国际口腔医学杂志 ›› 2022, Vol. 49 ›› Issue (6): 684-689.doi: 10.7518/gjkq.2022092

• 牙髓治疗专栏 • 上一篇    下一篇

浓缩生长因子在活髓保存治疗中的应用前景

周灿(),曾倩,韦曦()   

  1. 中山大学附属口腔医院牙体牙髓病科 中山大学光华口腔医学院广东省口腔医学重点实验室 广州 510055
  • 收稿日期:2022-01-15 修回日期:2022-05-18 出版日期:2022-11-01 发布日期:2022-11-03
  • 通讯作者: 韦曦
  • 作者简介:周灿,硕士,Email:zhouc6@mail2.sysu.edu.cn
  • 基金资助:
    广东省财政高水平医院建设专项资金(174-2018-XMZC-0001-03-0125/D-08)

Prospects for the application of concentrated growth factor in vital pulp therapy

Zhou Can(),Zeng Qian,Wei Xi.()   

  1. Dept. of Cariology and Endodontics, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
  • Received:2022-01-15 Revised:2022-05-18 Online:2022-11-01 Published:2022-11-03
  • Contact: Xi. Wei
  • Supported by:
    Guangdong Financial Fund for High-Caliber Hospital Construction(174-2018-XMZC-0001-03-0125/D-08)

摘要:

活髓保存治疗是牙髓损伤的微创治疗手段,盖髓材料的不断优化对其疗效起重要作用。研究表明,生物陶瓷类材料负载生物活性小分子物质进行活髓保存治疗可诱导牙髓牙本质复合体生成。浓缩生长因子(CGF)属第3代自体血小板浓缩物,富含白细胞及大量生物活性因子,在活髓保存治疗中存在广阔的应用前景。本文对CGF的发展历程、制备方法及活性成分进行归纳,并将其应用于组织修复再生的相关研究进行综述,为CGF在活髓保存治疗的应用提供思路和研究方向。

关键词: 血液浓缩物, 浓缩生长因子, 牙髓损伤, 活髓保存治疗

Abstract:

Vital pulp therapy (VPT) is a minimally invasive treatment for pulp injury, and the continuous optimization of pulp capping materials plays an important role in its curative effect. Studies have shown that bio ceramic materials loaded with biologically active small molecules for VPT can induce dental pulp-dentin complexes. Concentrated growth factor (CGF) is a third-generation autologous platelet concentrate, rich in white blood cells and a large number of biologically active factors, and has broad application prospects in VPT. This article summarizes the development, preparation methods, and active ingredients of CGF, and reviews the related research on its application in tissue repair and regeneration, which can provide new ideas for the research of CGF in VPT.

Key words: autologous blood concentrates, concentrated growth factor, pulp injury, vital pulp therapy

中图分类号: 

  • R 781
1 da Rosa WLO, Cocco AR, Silva TMD, et al. Current trends and future perspectives of dental pulp capping materials: a systematic review[J]. J Biomed Mater Res B Appl Biomater, 2018, 106(3): 1358-1368.
2 吴补领, 罗奕菲, 徐稳安, 等. 恒牙牙髓炎的活髓保存治疗[J]. 口腔疾病防治, 2021(7): 433-441.
Wu BL, Luo YF, Xu WA, et al. Vital pulp therapy of pulp inflammation of permanent teeth[J]. J Prev Treat Stomatol Dis, 2021(7): 433-441.
3 Gandolfi MG, Spagnuolo G, Siboni F, et al. Calcium silicate/calcium phosphate biphasic cements for vital pulp therapy: chemical-physical properties and human pulp cells response[J]. Clin Oral Investig, 2015, 19(8): 2075-2089.
4 周学东, 黄定明, 刘建国, 等. 牙髓损伤的活髓保存治疗[J]. 华西口腔医学杂志, 2017, 35(4): 339-347.
Zhou XD, Huang DM, Liu JG, et al. Vital pulp therapy of damaged dental pulp[J]. West China J Stomatol, 2017, 35(4): 339-347.
5 杨蕊琦, 韦曦. 恒牙活髓保存治疗新进展[J]. 牙体牙髓牙周病学杂志, 2017, 27(7): 410-417.
Yang RQ, Wei X. New approaches of vital pulp therapy in permanent teeth[J]. Chin J Conserv Dent, 2017, 27(7): 410-417.
6 Chiang YC, Chang HH, Wong CC, et al. Nanocrystalline calcium sulfate/hydroxyapatite biphasic compound as a TGF-β1/VEGF reservoir for vital pulp therapy[J]. Dent Mater, 2016, 32(10): 1197-1208.
7 胡玉萍, 杨雪, 颜志豪, 等. PRF联合MTA用于兔牙直接盖髓术的影像学研究[J]. 口腔医学研究, 2021(7): 656-660.
Hu YP, Yang X, Yan ZH, et al. Radiographic study of PRF combined with MTA for direct pulp capping of rabbit teeth[J]. J Oral Sci Res, 2021(7): 656-660.
8 Tsigarida A, Chochlidakis K, Fraser D, et al. Peri-implant diseases and biologic complications at implant-supported fixed dental prostheses in partially edentulous patients[J]. J Prosthodont, 2020, 29(5): 429-435.
9 王瑾, 张月, 刘克达, 等. 应用浓缩生长因子促进干细胞增殖及成骨分化修复口腔颌面部缺损的研究进展[J]. 中国实用口腔科杂志, 2020(1): 47-50, 54.
Wang J, Zhang Y, Liu KD, et al. Research progress in the application of concentrated growth factor to promote stem cell proliferation and osteogenic differentiation in repairing oral and maxillofacial defects[J]. Chin J Pract Stomatol, 2020(1): 47-50, 54.
10 李佳, 何东宁. CGF在种植中促进成骨的研究探讨及应用进展[J]. 口腔颌面修复学杂志, 2021, 22(1): 76-80.
Li J, He DN. Research and application progress of CGF in promoting osteogenesis in implant[J]. Chin J Prosthodont, 2021, 22(1): 76-80.
11 Li Z, Liu L, Wang L, et al. The effects and potential applications of concentrated growth factor in dentin-pulp complex regeneration[J]. Stem Cell Res Ther, 2021, 12(1): 357.
12 Xu F, Qiao L, Zhao Y, et al. The potential application of concentrated growth factor in pulp regeneration: an in vitro and in vivo study[J]. Stem Cell Res Ther, 2019, 10(1): 134.
13 Assoian RK, Grotendorst GR, Miller DM, et al. Cellular transformation by coordinated action of three peptide growth factors from human platelets[J]. Nature, 1984, 309(5971): 804-806.
14 Wu PI, Diaz R, Borg-Stein J. Platelet-rich plasma[J]. Phys Med Rehabil Clin N Am, 2016, 27(4): 825-853.
15 Foster KN, Kim H, Potter K, et al. Acquired factor V deficiency associated with exposure to bovine thrombin in a burn patient[J]. J Burn Care Res, 2010, 31(2): 353-360.
16 Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part Ⅰ: technological concepts and evolution[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006, 101(3): e37-e44.
17 Kargarpour Z, Nasirzade J, Strauss FJ, et al. Platelet-rich fibrin suppresses in vitro osteoclastogenesis[J]. J Periodontol, 2020, 91(3): 413-421.
18 Agrawal AA. Evolution, current status and advances in application of platelet concentrate in periodontics and implantology[J]. World J Clin Cases, 2017, 5(5): 159-171
19 Rodella LF, Favero G, Boninsegna R, et al. Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction[J]. Microsc Res Tech, 2011, 74(8): 772-777.
20 Kawase T, Kamiya M, Kobayashi M, et al. The heat-compression technique for the conversion of platelet-rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation[J]. J Biomed Mater Res B Appl Biomater, 2015, 103(4): 825-831.
21 Sohn DS, Heo JU, Kwak DH, et al. Bone regeneration in the maxillary sinus using an autologous fib-rin-rich block with concentrated growth factors alone[J]. Implant Dent, 2011, 20(5): 389-395.
22 Honda H, Tamai N, Naka N, et al. Bone tissue engineering with bone marrow-derived stromal cells integrated with concentrated growth factor in Rattus norvegicus calvaria defect model[J]. J Artif Organs, 2013, 16(3): 305-315.
23 Wang F, Sun Y, He D, et al. Effect of concentrated growth factors on the repair of the goat temporomandibular joint[J]. J Oral Maxillofac Surg, 2017, 75(3): 498-507.
24 Jin R, Song G, Chai J, et al. Effects of concentrated growth factor on proliferation, migration, and differentiation of human dental pulp stem cells in vitro [J]. J Tissue Eng, 2018, 9: 2041731418817505.
25 Talaat WM, Ghoneim MM, Salah O, et al. Autologous bone marrow concentrates and concentrated growth factors accelerate bone regeneration after enucleation of mandibular pathologic lesions[J]. J Craniofac Surg, 2018, 29(4): 992-997.
26 Wang F, Li Q, Wang Z. A comparative study of the effect of Bio-Oss® in combination with concentrated growth factors or bone marrow-derived mesenchymal stem cells in canine sinus grafting[J]. J Oral Pathol Med, 2017, 46(7): 528-536.
27 Zhang M, Jiang F, Zhang X, et al. The effects of platelet-derived growth factor-BB on human dental pulp stem cells mediated dentin-pulp complex regeneration[J]. Stem Cells Transl Med, 2017, 6(12): 2126-2134.
28 Al-Hezaimi K, Naghshbandi J, Alhuzaimi R, et al. Regeneration of secondary dentin using recombinant human platelet-derived growth factor and MTA for pulp capping: a randomized controlled human clinical trial[J]. Int J Periodontics Restorative Dent, 2020, 40(4): 477-485.
29 Matsushita K, Motani R, Sakuta T, et al. The role of vascular endothelial growth factor in human dental pulp cells: induction of chemotaxis, proliferation, and differentiation and activation of the AP-1-dependent signaling pathway[J]. J Dent Res, 2000, 79(8): 1596-1603.
30 Borsani E, Bonazza V, Buffoli B, et al. Beneficial effects of concentrated growth factors and resveratrol on human osteoblasts in vitro treated with bisphosphonates[J]. Biomed Res Int, 2018, 2018: 4597321.
31 Kao CH. Use of concentrate growth factors gel or membrane in chronic wound healing: description of 18 cases[J]. Int Wound J, 2020, 17(1): 158-166.
32 Zhou R, Wang M, Zhang X, et al. Therapeutic effect of concentrated growth factor preparation on skin photoaging in a mouse model[J]. J Int Med Res, 2020, 48(10): 300060520962946.
33 Kamal A, Salman B, Abdul Razak NH, et al. The efficacy of concentrated growth factor in the healing of alveolar osteitis: a clinical study[J]. Int J Dent, 2020, 2020: 9038629.
34 Akcan SK, Ünsal B. Gingival recession treatment with concentrated growth factor membrane: a comparative clinical trial[J]. J Appl Oral Sci, 2020, 28: e20190236.
35 Pirpir C, Yilmaz O, Candirli C, et al. Evaluation of effectiveness of concentrated growth factor on osseointegration[J]. Int J Implant Dent, 2017, 3(1): 7.
36 Isler SC, Soysal F, Ceyhanlı T, et al. Regenerative surgical treatment of peri-implantitis using either a collagen membrane or concentrated growth factor: a 12-month randomized clinical trial[J]. Clin Implant Dent Relat Res, 2018, 20(5): 703-712.
37 Chen X, Wang J, Yu L, et al. Effect of concentrated growth factor (CGF) on the promotion of osteogenesis in bone marrow stromal cells (BMSC) in vivo [J]. Sci Rep, 2018, 8(1): 5876.
38 Yu M, Wang X, Liu Y, et al. Cytokine release kinetics of concentrated growth factors in different scaffolds[J]. Clin Oral Investig, 2019, 23(4): 1663-1671.
39 Bonazza V, Hajistilly C, Patel D, et al. Growth factors release from concentrated growth factors: effect of β-tricalcium phosphate addition[J]. J Craniofac Surg, 2018, 29(8): 2291-2295.
40 Dou L, Yan Q, Yang D. Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells[J]. Exp Ther Med, 2020, 19(3): 2377-2383.
41 Jun H, Lei D, Qifang Y, et al. Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study[J]. Braz Oral Res, 2018, 32: e48.
42 张诗韵, 赖光云, 汪俊. 浓缩生长因子与血凝块诱导根管内组织再生的对比研究[J]. 上海交通大学学报(医学版), 2020, 40(10): 1365-1370.
Zhang SY, Lai GY, Wang J. Comparative study on the regeneration capacity of tissue in root canals between concentrated growth factor and blood clot[J]. J Shanghai Jiaotong Univ (Med Sci), 2020, 40(10): 1365-1370.
43 Nivedhitha MS, Jacob B, Ranganath A. Concentrated growth factor: a novel platelet concentrate for revascularization of immature permanent teeth-a report of two cases[J]. Case Rep Dent, 2020, 2020: 1329145.
44 Tian S, Wang J, Dong F, et al. Concentrated growth factor promotes dental pulp cells proliferation and mineralization and facilitates recovery of dental pulp tissue[J]. Med Sci Monit, 2019, 25: 10016-10028.
[1] 郜慧慧,邓淑丽,何新敏,胡济安. 外伤年轻恒牙的牙髓状态判断及治疗选择[J]. 国际口腔医学杂志, 2021, 48(6): 675-682.
[2] 刘娟,陈斌,闫福华. 富血小板血浆和浓缩生长因子对人牙周膜细胞增殖和成骨分化影响的研究[J]. 国际口腔医学杂志, 2021, 48(5): 520-527.
[3] 魏中武,黄谢山,陈灼庚. 浓缩生长因子在口腔临床中的应用及研究进展[J]. 国际口腔医学杂志, 2020, 47(2): 235-243.
[4] 王瑜,王伟,顾新华. 浓缩生长因子在种植软硬组织增量方面的研究及应用[J]. 国际口腔医学杂志, 2019, 46(2): 218-222.
[5] 罗传霞1,2综述 林正梅1审校. 生物治疗在牙髓损伤修复中的应用研究[J]. 国际口腔医学杂志, 2012, 39(1): 48-51.
[6] 张莹. 牙髓组织损伤修复的机制[J]. 国际口腔医学杂志, 1999, 26(04): -.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 王昆润. 二甲亚砜和双氯芬酸并用治疗根尖周炎[J]. 国际口腔医学杂志, 1999, 26(06): .
[2] 汤庆奋,王学侠. 17β-雌二醇对人类阴道和口腔颊粘膜的渗透性[J]. 国际口腔医学杂志, 1999, 26(06): .
[3] 潘劲松. 颈总动脉指压和颈内动脉球囊阻断试验在大脑血液动力学中的不同影响[J]. 国际口腔医学杂志, 1999, 26(05): .
[4] 王昆润. 后牙冠根斜形牙折的治疗[J]. 国际口腔医学杂志, 1999, 26(05): .
[5] 杨锦波. 嵌合体防龋疫苗的研究进展[J]. 国际口腔医学杂志, 1999, 26(05): .
[6] 王昆润. 下颔骨成形术用网状钛板固定植骨块[J]. 国际口腔医学杂志, 1999, 26(04): .
[7] 汪月月,郭莉莉. 口腔机能与老化—痴呆危险因素流行病学研究[J]. 国际口腔医学杂志, 1999, 26(04): .
[8] 丁刚. 应用硬组织代用品种植体行丰颏术[J]. 国际口腔医学杂志, 1999, 26(04): .
[9] 田磊. 局部应用脂多糖后结合上皮反应性增生的变化[J]. 国际口腔医学杂志, 1999, 26(04): .
[10] 戴青. 口腔念珠菌病的新分类[J]. 国际口腔医学杂志, 1999, 26(04): .