国际口腔医学杂志

国际口腔医学杂志 ›› 2018, Vol. 45 ›› Issue (6): 734-738.doi: 10.7518/gjkq.2018.06.019

• 综述 • 上一篇    下一篇

石英晶体微天平在口腔医学研究中的应用

黄明娣,滕伟()   

  1. 中山大学光华口腔医学院·附属口腔医院口腔修复科·广东省口腔医学重点实验室 广州 510055
  • 收稿日期:2018-03-13 修回日期:2018-07-25 出版日期:2018-11-01 发布日期:2018-11-16
  • 通讯作者: 滕伟
  • 作者简介:黄明娣,医师,硕士,Email： 1476782968@qq.com
  • 基金资助:
    国家自然科学基金(81571015);国家自然科学基金(81371665);广东省自然科学基金(2015A030313087);广东省科技计划项目(2016A05050-2012);广州市科技计划(201707010011)

Application of quartz crystal microbalance in stomatological research

Mingdi Huang,Wei Teng()   

  1. Dept. of Prostho-dontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
  • Received:2018-03-13 Revised:2018-07-25 Online:2018-11-01 Published:2018-11-16
  • Contact: Wei Teng
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(81571015);This study was supported by the National Natural Science Foundation of China(81371665);Natural Science Foundation of Guangdong Province(2015A030313087);Guangdong Science and Technology Plan International Cooperation Project(2016A05050-2012);Guangzhou Science and Technology Program(201707010011)

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摘要：

石英晶体微天平（QCM）是一种高精度、实时测量的传感器,利用压电效应的原理检测痕量的变化。由于它能够检测到纳克级的质量变化,目前在分子生物学、疾病诊断和治疗、药物分析、有机化学、环境污染监测、食品卫生监督等领域得到了广泛的应用。本文主要阐述了QCM的工作原理以及其在口腔医学研究中的应用进展,包括QCM在聚电解质膜及涂层改性、蛋白吸附、种植体抗菌性、唾液、牙菌斑形成等方面的研究应用。

关键词: 石英晶体微天平, 界面, 质量

Abstract:

Quartz crystal microbalance (QCM) is a kind of sensor with high precision and real time measurement, and it detects the trace changes in quality using the principle of piezoelectric effect. It can perceive the quality change of the nanogram level, thus it’s widely used in molecular biology, diagnosis and treatment of disease, pharmaceutical analysis, organic chemistry, environmental monitoring, food hygiene supervision and other fields. This paper mainly introduces the detection principle of QCM and its applications in stomatological research, including polymer electrolyte membrane and coating modification, protein adsorption, implant antibacterial, saliva and dental plaque formation.

Key words: quartz crystal microbalance, interface, quality

中图分类号: 

  • R78
[1] Sauerbrey GZ . Use of quartz vibration for weighing thin films on a microbalance[J]. Z Phys, 1959,155:206-222.
doi: 10.1007/BF01337937
[2] 陈卓玥, 蔡玉龙, 张成 , 等. 石英晶体微天平应用于蛋白质吸附检测的研究[J]. 中国医疗器械信息, 2014,20(8):55-57, 62.
doi: 10.3969/j.issn.1006-6586.2014.08.013
Chen ZY, Cai YL, Zhang C , et al. Quantifying pro-tein adsorption on materials using QCM-D[J]. Chin Med Dev Inform, 2014,20(8):55-57, 62.
doi: 10.3969/j.issn.1006-6586.2014.08.013
[3] Kanazawa KK, Gordon II JG . The oscillation freque-ncy of a quartz resonator in contact with liquid[J]. Analyt Chim Acta, 1985,175(C):99-105.
doi: 10.1016/S0003-2670(00)82721-X
[4] Sommerfeld J, Richter J, Niepelt R , et al. Protein adsorption on nano-scaled, rippled TiO2 and Si sur-faces[J]. Biointerphases, 2012,7(1/2/3/4):55.
doi: 10.1007/s13758-012-0055-5
[5] Hamamoto R, Ito H, Hirohara M , et al. Interactions between protein molecules and the virus removal membrane surface: effects of immunoglobulin G adsorption and conformational changes on filter per-formance[J]. Biotechnol Prog, 2018,34(2):379-386.
doi: 10.1002/btpr.v34.2
[6] Wang D, Chen G, Wang H , et al. A reusable quartz crystal microbalance biosensor for highly specific detection of single-base DNA mutation[J]. Biosens Bioelectron, 2013,48:276-280.
doi: 10.1016/j.bios.2013.04.035 pmid: 23708749
[7] 滕伟, 黄洪章 . 聚电解质多层膜在钛表面改性中的应用[J]. 中华口腔医学杂志, 2013,48(10):636-639.
doi: 10.3760/cma.j.issn.1002-0098.2013.10.014
Teng W, Huang HZ . Polyelectrolyte multi-layer films for application in surface modification of titanium[J]. Chin J Stomatol, 2013,48(10):636-639.
doi: 10.3760/cma.j.issn.1002-0098.2013.10.014
[8] Teng W, Wang Q, Chen Y , et al. Controllably local gene delivery mediated by polyelectrolyte multilayer films assembled from gene-loaded nanopolymer-somes and hyaluronic acid[J]. Int J Nanomedicine, 2014,9:5013-5024.
doi: 10.2147/IJN.S70952 pmid: 4218923
[9] 滕伟, 王琴梅, 黄洪章 . 载基因纳米囊泡自组装多层膜组装行为研究[J/CD]. 中华口腔医学研究杂志(电子版), 2015,9(4):267-271.
doi: 10.3877/cma.j.issn.1674-1366.2015.04.002
Teng W, Wang QM, Huang HZ . Study on the asse-mbly behavior and mechanism of lipopolysacchar-ide-amine nanopolymersomes polyelectrolyte films [J/CD]. Chin J Stomatol Res (Electr Ed), 2015,9(4):267-271.
doi: 10.3877/cma.j.issn.1674-1366.2015.04.002
[10] Geißler S, Barrantes A, Tengvall P , et al. Deposition kinetics of bioinspired phenolic coatings on titanium surfaces[J]. Langmuir, 2016,32(32):8050-8060.
doi: 10.1021/acs.langmuir.6b01959 pmid: 5664158
[11] Zhou P, Deng Y, Lyu B , et al. Rapidly-deposited poly- dopamine coating via high temperature and vigorous stirring: formation, characterization and biofunctional evaluation[J]. PLoS One, 2014,9(11):e113087.
doi: 10.1371/journal.pone.0113087 pmid: 25415328
[12] Shons A, Dorman F, Najarian J . An immunospecific microbalance[J]. J Biomed Mater Res, 1972,6(6):565-570.
doi: 10.1002/(ISSN)1097-4636
[13] 秦泽曼, 滕伟 . 紫外线对钛表面蛋白吸附及竞争吸附的影响[J]. 中华口腔医学杂志, 2015,50(7):428-432.
doi: 10.3760/cma.j.issn.1002-0098.2015.07.010
Qin ZM, Teng W . Effect of ultraviolet-photofunc-tionalization of titanium on protein adsorption and competition[J]. Chin J Stomatol, 2015,50(7):428-432.
doi: 10.3760/cma.j.issn.1002-0098.2015.07.010
[14] Kokubu E, Yoshinari M, Matsuzaka K , et al. Beha-vior of rat periodontal ligament cells on fibroblast growth factor-2-immobilized titanium surfaces trea-ted by plasma modification[J]. J Biomed Mater Res A, 2009,91(1):69-75.
[15] Liu Z, Ma S, Duan S , et al. Modification of titanium substrates with chimeric peptides comprising anti-microbial and titanium-binding motifs connected by linkers to inhibit biofilm formation[J]. ACS Appl Mater Interfaces, 2016,8(8):5124-5136.
doi: 10.1021/acsami.5b11949 pmid: 26863404
[16] Yoshinari M, Kato T, Matsuzaka K , et al. Prevention of biofilm formation on titanium surfaces modified with conjugated molecules comprised of antimicro-bial and titanium-binding peptides[J]. Biofouling, 2010,26(1):103-110.
doi: 10.1080/08927010903216572 pmid: 20390560
[17] Ash A, Mulholland F, Burnett GR , et al. Structural and compositional changes in the salivary pellicle induced upon exposure to SDS and STP[J]. Biofouling, 2014,30(10):1183-1197.
doi: 10.1080/08927014.2014.977268 pmid: 25397690
[18] Ash A, Burnett GR, Parker R , et al. Structural charac- terisation of parotid and whole mouth salivary pellicles adsorbed onto DPI and QCMD hydroxyapatite sensors[J]. Colloids Surf B Biointerfaces, 2014,116:603-611.
doi: 10.1016/j.colsurfb.2013.10.024 pmid: 24269053
[19] Delvar A, Lindh L, Arnebrant T , et al. Interaction of polyelectrolytes with salivary pellicles on hydroxya-patite surfaces under erosive acidic conditions[J]. ACS Appl Mater Interfaces, 2015,7(38):21610-21618.
doi: 10.1021/acsami.5b07118 pmid: 26368580
[20] Veeregowda DH, van der Mei HC, Busscher HJ , et al. Influence of fluoride-detergent combinations on the visco-elasticity of adsorbed salivary protein films[J]. Eur J Oral Sci, 2011,119(1):21-26.
doi: 10.1111/j.1600-0722.2010.00798.x pmid: 21244507
[21] 姚江武, 张怡, 左起亮 . 3种巯基蛋白酶对去磷酸化牛乳β-酪蛋白和茶黄素复合膜的清除[J]. 口腔医学研究, 2014,30(1):9-12.
Yao JW, Zhang Y, Zuo QL . Effectiveness of three kinds of cysteine proteases on dephosphorylated bovine β-casein/theaflavin complex film removal[J]. J Oral Sci Res, 2014,30(1):9-12.
[22] 陈天来, 姚江武, 左起亮 . 不同条件下木瓜蛋白酶对唾液/色素复合膜的清除效果[J]. 口腔医学研究, 2014,30(1):27-29.
Chen TL, Yao JW, Zuo QL . Effectiveness of papain on saliva/pigment complex film removal under vari-ous conditions[J]. J Oral Sci Res, 2014,30(1):27-29.
[23] Yoshida E, Hayakawa T . Adsorption study of pellicle proteins to gold, silica and titanium by quartz crystal microbalance method[J]. Dent Mater J, 2013,32(6):883-887.
doi: 10.4012/dmj.2013-136 pmid: 24240906
[24] 陈国洋, 姚江武 . 绿茶多酚导致口腔收敛性感觉的吸附动力学研究[J]. 实用口腔医学杂志, 2011,27(1):21-25.
doi: 10.3969/j.issn.1001-3733.2011.01.04
Chen GY, Yao JW . Adsorption kinetic study of the oral astringency produced by polyphenols from green tea[J]. J Pract Stomatol, 2011,27(1):21-25.
doi: 10.3969/j.issn.1001-3733.2011.01.04
[25] Rupp F, Haupt M, Eichler M , et al. Formation and photocatalytic decomposition of a pellicle on anatase surfaces[J]. J Dent Res, 2012,91(1):104-109.
doi: 10.1177/0022034511424901 pmid: 21979134
[26] Bai L, Takagi S, Guo Y , et al. Inhibition of Streptoco-ccus mutans biofilm by LL-37[J]. Int J Med Sci Bio-technol, 2013,1(1):56-64.
[27] Yoshida E, Hayakawa T . Adsorption analysis of lac-toferrin to titanium, stainless steel, zirconia, and polymethyl methacrylate using the quartz crystal microbalance method[J]. Biomed Res Int, 2016,2016:3961286.
[28] Westas E, Svanborg LM, Wallin P , et al. Using QCMD to study the adhesion of human gingival fibroblasts on implant surfaces[J]. J Biomed Mater Res A, 2015,103(10):3139-3147.
doi: 10.1002/jbm.a.35458 pmid: 25779215
[29] Connelly C, Cicuto T, Leavitt J , et al. Dynamic inter-actions of amelogenin with hydroxyapatite surfaces are dependent on protein phosphorylation and solution pH[J]. Colloids Surf B Biointerfaces, 2016,148:377-384.
doi: 10.1016/j.colsurfb.2016.09.010 pmid: 27632699
[30] Cecchinato F, Atefyekta S, Wennerberg A , et al. Modu- lation of the nanometer pore size improves magne-sium adsorption into mesoporous titania coatings and promotes bone morphogenic protein 4 expression in adhering osteoblasts[J]. Dent Mater, 2016,32(7):e148-e158.
doi: 10.1016/j.dental.2016.04.002
[31] Lima MA, Bastos IN, Cella N . Quartz crystal micro-balance and photoacoustic measurements in dental photocuring[J]. Rev Sci Instrum, 2016,87(9):093903.
doi: 10.1063/1.4963309 pmid: 27782581
[32] Zhou Y, Zhou P, Xin Y , et al. Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microba-lance based assay[J]. Sci Rep, 2014,4:6978.
doi: 10.1038/srep06978 pmid: 25381797
[33] Elsom J, Lethem MI, Rees GD , et al. Novel quartz crystal microbalance based biosensor for detection of oral epithelial cell-microparticle interaction in real- time[J]. Biosens Bioelectron, 2008,23(8):1259-1265.
doi: 10.1016/j.bios.2007.11.020 pmid: 18207383
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[4] 王昆润. 重型颌面部炎症死亡和康复病例的实验室检查指标比较[J]. 国际口腔医学杂志, 1999, 26(06): .
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[6] 温秀杰. 氟化物对牙本质脱矿抑制作用的体外实验研究[J]. 国际口腔医学杂志, 1999, 26(05): .
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