兰, 马晓妮, 张凡. 深低温冻存对牙周膜干细胞膜片增殖和分化能力的影响[J]. 口腔生物医学, 2015, 6(2):87-89.
Wei FL, Ma XN, Zhang F.Effect of cryopreserva-tion on proliferation and differentiation of periodon-tal ligament stem cell sheet[J]. Oral Biomed, 2015, 6(2):87-89.
[21] Rhim EM, Ahn SJ, Kim JY, et al.Cryopreservation induces macrophage colony stimulating factor from human periodontal ligament cells in vitro[J]. Cryo-biology, 2013, 67(2):156-162.
[22] 王漩, 刘奕杉, 马艳, 等. 不同组织冻存体系对牙周膜干细胞体外扩增的影响[J]. 中国组织工程研究, 2013, 17(32):5855-5862.
Wang X, Liu YB, Ma Y, et al.Periodontal ligament stem cells expansion in vitro under different cryopre-servation systems[J]. J Clin Rehab Tissue Eng Res, 2013, 17(32):5855-5862.
[23] Lee SY, Huang GW, Shiung JN, et al.Magnetic cryo-preservation for dental pulp stem cells[J]. Cells Tissues Organs, 2012, 196(1):23-33.
[24] Zhang C, Li J, Zhang L, et al.Effects of mechanical vibration on proliferation and osteogenic differentia-tion of human periodontal ligament stem cells[J]. Arch Oral Biol, 2012, 57(10):1395-1407.
[25] Zhang C, Lu Y, Zhang L, et al.Influence of different intensities of vibration on proliferation and differen-tiation of human periodontal ligament stem cells[J]. Arch Med Sci, 2015, 11(3):638-646.
[26] Shen T, Qiu L, Chang H, et al.Cyclic tension promo-tes osteogenic differentiation in human periodontal ligament stem cells[J]. Int J Clin Exp Pathol, 2014, 7(11):7872-7880.
[27] Yang SY, Wei FL, Hu LH, et al.PERK-eIF2α-ATF4 pathway mediated by endoplasmic reticulum stress response is involved in osteodifferentiation of human periodontal ligament cells under cyclic mechanical force[J]. Cell Signal, 2016, 28(8):880-886.
[28] Suzuki R, Nemoto E, Shimauchi H.Cyclic tensile force up-regulates BMP-2 expression through MAP kinase and COX-2/PGE2 signaling pathways in hu-man periodontal ligament cells[J]. Exp Cell Res, 2014, 323(1):232-241.
[29] Gao Q, Walmsley AD, Cooper PR, et al.Ultrasound stimulation of different dental stem cell populations: role of mitogen activated protein kinase signaling[J]. J Endod, 2016, 42(3):425-431.
[30] Soares DM, Ginani F, Henriques ÁG, et al.Effects of laser therapy on the proliferation of human perio-dontal ligament stem cells[J]. Lasers Med Sci, 2015, 30(3):1171-1174.
[31] Cmielova J, Havelek R, Soukup T, et al.Gamma radiation induces senescence in human adult mesen-chymal stem cells from bone marrow and periodontal ligaments[J]. Int J Radiat Biol, 2012, 88(5):393-404.
[32] Kono K, Maeda H, Fujii S, et al.Exposure to trans-forming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines[J]. Cell Tissue Res, 2013, 352(2):249-263.
[33] Yuda A, Maeda H, Fujii S, et al.Effect of CTGF/CCN2 on osteo/cementoblastic and fibroblastic dif-ferentiation of a human periodontal ligament stem/progenitor cell line[J]. J Cell Physiol, 2015, 230(1): 150-159.
[34] Zhu W, Tan Y, Qiu Q, et al.Comparison of the pro-perties of human CD146+ and CD146- periodontal ligament cells in response to stimulation with tumour necrosis factor α[J]. Arch Oral Biol, 2013, 58(12): 1791-1803.
[35] Choi S, Cho TJ, Kwon SK, et al.Chondrogenesis of periodontal ligament stem cells by transforming growthfactor-β3 and bone morphogenetic protein-6 in a normal healthy impacted third molar[J]. Int J Oral Sci, 2013, 5(1):7-13.
[36] Yu Y, Mu J, Fan Z, et al.Insulin-like growth factor 1 enhances the proliferation and osteogenic differen-tiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways[J]. Histochem Cell Biol, 2012, 137(4):513-525.
[37] Choi SS, Park EK, Kwack MH, et al.Effects of de-xamethasone, a synthetic glucocorticoid, on human periodontal ligament stem cells[J]. Naunyn Schmie-debergs Arch Pharmacol, 2015, 388(9):991-995.
[38] Jin Z, Feng Y, Liu H.Conditioned media from dif-ferentiating craniofacial bone marrow stromal cells influence mineralization and proliferation in perio-dontal ligament stem cells[J]. Hum Cell, 2016, 29(4):162-175.
[39] Zhao L, Wu Y, Tan L, et al.Coculture with endothe-lial cells enhances osteogenic differentiation of per-dontal ligament stem cells via cyclooxygenase-2/prostaglandin E2/vascular endothelial growth factor signaling under hypoxia[J]. J Periodontol, 2013, 84(12):1847-1857.
[40] Vera-Sánchez M, Aznar-Cervantes S, Jover E, et al.Silk-fibroin and graphene oxide composites promote human periodontal ligament stem cell spontaneous differentiation into osteo/cementoblast-like cells[J]. Stem Cells Dev, 2016, 25(22):1742-1754.
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