国际口腔医学杂志 ›› 2017, Vol. 44 ›› Issue (2): 179-182.doi: 10.7518/gjkq.2017.02.013
崔军,崔光辉,贾黎,金龙,方珍
Cui Jun, Cui Guanghui, Jia Li, Jin Long, Fang Zhen.
摘要: 目的探讨流体剪切力(FSS)对人脂肪基质细胞(ADSC)成骨相关基因骨钙素(OCN)、转录因子Osterix(Osx)及核心结合因子(Cbf)a1 / Runt相关转录因子(Runx)2表达的影响。方法将脂肪抽吸术获取的人体脂肪进行常规培养(A1、A2组)和诱导培养(A3、A4组),然后分别对其加载强度为0.3 Pa的FSS(A1、A3组),未加FSS的为对照组(A2、A4组),通过逆转录-聚合酶链反应检测骨钙素、转录因子Osx及Cbfa1/Runx2基因表达的情况。结果A3、A4组在FSS加载后,其成骨相关基因的表达增强;而A1、A2组未见成骨特异性基因条带。这表明在常规培养条件下,无论是否有FSS的刺激,均无成骨特异性基因表达。结论脂肪基质细胞在诱导培养条件下, FSS可促使其向成骨细胞分化,可作为骨组织工程的种子细胞。
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